Development and validation of a liquid chromatographic method for the simultaneous determination of four anthracyclines and their respective 13-S-dihydro metabolites in plasma and saliva

Maudens, Kristof E.; Stove, Christophe; Cocquyt, Véronique; Denys, Hannelore; Lambert, Willy E.

doi:10.1016/j.jchromb.2009.09.044

Cited by 37 publications

(34 citation statements)

References 34 publications

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“…However, we found the pharmacokinetic profiles from epirubicin-treated patients to contain a slight increase 4h post-I.V. infusion, which may be indicative that enterohepatic recycling may exist [13].…”

Section: Introductionmentioning

confidence: 67%

“…One method could be applied to each of the four pairs (main compound and respective reduced metabolite) individually [68]. Four methods were developed that could determine simultaneously two or more main compounds alone [69] or together with their reduced metabolites [13,[70][71][72].…”

Section: Analytes and Concentrations Of Interestmentioning

confidence: 99%

“…As organic solvent, acetonitrile (pure or in combination with an acidic buffer or acid) [53,54,58,64], methanol [59,62] and ethanol [13] have been used.…”

Section: Deproteinizationmentioning

confidence: 99%

“…Two major strategies have been followed: (1) an extraction followed by an evaporation step [13,42,61,63] or (2) an extraction followed by a back-extraction [27,53,68]. Since the first step is in both cases similar, this will be discussed together.…”

Section: Liquid-liquid Extractionmentioning

confidence: 99%

“…Ethylacetate [53], dichloromethane [13], and mixtures of chloroform with n-propanol [61,63] isopropanol [27,42] or 1-heptanol [68] have been used as extractants, mostly after addition of a mild alkaline buffer (buffer pH-range 8.5 to 9.5) to obtain high recoveries.…”

Section: Extraction Into An Organic Solvent (Mixture)mentioning

confidence: 99%

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Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Maudens

Stove

Lambert

2011

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 67%

Section: Analytes and Concentrations Of Interestmentioning

confidence: 99%

“…As organic solvent, acetonitrile (pure or in combination with an acidic buffer or acid) [53,54,58,64], methanol [59,62] and ethanol [13] have been used.…”

Section: Deproteinizationmentioning

confidence: 99%

Section: Liquid-liquid Extractionmentioning

confidence: 99%

Section: Extraction Into An Organic Solvent (Mixture)mentioning

confidence: 99%

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Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Maudens

Stove

Lambert

2011

Journal of Chromatography B

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Enhancing Doxorubicin Detection: Multiphase Electroextraction for Efficient and Affordable UHPLC–DAD Analysis in Saliva

Sousa,

Pereira,

Orlando

2024

Electrophoresis

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Attesting optimal drug concentrations in biological fluids is crucial to ensure precise dosage adjustment, to guarantee therapy adherence, and to manage side effects in chemotherapy. Accurate drug determination relies on liquid chromatography and advanced detectors, with sample preparation playing a pivotal role, especially in complex matrices such as biological fluids. This study introduces a multiphase electroextraction (MPEE) of doxorubicin (DOX) in saliva by utilizing a paper point, followed by ultra‐high‐performance liquid chromatography coupled to diode array detection. The extraction time and electric potential were carried out by using the Doehlert optimization approach, whereas the desorption solvent was fine‐tuned through the centroid–simplex experimental design. After optimization, DOX and the internal standard were extracted in 35 min, utilizing an applied voltage of 300 V and a multiwell plate capable of simultaneous extraction of 66 samples. The recovery was 87%–101%, with a linear range between 50 and 500 µg L−1 (R2 > 0.999). The intra‐ and inter‐assay coefficients of variation for precision were <10%, and the limit of detection and limit of quantification were 25 and 50 µg L−¹, respectively. When applied to five different fortified saliva samples, there were no statistically significant differences in the detected concentrations. Although the enrichment factor (0.6) was not as high as expected, the other results confirm that the method obtained is suitable for monitoring DOX in this complex matrix and can contribute to further developments in sample preparation using MPEE approaches.

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Quantitative determination of Bisphenol A from human saliva using bulk derivatization and trap‐and‐elute liquid chromatography coupled to electrospray ionization mass spectrometry

Yang

Morgan

Nguyen

et al. 2011

Enviro Toxic and Chemistry

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Endocrine disruptors cause adverse health effects as a result of their ability to shift the hormonal balance that is essential to the body. Bisphenol A (BPA) is an endocrine disruptor that has garnered much attention because of its presence in many consumer materials, which generates a significant risk for exposure. A method is presented for rapid detection of oral exposure to BPA directly from human saliva. Saliva was chosen because it serves as a noninvasive sampling route to detect BPA exposure; however, it is one of many complex biological matrices that have traditionally posed problems in quantitative analysis. Such analyses usually require extensive sample preparation to reduce interferences contributed by the sample matrix. Three validated methods are presented here that feature a streamlined sample-preparation strategy (bulk derivatization) prior to accurate and sensitive analysis by trap-and-elute liquid chromatography coupled to electrospray ionization mass spectrometry. Validated methods include standard addition calibration with variable injection volumes and multiple injection loading, as well as with incorporation of an internal standard. Reported limits of detection reached as low as 49.0 pg/ml (2.9 pg loaded on-column; equivalent to parts per trillion in saliva) among the presented methods with good accuracy and precision throughout. A proof-of-concept study is demonstrated to show that the final validated method has potential application to specific studies for trace-level BPA detection from real samples.

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Development and validation of a liquid chromatographic method for the simultaneous determination of four anthracyclines and their respective 13-S-dihydro metabolites in plasma and saliva

Cited by 37 publications

References 34 publications

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Enhancing Doxorubicin Detection: Multiphase Electroextraction for Efficient and Affordable UHPLC–DAD Analysis in Saliva

Quantitative determination of Bisphenol A from human saliva using bulk derivatization and trap‐and‐elute liquid chromatography coupled to electrospray ionization mass spectrometry

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