Development and validation of a rapid and sensitive LC-MS/MS approach for alkaloid testing in different Lupinus species

Khedr, Tawfeek; Juhász, Angéla; Singh, Karam B.; Foley, Rhonda C.; Nye-Wood, Mitchell G.; Colgrave, Michelle L.

doi:10.1016/j.jfca.2023.105391

Cited by 6 publications

(3 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…products or seeds. Historically, the quantitation of QAs has relied on chromatographic methods, which, although effective, entail prolonged analysis times and extensive extraction or pretreatment procedures [7,[28][29][30][31][32][33][34][35][36][37]39]. Analytical methods based on capillary high resolution gas chromatography have been in widespread use since the early 1980s, with minimal evolution over time.…”

Section: Discussionmentioning

confidence: 99%

“…Most articles about the analysis and quantitation of QAs use gas chromatogra equipped with a flame ionization detection (GC-FID) [28,29] or mass spectrometry MS) [7,[30][31][32][33][34], while HPLC application for that purpose is extremely limited [35][36][37] instance, a GC-MS based method was developed from Resta et al using lupanine a ternal standard in samples of lupin-based products [31] and from Romeo et al using s teine [33]. The quantitation of Lupinus alkaloids is complicated by the fact that comme standards are not easily available.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Quantitation of Lupinus spp. Quinolizidine Alkaloids by qNMR and Accelerated Debittering with a Resin-Based Protocol

Madelou,

Melliou,

Magiatis

2024

Molecules

View full text Add to dashboard Cite

Quinolizidine alkaloids (QAs) are toxic secondary metabolites of the Lupinus species, the presence of which limits the expansion of lupin beans consumption, despite their high protein content. Evaluation of the level of alkaloids in edible Lupinus species is crucial from a food safety point of view. However, quantitation of QAs is complicated by the fact that not all important alkaloids used for quantitation are commercially available. In this context, we developed a method for the simultaneous quantitation of eight major lupin alkaloids using quantitative NMR spectroscopy (qNMR). Quantitation and analysis were performed in 15 different seed extracts of 11 Lupinus spp. some of which belonged to the same species, with different geographical origins and time of harvest, as well as in all aerial parts of L. pilosus. The mature seeds of L. pilosus were found to be a uniquely rich source of multiflorine. Additionally, we developed a protocol using adsorption or ionic resins for easy, fast, and efficient debittering of the lupine seeds. The protocol was applied to L. albus, leading to a decrease of the time required for alkaloids removal as well as water consumption and to a method for QA isolation from the debittering wastewater.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quantitation of Lupinus spp. Quinolizidine Alkaloids by qNMR and Accelerated Debittering with a Resin-Based Protocol

Madelou,

Melliou,

Magiatis

2024

Molecules

View full text Add to dashboard Cite

show abstract

“…Lupine seeds have high nutrient content (protein, carbohydrates, dietary fiber, calcium, potassium, phosphorus, magnesium, iron, zinc, and vitamin C) [4]. Its glycemic index and calories are lower compared to other legumes, and it is a good source of polyphenols and zeaxanthin and contains all the essential amino acids; however, the seeds of wild lupine species have a bitter taste due to their high alkaloid content [5,6]. For this reason, in the 1920s, breeding companies focused on selecting varieties with low alkaloid contents, and as a result, recently cultivated sweet white lupine varieties were developed.…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Herbicides on Development and Productivity of Sweet White Lupine (Lupinus albus L.)

Juhász,

Mendler-Drienyovszki,

Magyar-Tábori

et al. 2024

Agronomy

View full text Add to dashboard Cite

White lupine (Lupinus albus L.) is a well-known green manure crop in Hungary, but the production of seeds can be badly impacted by weeds. The sweet white lupine ‘Nelly’ was grown on acidic sandy soil, and experimental plots were treated with different herbicides. Flumioxazin (0.06 kg ha−1), pendimethalin (5 L ha−1), dimethenamid-P (1.4 L ha−1), pethoxamid (2 L ha−1), clomazone (0.2 L ha−1), metobromuron (3 L ha−1), and metribuzin (0.55 L ha−1) were applied pre-emergence (1–2 days after sowing). Imazamox was also tested and applied post-emergence (1 L ha−1) when some basal leaves were clearly distinct (BBCH 2.3). In this paper, the weed control efficiency and the phytotoxicity of herbicides applied to lupine are examined. Vegetation index datasets were collected 12 times using a manual device and 2 times using an unmanned aerial vehicle (UAV). The phytotoxicity caused by herbicides was visually assessed on several occasions throughout the breeding season. The frequency of weed occurrence per treatment was assessed. The harvested seed yields, in kg ha−1, were analyzed after the seeds were cleaned. The herbicides metribuzin and imazamox caused extensive damage to white lupine. While pendimethalin, dimethenamid-P, pethoxamid, and clomazone were outstanding in several measured indicators, the final ranking which summarizes all the variables showed that only the pethoxamid and clomazone treatments performed better than the control. Metribuzin and imazamox were highly phytotoxic to white lupine. In the future, it would be appropriate to integrate more post-emergence active substances into trials, and the pre-emergence herbicides involved in this study should be further tested.

show abstract

The fate of quinolizidine alkaloids during the processing of lupins (Lupinus spp.) for human consumption

Schryvers

Arinzechukwu

Miserez³

et al. 2023

Food Chemistry

View full text Add to dashboard Cite

Development and validation of a rapid and sensitive LC-MS/MS approach for alkaloid testing in different Lupinus species

Cited by 6 publications

References 55 publications

Quantitation of Lupinus spp. Quinolizidine Alkaloids by qNMR and Accelerated Debittering with a Resin-Based Protocol

Quantitation of Lupinus spp. Quinolizidine Alkaloids by qNMR and Accelerated Debittering with a Resin-Based Protocol

Effect of Different Herbicides on Development and Productivity of Sweet White Lupine (Lupinus albus L.)

The fate of quinolizidine alkaloids during the processing of lupins (Lupinus spp.) for human consumption

Contact Info

Product

Resources

About