Development and validation of a bioanalytical assay for the measurement of total and unbound teicoplanin in human serum

Abstract

Background The glycopeptide teicoplanin is considered first-line treatment for severe infections caused by Gram-positive bacteria. Individualized treatment of teicoplanin is gaining interest. As only protein-unbound drug is pharmacologically active, a sensitive assay measuring unbound and total teicoplanin is indispensable for pharmacological research and dose optimization. Objectives To develop and validate a UPLC-MS/MS method to quantify unbound and total teicoplanin in human serum. Methods The developed assay was validated according to the ICH guideline M10 on Bioanalytical Method Validation and study sample analysis. Unbound teicoplanin was obtained by ultrafiltration. The assay was cross-validated with a quantitative microsphere (QMS) immunoassay in a side-by-side comparison using 40 patient samples. Results With the developed and validated method, all main teicoplanin components (A2-1, A2-2/A2-3, A2-4/A2-5 and A3-1) can be quantified. Total run time was 5.5 min. Concentration range was 2.5–150 mg/L for total and 0.1–25 mg/L for unbound teicoplanin. Precision (coefficient of variation) and accuracy (bias) of total teicoplanin were 5.97% and 107%, respectively, and 7.17% and 108%, respectively, for unbound teicoplanin. Bland–Altman analysis showed total concentrations measured with the UPLC-MS/MS method were equivalent to the results of the QMS immunoassay. A total of 188 samples from 30 patients admitted to the ICU and haematology department were measured; total concentrations ranged between 2.92 and 98.5 mg/L, and unbound concentrations ranged between 0.37 and 30.7 mg/L. Conclusions The developed method provided rapid, precise and accurate measurement of unbound and total teicoplanin. The developed method is now routinely applied in pharmacological research and clinical practice.

Necessity for higher teicoplanin doses in older adults: a multicenter prospective observational study in China

“…Samples were promptly refrigerated and centrifuged at 2500 × g for 10 min before 2 mL of supernatant was preserved at -20 °C for subsequent analysis. Teicoplanin concentrations were determined using liquid chromatography-tandem mass spectrometry [20][21][22]. The linear range of the method was 1.0-100.0 mg/L, and the lower limit of quantification was 1.0 mg/L.…”