Development and validation of a RP-HPLC method for the simultaneous determination of Embelin, Rottlerin and Ellagic acid in Vidangadi churna

Abstract

Vidangadi churna is a popular Ayurvedic formulation described in the chapter Krimicikitsa of the Ayurvedic literature Cakradatta for the treatment of Krimiroga . The preparation is a composite mixture of the fine powder of fruits of Vidang ( Embelia ribs ), glandular trichomes of the fruits of Kamala ( Mallotus philippensis ), mature fruits of Harde ( Terminalia chebula ), Saindhava and Yavakshara. The use of reversed phase C18 column eluted with gradient mobile phase of acetonitrile and water enabled the efficient separation of the chemical markers in 22 min. Validation of the method was performed in order to demonstrate its selectivity, accuracy, precision, repeatability and recovery. All calibration curves showed good linear correlation coefficients ( r 2 >0.995) within the tested ranges. Three markers in Vidangadi churna were quantified with respect to Embelin (0.647%, w/w), Rottlerin (4.419%, w/w), and Ellagic acid (0.459%, w/w). Intra-and inter-day RSDs of retention times and peak areas were less than 3.12%. The recoveries were between 99.66% and 102.33%. In conclusion, a method has been developed for the simultaneous quantification of three markers in Vidangadi churna. The RP-HPLC method was simple, precise and accurate and can be used for the quality control of the raw materials as well as formulations.

Ancient and modern anticonvulsants act synergistically in a KCNQ potassium channel binding pocket

“…Historical medicinal usage of Mallotus plants spans West Africa ( M. oppositifolius ) and also parts of Asia, including Bangladesh, China, and India ( M. repandus , M. philippinensis , and others) 6 , 41 . Oral bioavailability of MTX in rats fed Mallotus philippensis extract was previously quantified at >20%, and plasma concentrations exceeded 2 µg/ml, reflecting also the high concentration of MTX in Mallotus ; e.g., the MTX content of powder prepared from Mallotus philippensis fruit was quantified as 21.25% w/w 42 . A plasma concentration of 2 µg/ml is equivalent to 4 µM MTX, a concentration at which we observe KCNQ2/3 activation even by MTX alone.…”

Deconstruction of an African folk medicine uncovers a novel molecular strategy for therapeutic potassium channel activation

“…In rats fed Mallotus philippensis extract, oral bioavailability of MTX has been previously quantified to be >20%, while plasma concentrations have been measured in excess of 2 μg/ml. This high concentration is aided by the high concentration of MTX in Mallotus , which was measured to be 21% (w/w) content of a powder prepared from M. philippensis fruit ( 42 ). Plasma levels (2 μg/ml) equate to 4 μM MTX, at which concentration MTX activates homomeric KCNQ1, and more efficaciously when in complexes with KCNE1.…”

Application of HPLC and ESI-MS techniques in the analysis of phenolic acids and flavonoids from green leafy vegetables (GLVs)

“…Most of the separations are carried out on reversed-phase (RP) columns [31] . RP columns used in HPLC are more desirable and widely used for the analysis of multiple phyto-constituents [41] . Routine HPLC methods use RP octadecyl silica columns for phenolic compounds [33] .…”