Development and Validation of a TLC-Densitometry Method for Histamine Monitoring in Fish and Fishery Products

Abstract

Histamine poisoning is a significant public health problem. Therefore, the monitoring of histamine content in fish and fishery products is considered to be a crucial measure in the seafood industry. In the present study, a simple and rapid densitometric thin-layer chromatographic (TLC) method for histamine determination in fish samples was developed and validated. The samples were homogenized with 10% trichloroacetic acid and histamine was efficiently extracted. Then, an appropriate derivatization procedure was adopted with dansyl chloride. Once the derivatization was carried out, the samples were applied to silica gel TLC plates and developed by ascending chromatography with chloroform-triethylamine (6:4, v/v) as the mobile phase. The intensity of the histamine-dansyl derivative spots was measured by densitometry at 365 nm, and the quantitation was performed by BIO-1D image processing software. The validation of this method revealed good linearity and specificity over a concentration range from 6.25 to 100 mg/kg. Adequate precision was shown by relative standard deviations (RSD) smaller than 4.82%, accuracy ranged from −6.88% to 5.28%, and satisfactory recoveries ranging from 93% to 105% were obtained. The Limit of Detection and the Limit of Quantification were calculated at 4.4 mg/kg and 10.5 mg/kg, respectively. In addition, the effectiveness of the proposed method was assessed by the analysis of various samples, and the obtained results were confirmed with those achieved by the HPLC-UV method. Moreover, the developed method was found to be simple, cheap, and suitable for application to analyze several samples simultaneously.

Dual-Mode Separation and SERS Detection of Carbaryl with PA-6/AuNRs@ZIF-8 Films

“…Metal–organic frameworks (MOFs) consist of metal ions and organic ligands bonded by coordination and have a large internal specific surface area and the ability of molecule separation. − Encapsulating metal nanoparticles into MOFs as a core–shell structure can be conducive to enhanced selectivity and stability in applications such as SERS sensing. Zeolitic imidazolate-based MOFs (ZIF-8), which have the smallest aperture size, encapsulated on single golden nanorods (AuNRs) can be a promising SERS substrate to capture the carbaryl in intricate surroundings. , Thin layer chromatography (TLC) is a simple separation tool with the advantages of portability, ease of operation, and low economic cost. , The combination of thin-layer chromatography and ZIF-8 can fulfill the dual-mode separation and rapid on-site detection of toxicants in a short time …”

“…20,21 Thin layer chromatography (TLC) is a simple separation tool with the advantages of portability, ease of operation, and low economic cost. 22,23 The combination of thin-layer chromatography and ZIF-8 can fulfill the dual-mode separation and rapid on-site detection of toxicants in a short time. 24 In this case, polyamide 6 (PA-6) was used as a TLC substrate.…”

Phase transferred and non-coated, water soluble perovskite quantum dots for biocompatibility and sensing

“…24 Normal levels of HA in human blood range from 300 to 900 nM, while it is 350 to 500 nM in urine. The presence of HA cannot be recognised easily, and at present, methods like electrochemical sensing, high-performance liquid chromatography (HPLC), 25 thin-layer chromatography (TLC), 26 enzyme linked immunosorbent assay (ELISA), 27 gas chromatography (GC) 28 and capillary zone electrophoresis, 29 which involve high cost, experienced handling, more time and large laboratory requirements, 30 are used. Herein fluorescent based optical sensors using QDs could detect very low levels of HA with additional advantages like high efficiency, low cost, high sensitivity and rapid detection.…”

Histamine Control in Raw and Processed Tuna: A Rapid Tool Based on NIR Spectroscopy

“…Historically, several analytical methods have been developed for monitoring histamine concentrations in food items, which are mainly based on electrophoretic [ 12 ], fluorometric [ 13 ], enzymatic or immunoassay detection [ 14 ] and chromatographic separation by thin-layer chromatography [ 15 ], high performance liquid chromatography (HPLC) [ 16 ] or gas chromatography [ 17 ], and, recently, also biosensors [ 18 ].…”