Research-validated multiple-choice questions comprise an easy-to-implement instructional tool for scaffolding student learning and providing formative assessment of students’ knowledge. We present findings from the implementation of a research-validated multiple-choice question sequence on the basics of two-state quantum systems, including inner products, outer products, translation between Dirac notation and matrix representation in a particular basis, and change of basis. This study was conducted in an advanced undergraduate quantum mechanics course, in both online and in-person learning environments, across three years. Student learning was assessed after traditional lecture-based instruction in relevant concepts, and their performance was compared with that on a similar assessment given after engaging with the multiple-choice question sequence. We analyze, compare, and discuss the trends observed in the three implementations.