Development and validation of a gas chromatography with a flame ionization detector method for quantitative analysis of four major fatty acids extracted shark liver oil

Abstract

Context: The shark liver of the species Ginglimostoma cirratun, Carcharhinus longimanus, and Carcharhinus falciformis, captured in the north-central coast of Cuba are a source of oil, whose content of major fatty acids could be used in its quality control. Aims: To develop a simple and robust gas chromatography with a flame ionization detector (GC-FID) method that is suitable for routine analysis of four major fatty acids extracted shark liver oil. Methods: Four major fatty acid content in shark liver oil pool of species Ginglimostoma cirratun, Carcharhinus longimanus, and Carcharhinus falciformis, was analyzed through the gas chromatography with a GC-FID. The fatty acids were analyzed as methyl esters derivatives, using 5% aqueous sulfuric acid in methanol. The method was validated in terms of linearity, precision, accuracy, specificity and limit of detection and quantitation. Results: Under the optimum analytical conditions, the analysis revealed that each target component was well separated with satisfactory recoveries and reproducibility. The method linearity was found to be high with good determination coefficient values for all target components. The evaluation of the matrix effect, demonstrated, that there is not interference from substances other than analysis. The method was also found to be accurate, precise and reproducible and it was applied to the quantitative determination of the fatty acid content in shark liver oil pool; oleic acid was the most abundant fatty acid (22.69%), followed by palmitic (18.85%), stearic (6.01 %) and myristic acid (0.40 %). Conclusions: The GC-FID developed method is reliable and suitable for determination of four major fatty acids in shark liver oil pool.

Local Cuban bentonite clay as potential low-cost adsorbent for shark liver oil pool purification

“…A second centrifugation was performed at 7500 rpm in the oil that was released from the heavy fats and other impurities; these were clean and transparent with a characteristic light-yellow color. The physicochemical characterization of the extracted oil was reported previously (Quero-Jiménez et al, 2020;2021a).…”

Simultaneous determination of 22 fatty acids in total parenteral nutrition (TPN) components by gas chromatography-mass spectrometry (GC-MS)

“…Moreover, establishing an appropriate method for analysing total parenteral nutrition (TPN) products, administered mostly for critically ill patients is crucial due to the chemical complexity of its components, particularly in lipid content. 1,2 For instance, analytical techniques in analysing fatty acids (FAs) in food and pharmaceutical products has evolved through the recent years from using gas chromatography-flame ionization detector (GC-FID) methods 3–9 to gas chromatography-mass spectrometry (GC-MS) methods 10–19 due to the higher sensitivity of the MS detectors, 20,21 giving a better estimate of FAs present in the analyte of interest.…”