Development and validation of a three‐step detection protocol for broad mites (<i><scp>P</scp>olyphagotarsonemus latus</i>) in pot azalea (<i><scp>R</scp>hododendron simsii</i> hybrids)

Mechant, Els; Luypaert, Gil; Delsen, Bart Van; Pauwels, Eric J.; Witters, Johan; Huylenbroeck, Johan Van; Gobin, Bruno

doi:10.1111/eea.12315

Cited by 5 publications

(6 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our direct comparison of extraction methods showed that storing in ethanol and triplewashing leaves or crowns resulted in recovery of 5 and 14 times more cyclamen mites, on average, than using Berlese funnels did. Similarly, broad mite recovery was about six times greater from samples shaken in ethanol than from those placed in Berlese-Tullgren funnels (Mechant et al 2015). Extracting mites with Berlese funnels required less processing time than triple-washing samples did, and funnels have been used successfully for evaluating controlled atmospheric temperature-treatment effects on cyclamen mite (van Kruistum et al 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Berlese funnels have also been used to extract cyclamen mites from leaf and crown samples into ethanol, eliminating the need to mechanically separate mites from plant material (Fried and Schell 2000;van Kruistum et al 2015). Other small mites have been sampled from plant material using various techniques, including Tullgren funnels and water and sucrose (LeFors et al 2018), shaking in ethanol or water (Mechant et al 2015), or washing and sieving with detergent or ethanol (de Lillo 2001;Rezende et al 2012). Broad mite, Polyphagotarsonemus latus (Banks) (Acari: Tarsonemidae), recovery from experimentally infested rhododendron, Rhododendron simsii Planchon (Ericaceae), was greater using a method where shoot tips were shaken in ethanol or water compared to those placed in Berlese-Tullgren funnels (Mechant et al 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Other small mites have been sampled from plant material using various techniques, including Tullgren funnels and water and sucrose (LeFors et al 2018), shaking in ethanol or water (Mechant et al 2015), or washing and sieving with detergent or ethanol (de Lillo 2001;Rezende et al 2012). Broad mite, Polyphagotarsonemus latus (Banks) (Acari: Tarsonemidae), recovery from experimentally infested rhododendron, Rhododendron simsii Planchon (Ericaceae), was greater using a method where shoot tips were shaken in ethanol or water compared to those placed in Berlese-Tullgren funnels (Mechant et al 2015). To our knowledge, sampling techniques for cyclamen mite from strawberry or other host plants have not been compared.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The temporal distribution of cyclamen mite, Phytonemus pallidus (Acari: Tarsonemidae), in strawberry and comparison of sampling methods

2022

View full text Add to dashboard Cite

Cyclamen mite, Phytonemus pallidus (Banks) (Acari: Tarsonemidae), is a pest of strawberry, Fragaria × ananassa Duchesne (Rosaceae), that injures new growth, resulting in damage to leaves and fruit. The primary source of cyclamen mite in strawberry is infested planting material, and damaged plants tend to occur in small, isolated patches in perennial production systems. In the present study, we determined through repeated sampling of strawberry plants in Ontario and Saskatchewan, Canada whether relative cyclamen mite population levels were stable in commercial fields and whether overwintering and field renovation (mowing) affected populations. In Ontario, cyclamen mite counts were significantly correlated for 66% (10 of 15) of the paired comparisons from six sampling dates in more than one year, indicating a relatively stable distribution of cyclamen mite patches in strawberry fields. Winter caused high mortality (100 times fewer mites in spring than fall) and mowing caused some mortality (10 times fewer mites post- than pre-mow). We found that more cyclamen mite motiles were extracted from plant material stored in ethanol and triple-washed than were extracted using Berlese funnels, although counts from leaves were correlated between methods. No cyclamen mites were found in Saskatchewan. Knowing that cyclamen mite patches are local and spread is negligible will facilitate targeted applications of controls.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The temporal distribution of cyclamen mite, Phytonemus pallidus (Acari: Tarsonemidae), in strawberry and comparison of sampling methods

2022

View full text Add to dashboard Cite

show abstract

“…The leaflets were soaked in alcohol and then filtered with a vacuum filtration set-up. 29 Mites were counted on the filter with a stereomicroscope (Leica M125 C; Wetzlar, Germany). The count of the higher and lower parts of the plants showed that mites were found almost exclusively on the lower part, therefore the analyses proceeded with the sum of both parts as one sample from the whole plant.…”

Section: Monitoringmentioning

confidence: 99%

The predatory mite Pronematus ubiquitus curbs Aculops lycopersici damage under greenhouse conditions

Maret,

Wäckers,

Pijnakker

et al. 2023

Pest Management Science

View full text Add to dashboard Cite

BackgroundThe tomato russet mite, Aculops lycopersici, is a major worldwide pest infesting tomato crops for which only few control methods are available. At present, no commercialized beneficial organism has proven to be an effective biological control agent of the pest. As there is a strong need to develop alternatives to synthetic insecticides, we assessed the efficacy of an iolinid mite, Pronematus ubiquitus, as a preventive method against A. lycopersici in comparison with a curative treatment in a replicated experiment in the greenhouse.ResultsAfter pre‐establishment of P. ubiquitus supplied with cattail pollen, followed by infestation of A. lycopersici, the predator was able to reduce pest populations by 98% as compared with control plants. Probably due to lack of food and high temperature, the number of P. ubiquitus decreased during the season and so the Eriophyid population rose, along with crop damage. The sulphur treatment could stop the progress of A. lycopersici, but their population levels remained high.ConclusionPronematus ubiquitus has great potential to prevent the establishment of the tomato russet mite. Even if a curative treatment affects the pest mite, the use of a preventive method is preferable as such insecticides/acaricides are harmful for beneficials and are applied after symptom appearance, when the pest pressure is already high. Despite the need to optimise management of the predator throughout the season, P. ubiquitus proved to be able to establish successfully on tomato plants. © 2023 Society of Chemical Industry.

show abstract

“…Five independent biological replicates were taken at each time point (n = 5), with one shoot tip per replicate for LC-MS/MS analysis and another for mite counts. The samples for LC-MS/MS analysis were flash-frozen in liquid nitrogen and stored until analysis at −80 • C. The broad mites were extracted from the sampled shoots following the three-step detection method including (1) sampling of shoot tips, (2) isolation of the mites in 70% ethanol and (3) sieving and vacuum filtration to facilitate counting [26]. Subsequently, the mites were counted using a binocular microscope (Olympus SZX16, Antwerp, Belgium; max 115×).…”

Section: Plant Materials and Samplingmentioning

confidence: 99%

Jasmonic Acid and Salicylic Acid Levels in Defense Response of Azalea (Rhododendron simsii Hybrid) to Broad Mite (Polyphagotarsonemus latus)

et al. 2022

Self Cite

View full text Add to dashboard Cite

The broad mite (Polyphagotarsonemus latus (Banks)) is an important pest in many crops, including azalea (Rhododendron simsii Planch. hybrid). Broad mites cause the malformation of shoot tips, leaves and flowers in azalea. It is known that the jasmonic acid (JA)- and salicylic acid (SA)-dependent signaling pathways are related to the presence of herbivorous mites. Here, we describe the levels of the two main plant defense-related hormones, SA and JA, in mite-infested plants. The plant hormones were analyzed using liquid chromatography combined with tandem mass spectrometry (LC-MS/MS). We studied both short-term hormonal responses under controlled conditions with artificial inoculation, and long-term responses under culture conditions with natural infestation. The long-term development of broad mite populations and hormone response were studied during two subsequent growing seasons on 3 and 18 different cultivars, respectively. During the experiments on 18 azalea cultivars under natural infestation, the presence of different species of tarsonemid mites was also examined. JA concentrations only showed variation in the early phase of infestation. Subsequently, the SA levels increased significantly for all the cultivars where broad mites were detected. Based on the observed timing of the defense responses, we suggest that the interaction of the JA and SA pathways as a defense response for pot azalea against P. latus involves a primary plant response through the JA pathway. In the presence of the mites, the production of SA increased in the plants in a later phase as the P. latus population grew. Our results also show that the hormone response depends on type of mite. Changes in hormone levels were found upon infestation with P. latus, but not in the presence of another frequently occurring tarsonemid mite, Tarsonemus confusus Ewing.

show abstract

Development and validation of a three‐step detection protocol for broad mites (Polyphagotarsonemus latus) in pot azalea (Rhododendron simsii hybrids)

Cited by 5 publications

References 12 publications

The temporal distribution of cyclamen mite, Phytonemus pallidus (Acari: Tarsonemidae), in strawberry and comparison of sampling methods

The temporal distribution of cyclamen mite, Phytonemus pallidus (Acari: Tarsonemidae), in strawberry and comparison of sampling methods

The predatory mite Pronematus ubiquitus curbs Aculops lycopersici damage under greenhouse conditions

Jasmonic Acid and Salicylic Acid Levels in Defense Response of Azalea (Rhododendron simsii Hybrid) to Broad Mite (Polyphagotarsonemus latus)

Contact Info

Product

Resources

About