Development and validation of a liquid chromatography–tandem mass spectrometric method for the determination of α-methyltyrosine in human plasma

Humphries, David; Ruterbories, Kenneth J.; Chan, Choong Meng; Narayanan, R.

doi:10.1016/j.jchromb.2004.08.004

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…The same analytical column was employed for all sample analyses, but the precolumn was excluded in many methods due to problems with product consistency. The MonoChrom column was found to yield good retention of basic drugs and excellent peak shape [22]. A stainless steel frit (0.5 m) was placed before the precolumn in a stainless steel holder and changed after ca.…”

Section: Standard Gradient Methods For the Analysis Of Tamoxifen And Imentioning

confidence: 99%

Liquid chromatography–mass spectrometry/mass spectrometry method development for drug metabolism studies: Examining lipid matrix ionization effects in plasma

Little

Wempe

Buchanan

2006

Journal of Chromatography B

238

208

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Section: Standard Gradient Methods For the Analysis Of Tamoxifen And Imentioning

confidence: 99%

Liquid chromatography–mass spectrometry/mass spectrometry method development for drug metabolism studies: Examining lipid matrix ionization effects in plasma

Little

Wempe

Buchanan

2006

Journal of Chromatography B

238

208

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“…In recent years, the typical amount of plasma that bioanalytical scientists have used for their assays has dropped from 200 to 50 µL 9–13. Recent publications have suggested that there is a need to decrease the sample volume yet further 14–16. On the other hand, as new chemical entities show increased potency, lower dosage of a drug in the animals requires methods that have even higher sensitivity.…”

mentioning

confidence: 99%

Development of a low volume plasma sample precipitation procedure for liquid chromatography/tandem mass spectrometry assays used for drug discovery applications

Xu¹,

Zhou²,

Korfmacher³

2005

Rapid Comm Mass Spectrometry

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The demand for high sensitivity bioanalytical methods has dramatically increased in the drug discovery stage; in addition, there has been a growing trend of reducing the sample volume that is required for these assays. A sensitive high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) procedure has been developed and tested to meet these needs. The assay requires only a low plasma sample volume (10 microL) and employs a protein precipitation procedure using a 1:6 plasma/acetonitrile ratio. The supernatant is injected directly into the LC/MS/MS system using the selected reaction monitoring (SRM) procedure for detection. A generic HPLC gradient based on a methanol/water mobile phase with a flow rate set to 0.8 mL/min was used. The test method showed very good linearity between 0.1-1000 ng/mL (R2 = 0.9737), precision (%RSD = 6-9), accuracy (%RE = -2) and reproducibility (%RSD = 11). A drug discovery IV/PO study was assayed using both the new low volume method and our standard volume (50 microL) method. The correlation of the two sets of data from the two methods was excellent (R2 = 0.9287). This new assay procedure has been successfully used in our laboratory for over 100 different rat or mouse discovery PK studies.

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Current literature in mass spectrometry

2005

J. Mass Spectrom.

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In order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of mass spectrometry. Each bibliography is divided into 11 sections: 1 Books, Reviews & Symposia; 2 Instrumental Techniques & Methods; 3 Gas Phase Ion Chemistry; 4 Biology/Biochemistry: Amino Acids, Peptides & Proteins; Carbohydrates; Lipids; Nucleic Acids; 5 Pharmacology/Toxicology; 6 Natural Products; 7 Analysis of Organic Compounds; 8 Analysis of Inorganics/Organometallics; 9 Surface Analysis; 10 Environmental Analysis; 11 Elemental Analysis. Within each section, articles are listed in alphabetical order with respect to author (4 Weeks journals ‐ Search completed at 11th. May. 2005)

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Development and validation of a liquid chromatography–tandem mass spectrometric method for the determination of α-methyltyrosine in human plasma

Cited by 3 publications

References 18 publications

Liquid chromatography–mass spectrometry/mass spectrometry method development for drug metabolism studies: Examining lipid matrix ionization effects in plasma

Liquid chromatography–mass spectrometry/mass spectrometry method development for drug metabolism studies: Examining lipid matrix ionization effects in plasma

Development of a low volume plasma sample precipitation procedure for liquid chromatography/tandem mass spectrometry assays used for drug discovery applications

Current literature in mass spectrometry

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