Development and validation of a method for the simultaneous extraction and separate measurement of oxytetracycline, florfenicol, oxolinic acid and flumequine from marine sediments

Norambuena, Luis; Gras, N.; Contreras, Sergio

doi:10.1016/j.marpolbul.2013.05.027

Cited by 24 publications

(17 citation statements)

References 27 publications

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“…No analytes were detected in the blank extraction of the matrix samples (Figure 3). In a case of surface waters, these results are similar to those reported, where both antibiotics were not detected in marine sediments 6 . Regarding wastewater, other types of fluoroquinolones were found 30 .…”

Section: Analytical Features and Applications To Actual Samplessupporting

confidence: 91%

“…These polar drugs are highly soluble in water and are easily transferred to other types of waters 5 . In aquaculture, particularly in salmon farming, antibiotic agents are mainly administered orally in feed 6 . Lai and Lin 2009, showed that the main factor for the degradation of oxolinic acid (OXO) and flumequine (FLU) in pond water and sediment was the natural light, but this degradation is blocked in a darkness condition 7 .…”

Section: Introductionmentioning

confidence: 99%

“…Chilean salmon farmers have been battling with intracellular bacterial pathogens, so they have been forced to use a significant amount of antibiotics 6 . Therefore, an environmental point of view, it is necessary to know the situation regarding antibiotic residues in surface water of salmon farming.…”

Section: Introductionmentioning

confidence: 99%

“…The potential impact of releasing high quantities of these substances has been suggested but not proven by collateral effects, such as quantitative and qualitative modifications of bacterial flora or impacts on the food chain 6 . Thus, developing reliable analytical methods for determining antibiotic agent residues at low concentrations in aquatic systems is of critical importance.…”

Section: Introductionmentioning

confidence: 99%

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New Approach for Determination of Flumequine and Oxolinic Acid in Aqueous Samples Byionic Liquid-Based Dispersive Liquid-Liquid Microextraction and High-Performance Liquid Chromatography-Fluorescence Detection

Toledo‐Neira

Bravo

Álvarez‐Lueje

2016

J. Chil. Chem. Soc.

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A rapid and sensitive analytical method for the determination of oxolinic acid and flumequine in aqueous samples based on dispersive liquid-liquid microextraction using ionic liquids (ILs) was developed. Based on the structural properties of the antibiotic agents studied, two ILs with different functionalities were required: ethyl-dimethyl-(2-methoxyethyl) ammonium tris(pentafluoroethyl)trifluorophosphate ([MOEDEA][FAP]) for extraction and 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM][BF 4 ]) to adjust the polarity of the medium. The significant experimental factors involved in DLLME were identified and optimized using the experimental design methodology. A Plackett-Burman design was initially used for screening, and a central composite design was used for optimization. The optimized method exhibited good precision, with relative standard deviation values of less than 5 % and limits of detections on the order of 0.1 and 0.3 ng mL -1 for the two drugs. The enrichment factors for both antibiotics were 13-33 fold. The proposed method was applied to the analysis of the two antibiotic in spiked surface, river, and wastewater samples.

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Section: Analytical Features and Applications To Actual Samplessupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

New Approach for Determination of Flumequine and Oxolinic Acid in Aqueous Samples Byionic Liquid-Based Dispersive Liquid-Liquid Microextraction and High-Performance Liquid Chromatography-Fluorescence Detection

Toledo‐Neira

Bravo

Álvarez‐Lueje

2016

J. Chil. Chem. Soc.

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“…Florfenicol and diclazuril solid dispersions were prepared according to the method described by Dirikolu et al and Norambuena et al with PEG6000 as carriers [13][14][15]. After drug content test, equivalent 5 g florfenicol and 0.5 g diclazuril were transferred to a mechanical blender, followed by mixing at least 10 minutes.…”

Section: Preparation Of Florfenicol/diclazurilmentioning

confidence: 99%

Simultaneous Determination of Florfenicol and Diclazuril in Compound Powder by RP-HPLC-UV Method

Guo

Tian

Shan

et al. 2014

Journal of Chemistry

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A RP-HPLC-UV method was developed and validated for simultaneous determination of florfenicol and diclazuril in compound powder. The separation involved using a SinoChoom ODS-BP C 18 (5 m, 4.6 mm × 250 mm) analytical column. The mobile phase was a mixture of acetonitrile-0.2% phosphoric acid (pH was adjusted to 3.0 with triethylamine). The ratio of acetonitrile and 0.2% phosphoric acid in the mobile phase was 60 : 40 (v/v) from 0 minutes to 6 minutes and 70 : 30 (v/v) from 6.1 minutes to 15 minutes. The flow rate was 1 mL/min. The temperature of the analytical column was maintained at 30 ∘ C. The detection was monitored at 225 nm and 277 nm for florfenicol and diclazuril, respectively. The excipients in the compound powder did not interfere with the drug peaks. The calibration curves of florfenicol and diclazuril were fairly linear over the concentration ranges between 50.0-500.0 g/mL ( = 0.9995) and 10.0-100.0 g/mL ( = 0.9992), respectively. The RSD of both the intraday and interday variations was below 2.1% for florfenicol and diclazuril. The method was successfully validated according to International Conference on Harmonisation and proved to be suitable for the simultaneous determination of florfenicol and diclazuril in compound powder.

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Pharmaceuticals

Paszkiewicz¹,

Lis²,

Caban³

et al. 2022

Analytical Methods for Environmental Contaminants of Emerging Concern

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Development and validation of a method for the simultaneous extraction and separate measurement of oxytetracycline, florfenicol, oxolinic acid and flumequine from marine sediments

Cited by 24 publications

References 27 publications

New Approach for Determination of Flumequine and Oxolinic Acid in Aqueous Samples Byionic Liquid-Based Dispersive Liquid-Liquid Microextraction and High-Performance Liquid Chromatography-Fluorescence Detection

New Approach for Determination of Flumequine and Oxolinic Acid in Aqueous Samples Byionic Liquid-Based Dispersive Liquid-Liquid Microextraction and High-Performance Liquid Chromatography-Fluorescence Detection

Simultaneous Determination of Florfenicol and Diclazuril in Compound Powder by RP-HPLC-UV Method

Pharmaceuticals

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