Development and validation of a RP-HPLC method for determination of atorvastatin calcium and aspirin in a capsule dosage form

Abstract

A simple, specifi c and accurate reverse phase high performance liquid chromatographic method was developed for the simultaneous determination of atorvastatin calcium and aspirin in capsule dosage forms. A phenomenex Gemini C-18, 5 µm column having 250 x 4.6 mm i.d. in isocratic mode, with mobile phase containing 0.02 M potassiumdihydrogen phosphate: methanol (20:80) adjusted to pH 4 using ortho phosphoric acid was used. The fl ow rate was 1.0 ml/ min and effl uents were monitored at 240 nm. The retention times of atorvastatin calcium and aspirin were 5.4 min and 3.4 min, respectively. The linearity for atorvastatin calcium and aspirin were in the range of 0.5-4 µg/ml and 5-25 µg/ml, respectively. The recoveries of atorvastatin calcium and aspirin were found to be in the range of 98.02-100.68% and 98.38-101.42%, respectively. The proposed method was validated and successfully applied to the estimation of atorvastatin calcium and aspirin in combined capsule dosage forms.

Quantitative Simultaneous Determination of Fenofibrate, Atorvastatin, and Ezetimibe in Tablets Using Gradient High-Performance Column Liquid Chromatography and High-Performance Thin-Layer Chromatography

“…Different analytical methods for determination of ATO in biological fluids have been reported and include HPLC-UV and HPTLC. [4][5][6][7] ATO has been assayed in pharmaceutical formulations by HPLC, [8][9][10][11][12][13][14][15][16][17][18][19][20] HPTLC, [21][22][23] spectrometry, [24] and capillary zone electrophoresis methods. [25] Analytical methods that have been reported for the determination of FEN in formulations [26][27][28][29][30][31] and biological fluids [32,33] include HPLC, HPLC=MS, HPTLC, and UV spectrometry.…”

Quantification of atorvastatin calcium in tablets by FT-Raman spectroscopy

“…Chromatographic techniques such as HPLC [2][3][4][5][6] and LC-MS [7,8] are the most popular analytical methods for this active pharmaceutical ingredient (API) quantification. Other analytical techniques used for ATC quantification include electrophoresis [9] and UV-vis spectrometry [10][11][12].…”

New Kinetic Spectrophotometric Method for Determination of Atorvastatin in Pure and Pharmaceutical Dosage Forms

“…Atorvastatin calcium {[R-(R, R*)]-2-(4-flurophenyl)-β,δ-dihydroxy-5(1-methylethyl)-3-phenyl-4-[phenylamino)carbonyl]-1H-pyrrole-1-heptanoic acid, calcium salt (2:1) trihydrate} is the most commonly occurring drug in commercially available pharmaceutical formulations used for the clinical treatment of hypercholesterolemia [1]. Several methods have been described for the quantitative determination of atorvastatin by high-performance liquid chromatography (HPLC) in different pharmaceutical preparations, either alone [2][3][4][5][6][7] or with other active ingredients [8][9][10][11][12][13][14][15][16]. HPTLC [17], electrochemical [18,19], capillary electrophoresis [20] and spectrofluorimetric [21] methods have been developed for the analysis of atorvastatin from its individual and combined formulations with other active ingredients in pharmaceutical preparations and plasma.…”