Development and validation of a liquid chromatography/tandem mass spectrometry method for determination of caspofungin in dried blood spots

Cheng, Xiaoliang; Liu, Kun-Hong; Liu, Yong; Wang, Maoyi; Ma, Ying

doi:10.1002/rcm.8100

Cited by 8 publications

(5 citation statements)

References 25 publications

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“…The selection of an appropriate method for extracting analytes from DBS is of great importance in the method development due to the significant impact of adopted extraction method on analytes recovery and the consequent sensitivity as well as the precision and accuracy. In the present study water was applied to extract tigecycline and 9‐amino minocycline which were highly hydrophilic from DBS, but the dissolution of endogenous components of blood in extraction solution led to increased interference, 21 and after extraction perchloric acid was added to the solution to precipitate protein. Matrix effect results demonstrated that extraction with water and subsequent protein precipitation with perchloric acid had no evident matrix effect for tigecycline and 9‐amino minocycline.…”

Section: Resultsmentioning

confidence: 99%

“…Extraction efficiency and matrix effect for 9-amino minocycline were 103.10% ± 6.47% and 96.97% ± 6.76%, respectively. In published analytical methods for DBSs, internal standard is added to the extraction solvents, 21,22 but the recoveries did not reflect the extraction efficiency of internal standard from DBS. In this study internal standard was pre-spotted onto filter paper, and the extraction efficiency represented recovery of it during DBS sample pretreatment.…”

Section: Precision and Accuracymentioning

confidence: 99%

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Measurement of tigecycline in dried blood spots by LC–MS/MS and comparison tigecycline concentrations between whole blood and plasma

Yan

et al. 2022

Rapid Comm Mass Spectrometry

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Rationale An LC–MS/MS method was established to measure tigecycline in dried blood spots (DBSs). Methods The DBS specimens obtained by applying 30 μl of blood to filter paper were extracted with hydrogen oxide and subsequently precipitated protein with perchloric acid, then the extract was directly analyzed by liquid chromatography tandem mass spectrometry. A Hypersil GOLD aQ column was utilized for separating the analytes, and detection was carried out in positive and selective reaction monitoring modes. The precursors to product ion transitions m/z 586.3 → 513.1 and m/z 586.3 → 569.2 were monitored for tigecycline, and m/z 473.2 → 456.0 and m/z 473.2 → 367.0 for 9‐amino minocycline as internal standard. Results The validation parameters of specificity and selectivity, linearity (0.02–5 μg ml−1), sensitivity (limit of quantification 0.02 μg ml−1), intra‐ and interday precision (within 15%) and relative error (within ±15%) were acceptable. The recoveries were from 84.65% to 90.49% and from 85.41% to 95.72% for tigecycline and internal standard, respectively, and the matrix effect was not evident to influence accuracy. The impact of hematocrit on measurement of the analyte was negligible, and after preserving at ambient temperature for 24 h and at 4°C for 1 month it remained steady. Conclusions The advantages of nonintrusive blood collection and micro‐volume sample requirements make DBS a potent surrogate to conventional venepuncture for sampling.

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Section: Resultsmentioning

confidence: 99%

Section: Precision and Accuracymentioning

confidence: 99%

Measurement of tigecycline in dried blood spots by LC–MS/MS and comparison tigecycline concentrations between whole blood and plasma

Yan

et al. 2022

Rapid Comm Mass Spectrometry

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Section: Methodsmentioning

confidence: 99%

“…The established methods were validated according to standard guidelines used for method validation in many published studies for confirming the specificity, linearity, accuracy and precision, recovery, and matrix effect in bioanalytical methods. [18][19][20][21][22] The intra-day precision (n = 5 for each QC sample, each individually prepared and analyzed) was evaluated for rat plasma on the same day, and the inter-day precision was evaluated on three consecutive days.…”

Section: Methods Validationmentioning

confidence: 99%

Quantitative determination of proxalutamide in rat plasma and tissues using liquid chromatography/tandem mass spectrometry

Sang

Wang

Zhong

et al. 2020

Rapid Comm Mass Spectrometry

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RationaleProxalutamide is a novel drug for the treatment of prostate cancer. However, to date, there are almost no reports on the pharmacokinetics of proxalutamide in vivo. This study developed a liquid chromatography/tandem mass spectrometry (LC/MS/MS) method to determine the concentrations of proxalutamide in biological samples for pharmacokinetic studies.MethodsChromatographic separation was achieved on a Kromasil 100‐5C8 column followed by gradient elution using a Shimadzu HPLC system. MS was performed in positive ion electrospray ionization mode using a SCIEX API 4000 triple quadrupole system. A simple and rapid one‐step protein precipitation method was used for sample processing, and a low sample volume of 10 μL was used for processing and analysis.ResultsThe method was validated to show good selectivity, sensitivity, precision, and accuracy. Good linearity (r2 > 0.99) was observed for rat plasma (range: 2–5000 ng/mL) and rat tissue homogenates (range: 2–2000 ng/mL). The extraction recovery was above 98%, and no significant matrix effect was observed. This method was successfully applied to investigate the pharmacokinetics and tissue distribution of proxalutamide in rats.ConclusionsA rapid and sensitive LC/MS/MS method was developed and validated to determine the quantity of proxalutamide in rat plasma and tissue homogenates and to further study the pharmacokinetic parameters of proxalutamide in a rat model. The results showed that proxalutamide had good oral bioavailability and wide tissue distribution in vivo.

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“…Recent advances in instrument sensitivity have led to the use of microsamples, and dried blood spots (DBS) are now widely used. 47,48 A VAMS (volumetric absorptive microsampling) or capillary volumetric blood microsampling assay was recently used for analyses of VRZ and VRZ N-oxide. 49 In this technique, a sampler tip is used to collect small, precise, and accurate volumes of blood (10 μL).…”

Section: Analyticsmentioning

confidence: 99%

Antifungal Drugs TDM: Trends and Update

Kably

Launay

Derobertmasure

et al. 2022

Therapeutic Drug Monitoring

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The increasing burden of invasive fungal infections results in growing challenges to antifungal (AF) therapeutic drug monitoring (TDM). This review aims to provide an overview of recent advances in AF TDM. Methods:We conducted a PubMed search for articles during 2016-2020 using "TDM" or "pharmacokinetics" or "drug-druginteraction" with "antifungal," consolidated for each AF. Selection was limited to English language articles with human data on drug exposure.Results: More than 1000 articles matched the search terms. We selected 566 publications. The latest findings tend to confirm previous observations in real-life clinical settings. The pharmacokinetic variability related to special populations is not specific but must be considered. AF benefit-to-risk ratio, drug-drug interaction (DDI) profiles, and minimal inhibitory concentrations for pathogens must be known to manage at-risk situations and patients. Itraconazole has replaced ketoconazole in healthy volunteers DDI studies. Physiologically based pharmacokinetic modeling is widely used to assess metabolic azole DDI. AF prophylactic use was studied more for Aspergillus spp. and Mucorales in oncohematology and solid organ transplantation than for Candida (already studied). Emergence of central nervous system infection and severe infections in immunocompetent individuals both merit special attention. TDM is more challenging for azoles than amphotericin B and echinocandins. Fewer TDM requirements exist for fluconazole and isavuconazole (ISZ); however, ISZ is frequently used in clinical situations in which TDM is recommended. Voriconazole remains the most challenging of the AF, with toxicity limiting high-dose treatments. Moreover, alternative treatments (posaconazole tablets, ISZ) are now available.Conclusions: TDM seems to be crucial for curative and/or longterm maintenance treatment in highly variable patients. TDM poses fewer cost issues than the drugs themselves or subsequent treatment issues. The integration of clinical pharmacology into multidisciplinary management is now increasingly seen as a part of patient care.

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Development and validation of a liquid chromatography/tandem mass spectrometry method for determination of caspofungin in dried blood spots

Abstract: The proposed method presents an alternative to the conventional venous sampling method, and was successfully utilized for pharmacokinetics study of caspofungin in ICU patients.

Cited by 8 publications

References 25 publications

Measurement of tigecycline in dried blood spots by LC–MS/MS and comparison tigecycline concentrations between whole blood and plasma

Measurement of tigecycline in dried blood spots by LC–MS/MS and comparison tigecycline concentrations between whole blood and plasma

Quantitative determination of proxalutamide in rat plasma and tissues using liquid chromatography/tandem mass spectrometry

Antifungal Drugs TDM: Trends and Update

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