Development and validation of a confirmatory method for the determination of sulphonamides in milk by liquid chromatography with diode array detection

Gamba, Valentina; Terzano, Claudio; Fioroni, Laura; Moretti, Simone; Dusi, Guglielmo; Galarini, Roberta

doi:10.1016/j.aca.2008.09.022

Cited by 46 publications

(28 citation statements)

References 23 publications

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“…Nevertheless, for official control analysis of feed samples, LC coupled with UV detection represents a valid alternative, providing good results in terms of selectivity, sensitivity, instrument cost, and simplicity. To the best of our knowledge, there are a limited number of papers about determination of sulfonamides by LC coupled with UV-diode array detection [25][26][27][28][29][30]. These methods are applied to analysis of matrices different from animal feeds and do not allow simultaneous determination of several sulfonamides in feeds.…”

Section: Introductionmentioning

confidence: 99%

Optimization and Validation of a Confirmatory Method for Determination of Ten Sulfonamides in Feeds by LC and UV-Diode Array Detection

et al. 2011

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A simple, rapid, and reliable multiresidue method, based on liquid chromatography (LC) and ultraviolet (UV)-diode array detection (DAD), is described for assaying ten sulfonamides (sulfadiazine, sulfathiazole, sulfamerazine, sulfamethazine, sulfamethoxypyridazine, sulfamonomethoxine, sulfachloropyridazine, sulfamethoxazole, sulfaquinoxaline, and sulfadimethoxine) in feeds. The chromatographic separation is accomplished using a C18 column, eluted with a mobile phase consisting of acetate buffer, acetonitrile, and methanol. The sample preparation requires a simple extraction with chloroform/ acetone and a purification step by solid-phase extraction. The analytical parameters of precision, detection and quantification limits, recovery, and ruggedness have been evaluated by a validation procedure following the European guidelines of Regulation 882/2004/EC and Decision 657/2002/EC.

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Section: Introductionmentioning

confidence: 99%

Optimization and Validation of a Confirmatory Method for Determination of Ten Sulfonamides in Feeds by LC and UV-Diode Array Detection

et al. 2011

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“…Many methods for analysis of antimicrobial agents in food such as sulfonamides, tetracyclines, penicillins have been reported (Cui et al, 2006;Gamba et al, 2009;Hermo, Nemutlu, Kr, Barrón, & Barbosa, 2008;Hoof et al, 2005;Koesukwiwat, Jayanta, & Leepipatpiboon, 2007a;Koesukwiwat et al, 2007b;Li, Wu, Yang, & Zhang, 2010;Marazuela & MorenoBondi, 2004;Ramírez et al, 2003;Volmer, 1996). The tendency nowadays is to develop multi-class methods that permit analysis for a variety of drugs with a single procedure.…”

Section: Introductionmentioning

confidence: 98%

Development of a multi-class method for the identification and quantification of residues of antibiotics, coccidiostats and corticosteroids in milk by liquid chromatography–tandem mass spectrometry

Nebot¹,

Iglesias²,

Regal³

et al. 2012

International Dairy Journal

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“…In many instances, LLE and SPE were used in combination; for example, after deproteinization/analyte extraction by means of a suitable organic solvent and solvent removal, the extracts were subsequently purified using suitable SPE procedures (Biswas et al, 2007;Soto-Chinchilla, García-Campaña, Gamiz-Gracia, 2007;Gamba et al, 2009). Procedures based on matrix solid-phase dispersion have been proposed to simplify the extraction step (Kishida, Furusawa, 2001;Bogialli et al, 2003;Posyniak, Zmudzki, Mitrowska, 2005).…”

Section: Introductionmentioning

confidence: 99%

QuEChERS-HPLC-DAD method for sulphonamides in chicken breast

Machado

Landin-Silva

Maia

et al. 2013

Braz. J. Pharm. Sci.

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The development of a QuEChERS-HPLC-DAD method using a Lichrospher 60 RP-Select B column (250 x 4.6 mm x 5 µm) at 40°C, mobile phase constituted by phosphate buffer:acetonitrile (75:25, v/v) at a initial flow rate of 0.5 mL min -1 , increased by 1.2 mL min -1 and at 265 nm is presented for simultaneous determination of sulphadiazine, sulphametoxipiridazine and sulphamethoxazole in chicken breast samples. QuEchERS is inexpensive, fast and easy, and the extraction of the analytes of the matrix was successfully employed. In addition, the method presented linearity, in the range of 25, 50, 100, 150, 175, and 200 µg kg -1 , precision, selectivity and sensitivity. The intraday precision (RSD %) for QuEChERS method was between 3.6-10.8 (SDZ), 6.9-14.1 (SPZ) and 1.9-10.9 (SMX) and interday precision (RSD%) was between 1.5-9.7, 1.7-4.1 and 2.1-10.2, respectively. Results of accuracy (bias) were in the range of -8.6 to +11.9 %. Therefore, the validated method is clearly useful for the practical residue monitoring of the drugs evaluated in chicken samples, as all the values were within the acceptable criteria used for food safety. Of 6 samples analyzed, none of them showed contamination of the sulphonamides studied at detectable levels. , aumentando 1,2 mL min -1 e a 265 nm é apresentado para a determinação simultânea de sulfadiazina, sulfametoxipiridazina e sulfametoxazol em amostras de peito de frango. O QuEChERS é barato, rápido e fácil, e a extração dos analitos da matriz foi empregada com sucesso. Além disso, o método apresentou linearidade, na faixa de 25, 50, 100, 150, 175 e 200 µg kg -1 , precisão, seletividade e sensibilidade. A precisão intradia (RSD %) para o método QuEChERS foi entre 3,6-10,8 (SDZ), 6,9-14,1 (SPZ) e 1,9-10,9 (SMX) e a precisão interdias (RSD%) foi entre 1,5-9,7, 1,7-4,1 e 2,1-10,1, respectivamente. Resultados de exatidão (tendenciosidade) foram na faixa de -8,6 a +11,9%. Portanto, o método validado é útil para a monitorização de resíduos de medicamentos avaliados em amostras de frangos, bem como todos os valores estavam dentro dos critérios aceitáveis utilizados para a segurança dos alimentos. De seis amostras analisadas, nenhuma apresentou contaminação de sulfonamidas nos níveis detectáveis estudados.

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Development and validation of a confirmatory method for the determination of sulphonamides in milk by liquid chromatography with diode array detection

Cited by 46 publications

References 23 publications

Optimization and Validation of a Confirmatory Method for Determination of Ten Sulfonamides in Feeds by LC and UV-Diode Array Detection

Optimization and Validation of a Confirmatory Method for Determination of Ten Sulfonamides in Feeds by LC and UV-Diode Array Detection

Development of a multi-class method for the identification and quantification of residues of antibiotics, coccidiostats and corticosteroids in milk by liquid chromatography–tandem mass spectrometry

QuEChERS-HPLC-DAD method for sulphonamides in chicken breast

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