Development and validation of a hydrophilic interaction liquid chromatography–tandem mass spectrometry method for determination of isoniazid in human plasma

Huang, Liusheng; Marzan, Florence; Jayewardene, Anura L.; Lizak, Patricia; Li, Xiaohua; Aweeka, Francesca

doi:10.1016/j.jchromb.2008.12.024

Cited by 37 publications

(30 citation statements)

References 9 publications

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“…Bench-top stability testing and the more extensive stability assessment showed that isoniazid is unstable in human serum unless it is freeze-dried or stored at -80°C. Instability of isoniazid in plasma at ambient temperature and -20ºC has been reported earlier [28,29]. This instability calls for adequate standard operating procedures and rapid handling of samples.…”

Section: Discussionmentioning

confidence: 99%

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Quantification of isoniazid, pyrazinamide and ethambutol in serum using liquid chromatography-tandem mass spectrometry

Sturkenboom¹,

Lijke²,

Jongedijk³

et al. 2015

J Appl Bioanal

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Clinical studies on tuberculosis have shown a correlation between low drug exposure and treatment failure and acquired drug resistance. Objective was to develop a LC-MS/MS method for the quantification of isoniazid, pyrazinamide and ethambutol. Stable isotope-labelled isoniazid-D4 and ethambutol-D4 were used as internal standards. Protein binding of isoniazid, pyrazinamide and ethambutol was investigated and proved low. Therefore, sample preparation using ultrafiltration could be applied, resulting in linear calibration curves in the range of 0.2-8 mg/L for isoniazid and ethambutol and 2-80 mg/L for pyrazinamide. The method was validated according to the guidelines of the FDA. A fast, simple and reliable LC-MS/MS method has been developed for the simultaneous determination of isoniazid, pyrazinamide and ethambutol in human serum for therapeutic drug monitoring and pharmacokinetic studies.

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Section: Discussionmentioning

confidence: 99%

“…Therefore, an experiment in fivefold was performed to evaluate the magnitude of the carry-over by injecting five LLOQ (Lower Limit of Quantification) samples after a HLQ [28,29]. The total amount of stock solutions added to the serum was less than 5% of the final volume.…”

Section: Carry-overmentioning

confidence: 99%

Quantification of isoniazid, pyrazinamide and ethambutol in serum using liquid chromatography-tandem mass spectrometry

Sturkenboom¹,

Lijke²,

Jongedijk³

et al. 2015

J Appl Bioanal

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“…HPLC-UV [7][8][9][10][11] and HPLC-MS [12,13] methods using RP sorbents dominate the simultaneous analysis of INH and AcINH. Hydrophilic interaction liquid chromatography (HILIC) on Hypersil silica with MS detection has been also developed for determination of INH in plasma [14]. Vitamin B6 is typically analyzed in RP settings after pre or post column derivatization using fluorescent [15][16][17][18][19] or electrochemical detections [20].…”

Section: Introductionmentioning

confidence: 99%

Use of different stationary phases for separation of isoniazid, its metabolites and vitamin B6 forms

Pasáková

Gladziszová

Charvátová

et al. 2011

J of Separation Science

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The ability of different stationary phases developed for the analysis of polar compounds (ZIC-HILIC, ZIC-pHILIC and Zorbax SB-Aq) to separate isoniazid, its metabolites (acetylisonazid, pyridoxal isonicotinoyl hydrazone, pyridoxal isonicotinoyl hydrazone 5-phosphate), pyridoxine, pyridoxal and pyridoxal 5-phosphate under MS compatible conditions was systematically investigated using HPLC-UV. The mobile phase strength, pH and buffer concentration were modified to assess their impact on the retention of these compounds. The best available separation of the compounds was achieved using 1 mM ammonium formate (pH≈6) and ACN (20:80, v/v) on ZIC-HILIC and employing 5 mM ammonium formate (pH 3.0) and ACN (40:60, v/v) on ZIC-pHILIC. A gradient profile using 0.5 mM ammonium formate (pH≈6) and MeOH (0-12 min: 10% MeOH, 12-15 min: 10-50% MeOH, 15-35 min: 50% MeOH, 35.0-35.2 min: 50-10% MeOH, 35.2-45.0 min: 10% MeOH) provided the best separation of the compounds on Zorbax SB-Aq. Subsequent LC-MS analysis demonstrated that ZIC-HILIC is useful for the analysis of pyridoxine, pyridoxal and pyridoxal isonicotinoyl hydrazone. However, the chromatographic conditions developed for the analysis of the compounds on Zorbax SB-Aq are capable of achieving the best separation of all compounds in this study with the higher sensitivity for most of the analytes.

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“…It then had to be injected two times for each sample to analyze PZA, INH and RFP. LC-MS is selective and sensitive, and several HPLC-MS methods 17,[19][20][21][22][23] have been reported. However, it is very expensive and not always available, particularly in remote areas of developing countries.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Isoniazid, Pyrazinamide, Rifampicin and Acetylisoniazid in Human Plasma by High-Performance Liquid Chromatography

et al. 2010

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A sensitive and accurate high-performance liquid-chromatography method was developed and validated for the simultaneous determination of pyrazinamide (PZA), isoniazid (INH), rifampicin (RFP) and acetylisoniazid (AcINH) in human plasma. Separation was performed on a Max-RP C12 column using gradient elution and a flow-rate program. The mobile phase was methanol-acetonitrile-buffer (20 mM of heptanesulfonic acid sodium, pH 2.5) with a ratio of 10:8:82 (v/v/v) at the initial phase. All calibration curves had good linearity (r 2 > 0.99) between the test ranges. The intra-and inter-day precision was less than 8.8% in good accuracy (<15%). The limit of detection with a signal-to-noise (S/N) of 3 was 0.014, 0.009, 0.023 and 0.054 µg mL -1 for PZA, AcINH, INH and RFP, respectively. The method was selective, sensitive and reliable, and is a good alternative for routine therapeutic drug monitoring of the four compounds during the treatment of tuberculosis patients.

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Development and validation of a hydrophilic interaction liquid chromatography–tandem mass spectrometry method for determination of isoniazid in human plasma

Cited by 37 publications

References 9 publications

Quantification of isoniazid, pyrazinamide and ethambutol in serum using liquid chromatography-tandem mass spectrometry

Quantification of isoniazid, pyrazinamide and ethambutol in serum using liquid chromatography-tandem mass spectrometry

Use of different stationary phases for separation of isoniazid, its metabolites and vitamin B6 forms

Simultaneous Determination of Isoniazid, Pyrazinamide, Rifampicin and Acetylisoniazid in Human Plasma by High-Performance Liquid Chromatography

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