Development and validation of a gas chromatography/ion trap-mass spectrometry method for simultaneous quantification of cocaine and its metabolites benzoylecgonine and norcocaine: Application to the study of cocaine metabolism in human primary cultured renal cells

Valente, Maria João; Carvalho, Félix; Bastos, Maria de Lourdes; Carvalho, Márcia; Pinho, Paula Guedes de

doi:10.1016/j.jchromb.2010.09.010

Cited by 23 publications

(5 citation statements)

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“…Intra- and intersections reproducibility has been determined by analyzing cocaine and its metabolites in real forensic tissues samples. Data collected after the extraction from adjacent kidney sections and after normalization with the deuterated standard showed intra- and intersections reproducibility within the range 0–25% (Table S-6 in the Supporting Information), which is close to the 20% of variation reported in the literature with other techniques …”

Section: Resultssupporting

confidence: 80%

“…The sensitivity obtained with the new analytical platform is sufficient for toxicological screening of a various drugs of abuse (e.g., cocaines, opiates and opioids, amphetamines) in forensic post-mortem samples including human kidneys, of which concentrations are mainly distributed within the range of 70 to 1 540 ng per g of tissue (Table S-7 in the Supporting Information). As a comparison, typically, a validated GC/MS/MS method to study cocaine metabolism in human primary cultured renal cells could measure down to 3 ng/g . Another study reports cases where 20–100 ng/mL of cocaine have been quantified from liver and brain homogenates .…”

Section: Resultsmentioning

confidence: 99%

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Gas-Phase Separation of Drugs and Metabolites Using Modifier-Assisted Differential Ion Mobility Spectrometry Hyphenated to Liquid Extraction Surface Analysis and Mass Spectrometry

2013

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The present work describes an alternative generic approach to LC-MS for the analysis of drugs of abuse as well as their metabolites in post-mortem tissue samples. The platform integrates liquid extraction surface analysis (LESA) for analytes tissue extraction followed by differential ion mobility spectrometry (DMS) mass spectrometry for analytes gas phase separation. Detection is performed on a triple quadrupole linear ion trap using the selected reaction monitoring mode for quantification as well as product ion scan mode for structural confirmatory analyses. The major advantages of the platform are that neither chromatographic separation nor extensive sample preparation are required. In DMS the combination of a high separation voltage (i.e., up to 4 kV) together with organic modifiers (e.g., alcohols, acetonitrile, acetone) added in the drift gas is required to achieve the separation of isomeric metabolites, such as the ones of cocaine and tramadol. DMS also separates morphine from its glucuronide metabolites, which allows for preventing the overestimation of morphine in case of fragmentation of the glucuronides in the atmospheric-to-vacuum interface of the mass spectrometer. Cocaine, opiates, opioids, amphetamines, benzodiazepines and several of their metabolites could be identified in post-mortem human kidney and muscle tissue based on simultaneous screening and confirmatory analysis in data-dependent acquisition mode using an analyte-dependent compensation voltage to selectively transmit ions through the DMS cell to the mass analyzer. Quantitative performance of the LESA-DMS-MS platform was evaluated for cocaine and two of its metabolites spotted onto a tissue section using deuterated internal standard. Analyte's responses were linear from 2 to 1000 pg on tissue corresponding to a limit of detection in the order of nanograms of analyte per gram of tissue. Accuracy and precision based on QC sample was found to be less than 10%. Replicate analyses of cocaine and its metabolites in forensic samples showed an intra- and inter-sections variability of less than 25%.

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Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Gas-Phase Separation of Drugs and Metabolites Using Modifier-Assisted Differential Ion Mobility Spectrometry Hyphenated to Liquid Extraction Surface Analysis and Mass Spectrometry

2013

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“…In our laboratory, we have recently developed and validated a GC method for detection and quantification of cocaine and its metabolites in primary cultured human proximal tubular epithelial cells (HPTECs) (Valente et al, 2010). As far as we know, this was the first chromatographic technique described for the analysis of cocaine analytes in a cellular matrix.…”

Section: Gas Chromatographymentioning

confidence: 99%

Chromatographic Methodologies for Analysis of Cocaine and Its Metabolites in Biological Matrices

Valente¹,

Carvalho²,

Bastos³

et al. 2012

Gas Chromatography - Biochemicals, Narcotics and Essential Oils

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“…Existem na literatura métodos analíticos descritos para a determinação simultânea de cocaína, benzoilecgonina e AEME, empregando-se as técnicas de GC-MS (Cone et al, 1994;Jenkins & Goldberger, 1997;Kintz et al, 1997;Cardona et al, 2006;Valente et al, 2010;Fonseca Pego et al, 2017) e LC-MS/MS (Fandino et al, 2002b;Dams et al, 2003;Langman et al, 2009;Imbert et al, 2014;D'Avila et al, 2015;D'Avila et al, 2016;Dulaurent et al, 2016;Fiorentin et al, 2017a;Fiorentin et al, 2017b). Estes métodos geralmente utilizam técnicas de preparação de amostras baseadas na extração em fase sólida (SPE, do inglês "Solid Phase Extraction") ou na precipitação de proteínas (PPT, do inglês "Protein Precipitation Technique"), as quais envolvem várias etapas e podem ser encarecidas pelo custo dos cartuchos, como é o caso da SPE, ou não são capazes de remover completamente os fosfolipídios e componentes endógenos da matriz, como ocorre principalmente com a PPT (Kraemer & Paul, 2007;Barroso et al, 2009;Ismaiel et al, 2010;Jemal et al, 2010;Kole et al, 2011;Barroso & Gallardo, 2015).…”

Section: Discussionunclassified

Drogas de abuso em vítimas de mortes violentas no município de São Paulo

Takitane¹

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Aos meus pais, Luiz e Mariko, pelo amor incondicional. Essa conquista é para vocês. Aos meus irmãos, Mariana e Roberto, pelo companheirismo, amizade e carinho. Ao Thales, que surgiu na reta final deste trabalho e se tornou meu grande companheiro e incentivador. Amo você! À Professora Vilma Leyton, por ser mais do que uma orientadora. Muito obrigada pela paciência, pelo amor e pelo cuidado que tem conosco. Ao Professor Daniel Romero Muñoz pelo apoio e incentivo às pesquisas desenvolvidas no Departamento de Medicina Legal, Ética Médica e Medicina Social e do Trabalho da FMUSP. Ao meu co-orientador Gabriel Andreuccetti, por ter aceitado este papel e dividido comigo todo conhecimento. Aos amigos do Departamento de Ciências Forenses, do Hospital Universitário de Oslo, que fizeram parte de uma das experiências mais incríveis que vivenciei. Eleninha e Doffen, obrigada por me acolher com tanto carinho e dividir a família e amigos de vocês comigo. Thomas, serei eternamente grata pela paciência que teve comigo neste período e por todo aprendizado que me proporcionou. Hallvard, Vigdis e Stig Tore, obrigada por tornarem tudo aquilo possível e por me receberem tão bem. Às amigas mais "Good Vibes" que eu poderia desejar. Dani, Li (colega), Malu e Tali, a amizade de vocês é uma das coisas mais preciosas que São Paulo me deu. Amo vocês! À Dani (sim, vou repetir), porque como eu já te disse, apesar dos nossos gênios tão diferentes, você é quem me vem à mente quando algo acontece na minha vida e eu quero compartilhar. Obrigada por ser a melhor companheira de (ex-) sala! À Ana Miguel, que foi, sem dúvidas, a melhor pessoa que conheci nos últimos anos. Amiga, muito muito obrigada por ser parte da minha vida. Meu, sério, se todos tivessem uma Ana em suas vidas, as pessoas seriam infinitamente mais felizes. Sinto falta de você todos os dias, mas já sabemos que nossa amizade será para sempre. Aos amigos da minha "Família da Vida". Clari, Fer, Julitos e Rachel, vocês são, sim, os melhores amigos que o IOF poderia ter me dado. Que venham muitos cafés e Natais juntos! Aos amigos queridos do Laboratório de Toxicologia. Ana Flávia, Ju Mag(r)a, Helena, Henrique, Mari e Ana Mielli, para mim é uma felicidade enorme poder NORMALIZAÇÃO ADOTADA Esta tese está de acordo com as seguintes normas em vigor no momento desta publicação: Referências: adaptado de International Committee of Medical Journals Editors (Vancouver).

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Development and validation of a gas chromatography/ion trap-mass spectrometry method for simultaneous quantification of cocaine and its metabolites benzoylecgonine and norcocaine: Application to the study of cocaine metabolism in human primary cultured renal cells

Cited by 23 publications

References 38 publications

Gas-Phase Separation of Drugs and Metabolites Using Modifier-Assisted Differential Ion Mobility Spectrometry Hyphenated to Liquid Extraction Surface Analysis and Mass Spectrometry

Gas-Phase Separation of Drugs and Metabolites Using Modifier-Assisted Differential Ion Mobility Spectrometry Hyphenated to Liquid Extraction Surface Analysis and Mass Spectrometry

Chromatographic Methodologies for Analysis of Cocaine and Its Metabolites in Biological Matrices

Drogas de abuso em vítimas de mortes violentas no município de São Paulo

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