Development and validation of a high‐performance liquid chromatography–tandem mass spectrometric method for simultaneous determination of bupropion, quetiapine and escitalopram in human plasma

Park, Semin; Park, Chul‐Soo; Lee, Sung Joong; Cha, Boseok; Cho, Young Ah; Song, Yi; Yu, Eun Ae; Kim, Gon‐Sup; Jin, Jong Sung; El‐Aty, A. M. Abd; El-Banna, H. A.; Hacımüftüoğlu, Ahmet; Shin, Sung Chul

doi:10.1002/bmc.3495

Cited by 4 publications

(6 citation statements)

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“…An optimized chromatography separation and mass spectrometry detection could facilitate the drug quantification and analysis process. The first way to achieve the goal is to find a better mobile phase and the buffer substance, and the buffer substances could adjust the mobile phases pH and enhance the ionization efficiency to obtain a better peak symmetry and higher responses [31]. For the condition optimization, different buffer substances, for example, formic acid (0.05%, 0.1%, 0.2%, and 0.5%; V/V), acetic acid (0.1%; V/V), ammonium acetate (5 mmol/L, 10 mmol/L), were added for the tailing suppression and response enhancement.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous Quantification of Methotrexate and Its Metabolite 7-Hydroxy-Methotrexate in Human Plasma for Therapeutic Drug Monitoring

Ren

Wang

Yun

et al. 2019

International Journal of Analytical Chemistry

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Objective. To establish and validate a simple, sensitive, and rapid liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of methotrexate (MTX) and its major metabolite 7-hydroxy-methotrexate (7-OH-MTX) in human plasma. Method. The chromatographic separation was achieved on a Zorbax C18 column (3.5 μm, 2.1 × 100 mm) using a gradient elution with methanol (phase B) and 0.2% formic acid aqueous solution (phase A). The flow rate was 0.3 mL/min with analytical time of 3.5 min. Mass spectrometry detection was performed in a triple-quadruple tandem mass spectrometer under positive ion mode with the following mass transitions: m/z 455.1/308.1 for MTX, 471.0/324.1 for 7-OH-MTX, and 458.2/311.1 for internal standard. The pretreatment procedure was optimized with dilution after one-step protein precipitation. Results. The calibration range of methotrexate and 7-OH-MTX was 5.0-10000.0 ng/mL. The intraday and interday precision and accuracy were less than 15% and within ±15% for both analytes. The recovery for MTX and 7-OH-MTX was more than 90% and the matrix effect ranged from 97.90% to 117.60%. Conclusion. The method was successfully developed and applied to the routine therapeutic drug monitoring of MTX and 7-OH-MTX in human plasma.

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Section: Resultsmentioning

confidence: 99%

Simultaneous Quantification of Methotrexate and Its Metabolite 7-Hydroxy-Methotrexate in Human Plasma for Therapeutic Drug Monitoring

Ren

Wang

Yun

et al. 2019

International Journal of Analytical Chemistry

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“…The method was validated, and the m/z was 166, 184 and 240 for the analyte and 185 and 349.2 for the IS, respectively [48]. Other LC-MS/MS methods for the quantification of BUP in human plasma [150][151][152][153][154][155][156][157] and serum samples [158] are reported in Table 2.…”

Section: Liquid Chromatography (Lc)mentioning

confidence: 99%

Review on analytical methods for quantification of ADHD drugs in human biological samples

Sundari¹,

Amuthalakshmi²,

Nalini³

2020

Reviews in Analytical Chemistry

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Attention deficit hyperactivity disorder (ADHD) is a common neuro-developmental disorder. The symptoms of ADHD include difficulty in attention, memory and impulse control. Many pharmaceutical formulations (stimulants and non-stimulants) are available on the market to treat ADHD symptoms. The most commonly used drugs for treatment are amphetamine, methylphenidate, atomoxetine, bupropion, guanfacine and clonidine. In the field of pharmaceuticals, bioanalysis is an important tool used for the quantification of drugs and their metabolites present in biological samples using various analytical methods. Although a number of analytical methods were reported for the quantification of these drugs in biological samples of experimental animals, due to species differences, it is important to develop analytical methods to quantify these drugs in human biological samples to aid forensic and pharmacokinetic studies. In this review, we compile the bio-analytical methods such as spectrophotometry, spectrofluorimetry, mass spectrometry, electrophoresis, liquid chromatography and gas chromatography used for the quantification of ADHD drugs in human biological samples such as blood, plasma, serum, oral fluids, sweat, hair and urine based on earlier published articles from various journals.

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“…Several mass spectrometry-or high performance liquid chromatography (HPLC)-based methods have been published for atomoxetine or escitalopram, neither of those has been specified as suitable for both drugs. Though, escitalopram in plasma has been determined by several LC-MS methods, such as LC-ESI-MS with LLOQ 1 ng/ml following L/L extraction or directly electro-sprayed into a (time of flight) TOF-MS [10,11] or with LLOQ of 0.2 ng/ml by LC-Tandem MS following liquid extraction and evaporation [12,13], or liquid extraction plus SPE clean up and gradient flow conditions [14] as well as direct injection following protein denaturation and evaporation [15] , both without data on method evaluation. More toxicological and forensic directed LC-MS methods for determination of escitalopram in neonatal hair (LOQ 25 pg/ml) [16] or in postmortem human samples [17] have also been reported.…”

Section: Introductionmentioning

confidence: 99%

Determination of atomoxetine or escitalopram in human plasma by HPLC: Applications in neuroscience research studies

Teichert

Rowe

Ersche

et al. 2020

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Background: Atomoxetine and escitalopram are potent and selective drugs approved for noradrenergic or serotonergic modulation of neuronal networks in attention-deficit hyperactivity disorder (ADHD) or depression, respectively. High-performance liquid chromatography (HPLC) methods still play an important role in the Therapeutic Drug Monitoring (TDM) of psychopharmacological drugs, and coupled with tandem mass spectrometry are the gold standard for the quantification of drugs in biological matrices, but not available everywhere. The aim of this work was to develop and validate a HPLC method for neuroscientific studies using atomoxetine or escitalopram as a test drug.Methods: A HPLC method from routine TDM determination of atomoxetine or citalopram in plasma was adapted and validated for use in neuroscientific research. Using photo diode array detection with UV absorption at 205 nm, the variation of internal standard within one chromatographic method enables separate drug monitoring for concentration-controlled explorative studies in healthy humans and patients with Parkinson's disease. Results:The method described here was found to be linear in the range of 0.002 -1.4 mg/L for atomoxetine and 0.0012 -0.197 mg/L for escitalopram, with overall mean intra-day and inter-day imprecision and accuracy bias < 10% for both drugs. The method was successfully applied in concentration-controlled neuroimaging studies in populations of healthy humans and patients with Parkinson's disease. Conclusion:A simple, sensitive, robust HPLC method capable of monitoring escitalopram and atomoxetine is presented and validated, as a useful tool for drug monitoring and the study of pharmacokinetics in neuroscientific study applications. Statement on Effects of EthnicityThis analytical in vitro method described here in its validation performance is not affected by ethnicity. Quite in contrast the method is suitable to map the different pharmacokinetics in different ethnicities based on variations in genotype of metabolizing cytochromes CYP2D6 which or CYP2C19 relevant for atomoxetine and escitalopram. In this study, plasma concentrations from healthy volunteers, cocaine-dependent individuals and patients with Parkinson's disease were predominantly obtained from a Caucasian population.

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Development and validation of a high‐performance liquid chromatography–tandem mass spectrometric method for simultaneous determination of bupropion, quetiapine and escitalopram in human plasma

Abstract: Shin SC. Development and validation of a high-performance liquid chromatography-tandem mass spectrometric method for simultaneous determination of bupropion, quetiapine and escitalopram in human plasma. Biomedical Chromatography 2015; 29: 612-618.

Cited by 4 publications

References 1 publication

Simultaneous Quantification of Methotrexate and Its Metabolite 7-Hydroxy-Methotrexate in Human Plasma for Therapeutic Drug Monitoring

Simultaneous Quantification of Methotrexate and Its Metabolite 7-Hydroxy-Methotrexate in Human Plasma for Therapeutic Drug Monitoring

Review on analytical methods for quantification of ADHD drugs in human biological samples

Determination of atomoxetine or escitalopram in human plasma by HPLC: Applications in neuroscience research studies

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