Development and validation of a simple isocratic HPLC method for simultaneous estimation of phytosterols in Cissus quadrangularis

Shah, Unnati M; Patel, Shwetak; Patel, PH; Hingorani, Lal; Jadhav, RB

doi:10.4103/0250-474x.84587

Cited by 28 publications

(6 citation statements)

References 9 publications

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“…A variety of chromatographic methods have been used to characterize and quantify plant sterols isolated from different samples, including gas chromatography (GC) [22,[118][119][120]127,128,137,138], thin-layer chromatography (TLC) [23,131,139], and highperformance liquid chromatography (HPLC) [39,122,[140][141][142][143]. In addition to classical methods, Fourier transform infrared spectroscopy (FT-NIST), based on previously known reference values obtained by conventional methods, has been introduced for the rapid analysis of total and individual sterols; for a review, see [144][145][146].…”

Section: Determination Methodsmentioning

confidence: 99%

The Lack of Standardization and Pharmacological Effect Limits the Potential Clinical Usefulness of Phytosterols in Benign Prostatic Hyperplasia

Buț

Jîtcă

Imre

et al. 2023

Plants

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The prevalence of benign prostatic hyperplasia (BPH) markedly increases with age. Phytotherapeutic approaches have been developed over time owing to the adverse side effects of conventional medications such as 5-reductase inhibitors and α1-adrenergic receptor antagonists. Therefore, dietary supplements (DS) containing active compounds that benefit BPH are widely available. Phytosterols (PSs) are well recognized for their role in maintaining blood cholesterol levels; however, their potential in BPH treatment remains unexplored. This review aims to provide a general overview of the available data regarding the clinical evidence and a good understanding of the detailed pharmacological roles of PSs-induced activities at a molecular level in BPH. Furthermore, we will explore the authenticity of PSs content in DS used by patients with BPH compared to the current legislation and appropriate analytical methods for tracking DS containing PSs. The results showed that PSs might be a useful pharmacological treatment option for men with mild to moderate BPH, but the lack of standardized extracts linked with the regulation of DS containing PSs and experimental evidence to elucidate the mechanisms of action limit the use of PSs in BPH. Moreover, the results suggest multiple research directions in this field.

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Section: Determination Methodsmentioning

confidence: 99%

The Lack of Standardization and Pharmacological Effect Limits the Potential Clinical Usefulness of Phytosterols in Benign Prostatic Hyperplasia

Buț

Jîtcă

Imre

et al. 2023

Plants

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“…HPLC is suitable for the analysis of sensitive phytosterols. If the extracted lipids are in simple form, the phytosterols can be analyzed directly by HPLC without losing the sample 15 .…”

Section: High-performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Untitled

2024

IJPSR

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Phytosterols, a subclass of steroids, have gained considerable attention in scientific research and nutrition due to their potential health benefits. This article presents an overview of phytosterols, encompassing their sources, chemistry, extraction methods, analytical techniques, and biological activities. Phytosterols, structurally resembling cholesterol, are found abundantly in plants, animals, and fungi. Their physiological roles include influencing membrane fluidity and acting as second messengers in signal transduction pathways. Incorporating phytosterol-rich foods into the diet has been linked to various health benefits, particularly in reducing cholesterol levels, as recognized by regulatory bodies like the European Food Safety Authority and the FDA. The article discusses various extraction methods for phytosterols, emphasizing the need for environmentally friendly approaches to minimize the use of organic solvents. Traditional techniques such as Soxhlet extraction are effective but pose health and environmental concerns, driving the exploration of alternative extraction technologies. Phytosterol chemistry, characterized by specific carbon atoms and side chains, is explored, highlighting their steroid structure and different forms in nature. Analytical methods such as gas chromatography (GC), high-performance liquid chromatography (HPLC), and mass spectrometry (MS) are discussed for identifying and quantifying phytosterols, with HPLC-MS being particularly sensitive and selective. The article concludes with an examination of the diverse biological activities of phytosterols, including antiinflammatory, antimicrobial, and anti-cancer effects. The potential therapeutic applications of prominent phytosterols like β-sitosterol are also explored. Overall, this article provides a comprehensive overview of phytosterols, emphasizing their importance in nutrition and health, and the need for sustainable extraction methods to maximize their benefits. INTRODUCTION:The term "phytochemicals" (phytochemicals) was introduced to the world in 1994 and has become a distinction and boundary for scientists and researchers where phytosterols, a subclass of steroids, are important working classes of bioorganic molecules.

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“…For instance, high-performance liquid chromatography (HPLC) and UFLC methods have been established for the estimation of Quercetin [16][17][18][19][20][21][22], whereas HPLC and HPTLC techniques have been used for the estimation of Berberine [23][24][25][26][27][28][29][30][31][32][33][34], alone or in combination. Similarly, HPLC methods have been utilized for the quantification of Phytosterols [35][36][37][38][39]. These methods are important in assessing the concentration and purity of these bioactive compounds, which can be used in the formulation of nutraceuticals, functional foods, and pharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

LC method for the quantification of Quercetin, Berberine, and Phytosterol in lyophilized liposome

Bhavsar,

Jha,

Patel

et al. 2023

Separation Science Plus

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The objective of this study was to create a liquid chromatographic method that could accurately and simultaneously quantify the amount of Quercetin, Berberine, and Phytosterol present in lyophilized liposome used to treat metabolic syndrome. To achieve this goal, the team utilized a Shim‐pack GIST C8 column with a gradient elution of 0.1% (V/V) triethylamine buffer: acetonitrile at 30°C. They analyzed the phytoconstituents using an eluent flow rate of 1 mL/min and ultraviolet detection at 205 nm. The method was linear within a range of 25–77 μg/mL, which had excellent correlation coefficients (R2) of 0.9998, 0.9998, and 0.9996 for Quercetin, Berberine, and Phytosterol, respectively. The %RSD for precision and robustness of the method were also less than 2%, and the recovery values for Quercetin, Berberine, and Phytosterol were 99.5%, 100.5%, and 99.8%, respectively. The assay values of were found to be 100.2%, 101.6%, and 100.5% for Quercetin, Berberine, and Phytosterol, loaded lyophilized liposomes, respectively. As a result, this specific, accurate, and precise method can be employed for the routine analysis of Quercetin, Berberine, and Phytosterols in QC laboratories.

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Development and validation of a simple isocratic HPLC method for simultaneous estimation of phytosterols in Cissus quadrangularis

Cited by 28 publications

References 9 publications

The Lack of Standardization and Pharmacological Effect Limits the Potential Clinical Usefulness of Phytosterols in Benign Prostatic Hyperplasia

The Lack of Standardization and Pharmacological Effect Limits the Potential Clinical Usefulness of Phytosterols in Benign Prostatic Hyperplasia

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LC method for the quantification of Quercetin, Berberine, and Phytosterol in lyophilized liposome

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