Development and validation of a PCR-based marker assay for negative selection of the HMW glutenin allele Glu-B1-1d (Bx-6) in wheat

Schwarz, Gerhard; Felsenstein, F. G.; Wenzel, G.

doi:10.1007/s00122-004-1718-5

Cited by 39 publications

(17 citation statements)

References 29 publications

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“…The panel of test cultivars was also screened with the co‐dominant primer pair 5 (PP5, Table ) specific for the Bx6 gene, developed by Schwarz et al. () on the basis of a 15‐bp insertion observed for the Bx6 allele and absent in all other Bx alleles. They reported that the PP4 profile consists of two bands of 246 bp for Bx6 cultivars and 231 bp for non‐ Bx6 cultivars (Figure e).…”

Section: Resultsmentioning

confidence: 99%

Survey and new insights in the application of PCR‐based molecular markers for identification of HMW‐GS at the Glu‐B1 locus in durum and bread wheat

et al. 2017

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Wheat, among all cereal grains, possesses unique characteristics conferred by gluten; in particular, high molecular weight glutenin subunits (HMW‐GS) are of considerable interest as they strictly relate to bread‐making quality and contribute to strengthening and stabilizing dough. Thus, the identification of allelic composition, in particular at the Glu‐B1 locus, is very important to wheat quality improvement. Several PCR‐based molecular markers to tag‐specific HMW glutenin genes encoding Bx and By subunits have been developed in recent years. This study provides a survey of the molecular markers developed for the HMW‐GS at the Glu‐B1 locus. In addition, a selection of molecular markers was tested on 31 durum and bread wheat cultivars containing the By8, By16, By9, Bx17, Bx6, Bx14 and Bx17 Glu‐B1 alleles, and a new assignation was defined for the ZSBy9_aF1/R3 molecular marker that was specific for the By20 allele. We believe the results constitute a practical guide for results that might be achieved by these molecular markers on populations and cultivars with high variability at the Glu‐B1 locus.

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Section: Resultsmentioning

confidence: 99%

Survey and new insights in the application of PCR‐based molecular markers for identification of HMW‐GS at the Glu‐B1 locus in durum and bread wheat

et al. 2017

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“…They are encoded by the Glu-1 and Glu-3 loci, respectively, on wheat homoeologous group 1 chromosomes, and play important roles in determining dough quality as they confer viscoelasticity and extensibility properties required for mixing and baking performance in common wheat (Luo et al 2001). According to the nucleotide sequences of cloned genes, functional markers were developed to identify alleles for HMW-GS and LMW-GS in wheat cultivars (D'Ovidio and Anderson 1994;Smith et al 1994;Ma et al 2003;Schwarz et al 2004;Lei et al 2006;Ishikawa and Nakamura 2007;Liu et al 2008b;Ragupathy et al 2008;Wang et al 2009bWang et al , 2010; Sup- Table 1). Markers for Glu-A3 alleles were based on DNA polymorphisms identified between the LMW glutenin genes (Zhang et al 2004), and markers developed by Wang et al (2009bWang et al ( , 2010 are much easier to use.…”

Section: Functional Markers For Processing Quality Traitsmentioning

confidence: 99%

Functional markers in wheat: current status and future prospects

et al. 2012

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Functional markers (FM) are developed from sequence polymorphisms present in allelic variants of a functional gene at a locus. FMs accurately discriminate alleles of a targeted gene, and are ideal molecular markers for marker-assisted selection in wheat breeding. In this paper, we summarize FMs developed and used in common wheat. To date, more than 30 wheat loci associated with processing quality, agronomic traits, and disease resistance, have been cloned, and 97 FMs were developed to identify 93 alleles based on the sequences of those genes. A general approach is described for isolation of wheat genes and development of FMs based on in silico cloning and comparative genomics. The divergence of DNA sequences of different alleles that affect gene function is summarized. In addition, 14 molecular markers specific for alien genes introduced from common wheat relatives were also described. This paper provides updated information on all FMs and gene-specific STS markers developed so far in wheat and should facilitate their application in wheat breeding programs.

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“…Evidences showed that Bx7?Bx8, Bx7?By9, Bx17?By18, and Bx13?By16 are correlated with favourable bread-making qualities, while subunits Bx20 and Bx6?By8 are associated with poor flour technological performances (Lawrence et al 1988;Shewry et al 2003a, b;Schwarz et al 2004;Pang and Zhang 2008). However, the diversities and variations of HMW-GS are relatively low in common wheat and only a few HMW-GS genes that have positive effects on gluten qualities are cloned so far (Gianibelli et al 2001;Jin et al 2012).…”

Section: Discussionmentioning

confidence: 96%

Isolation and molecular characterization of two novel HMW-GS genes from Chinese wheat (Triticum aestivum L.) landrace Banjiemang

et al. 2014

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High molecular weight glutenin subunits (HMW-GS) play a significant role in determining breadmaking qualities of common wheat flour. The study presented in the paper identified two novel HMW-GS (designated as 1Bx14* and 1By15*) at the Glu-B1 locus in Chinese wheat (Triticum aestivum L.) landrace Banjiemang by SDS-PAGE. The open reading frames (ORFs) of the genes were amplified and cloned with designed specific primers. Both of them had sequences highly similar to those of other HMW-GS genes, but displayed minor modification. The ORF of 1Bx14* was 2367 bp long and encoded 789 aa with 4 cysteines, which included 25 nanopeptides, 65 hexapeptides and 7 tripeptides motifs. The ORF of 1By15* was 2106 bp long and encoded 702 aa with 7 cysteines, which included 23 nanopeptides and 53 hexapeptides motifs. The predicted molecular weights of the mature proteins encoded by 1Bx14* and 1By15* genes were 83.1 and 73.2 k Da, respectively. The presence and authenticity of the two genes in Banjiemang were confirmed by bacterial expression and liquid chromatographyelectrospray ionization mass spectrometry (LC-ESI-MS). By phylogenetic analysis, the 1Bx14* was clustered with x-type subunits but had a high divergence with other 1Bx subunits, whereas the 1By15* was clustered with y-type subunits and closely related to subunit 1By15. The study concluded that 1Bx14* and 1By15* from Banjiemang were novel allelic variations of HMW-GS at Glu-B1 locus, which were probably exploitable as new resources for wheat quality improvement.

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Development and validation of a PCR-based marker assay for negative selection of the HMW glutenin allele Glu-B1-1d (Bx-6) in wheat

Cited by 39 publications

References 29 publications

Survey and new insights in the application of PCR‐based molecular markers for identification of HMW‐GS at the Glu‐B1 locus in durum and bread wheat

Survey and new insights in the application of PCR‐based molecular markers for identification of HMW‐GS at the Glu‐B1 locus in durum and bread wheat

Functional markers in wheat: current status and future prospects

Isolation and molecular characterization of two novel HMW-GS genes from Chinese wheat (Triticum aestivum L.) landrace Banjiemang

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