Development and validation of a forensic six‐dye multiplex assay with 29 STR loci

Abstract

This paper describes the development and validation of a novel 31‐locus, six‐dye STR multiplex system, which is designed to meet the needs of the rapidly growing Chinese forensic database. This new assay combines 20 extended‐CODIS core loci (D3S1358, D5S818, TPOX, CSF1PO, TH01, vWA, D7S820, D21S11, D8S1179, D18S51, D16S539, D13S317, FGA, D1S1656, D2S441, D2S1338, D10S1248, D12S391, D19S433, and D22S1045), nine highly polymorphic loci in Chinese Han population (D3S3045, D6S1043, D6S477, D8S1132, D10S1435, D15S659, D19S253, Penta D, and Penta E), and two gender determining markers, amelogenin and Y‐Indel, which could amplify DNA from extracts, as well as direct amplification from substrates. To demonstrate the suitability for forensic applications, this system was validated by precision and accuracy evaluation, concordance tests, case sample tests, sensitivity, species specificity, stability, stutter calculation, and DNA mixtures, according to the guidelines described by the Scientific Working Group on DNA Analysis Methods (SWGDAM) and regulations published by the China Ministry of Public Security. The validation results indicate the robustness and reliability of this new system, and it could be a potentially helpful tool for human identification and paternity testing in the Chinese population, as well as facilitating global forensic DNA data sharing.

A sequence-based 163plex microhaplotype assay for forensic DNA analysis

“…The applicability of forensic identification was confirmed by statistical assessments. On the premise of HWE and LD compliance, the CPD of 48 MH loci was 1–8.26 × 10–44, higher than the CPD of 29 A-STR loci among the Chinese population (1–4.45 × 10–35) ( Qu et al, 2021 ).…”

Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022

“… Validation studies; concordance with GlobalFiler Harrel et al, 2021 [ 228 ] Investigator 24plex QS and GO! Assessment of sample quality metrics in both kits Jiang et al, 2021a [ 229 ] STRscan-17LC kit Validation studies (SWGDAM) Jiang et al, 2021b [ 230 ] Novel 8-dye STR multiplex Validation studies (SWGDAM); 18 STRs plus AMEL; detection with GA118-24B Genetic Analyzer Lenz et al, 2020 [ 231 ] VersaPlex 27PY system Developmental validation studies (SWGDAM); includes D6S1043 Li et al, 2021 [ 232 ] SureID S6 system Validation studies (SWGDAM); concordance with Huaxia Platinum kit; uses lyophilized reagents Liu et al, 2019 [ 233 ] 19 autosomal and 27 Y-STRs Validation studies (Chinese National Standard); 47 loci (Fusion 6C, GlobalFiler, Yfiler Plus) with 6-dyes Qu et al, 2019 [ 234 ] Microreader 20A ID system Developmental validation studies (SWGDAM) Qu et al, 2021 [ 235 ] Novel 6-dye, 31-plex Developmental validation studies (SWGDAM and Chinese National Standard); 29 STRs, AMEL, Y-InDel Wang et al, 2020a [ 236 ] 21plex with DYS391 and ABO Describes a 21plex with 18 autosomal STRs, ABO blood group locus, DYS391, and AMEL Wang et al, 2020b [ 237 ] Investigator 26plex QS kit Validation studies (SWGDAM); concordance with AGCU Expressmarker 22 kit Xie et al, 2020 [ 238 ] AGCU Expressmarker 16 + 22Y Developmental validation studies (SWGDAM) Xie et a...…”

Insight into forensic efficiency and genetic structure of the Guizhou Dong group via a 64-plex panel

“…In forensic research, forensic geneticists commonly need to solve two dominant tasks: personal identification and kinship testing. STRs are viewed as the mainstream genetic markers for forensic researchers because of their high genetic diversities and discrimination power (Fan et al, 2021;Harrel et al, 2021;Qu et al, 2021). Nonetheless, there are still some defects for STRs in forensic practice.…”