2020
DOI: 10.1016/j.biochi.2020.03.010
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Development and validation of a rapid, specific and sensitive LC-MS/MS bioanalytical method for eicosanoid quantification - assessment of arachidonic acid metabolic pathway activity in hypertensive rats

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Cited by 15 publications
(20 citation statements)
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“…Analytes were separated under the gradient elution mode. The MS detection of studied eicosanoid and their deuterated internal standards was carried out in negative ion electrospray ionisation by applying the multiple reaction monitoring mode (MRM); this method and the instrument operation parameters were described previously [ 20 ].…”
Section: Methodsmentioning
confidence: 99%
“…Analytes were separated under the gradient elution mode. The MS detection of studied eicosanoid and their deuterated internal standards was carried out in negative ion electrospray ionisation by applying the multiple reaction monitoring mode (MRM); this method and the instrument operation parameters were described previously [ 20 ].…”
Section: Methodsmentioning
confidence: 99%
“…Selected eicosanoids (5-, 12-, 15-, and 20-HETE, 8,9-, 11,12-, and 14,15-EET, 8,9-, 11,12-, and 14,15-DHET) were quantified in plasma, Xyzyk-derived plasma, and Krebs-Hepes buffer collected after aorta incubation using a UPLC-MS/MS technique with the application of an already published methodology [53]. In short, each sample was spiked with a mixture of internal standards and gently mixed.…”
Section: Uplc-ms/ms Eicosanoid Analysismentioning
confidence: 99%
“…Plasma and urine samples were prepared for the LC-MS/MS assay according to the protocol slightly changed from that previously described in [22,23]. Briefly, after the addition of internal standard mixture, all samples were purified applying liquid-liquid extraction using acidified ethyl acetate.…”
Section: Measurement Of Eicosanoid Production By Lc-ms/msmentioning
confidence: 99%
“…Calibration and quality control samples were prepared using artificial plasma or urine [22,23] spiked with known concentration of eicosanoid working standard solutions and extracted according to the same procedure applied for the biological samples.…”
Section: Measurement Of Eicosanoid Production By Lc-ms/msmentioning
confidence: 99%
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