Development and validation of an LC-MS/MS method with a broad linear dynamic range for the quantification of tivozanib in human and mouse plasma, mouse tissue homogenates, and culture medium

Importance of Utilizing Natural Isotopologue Transitions in Expanding the Linear Dynamic Range of LC-MS/MS Assay for Small-Molecule Pharmacokinetic Sample Analysis – A Mini-review

“…The idea of expanding the linear dynamic range of LC-MS/MS assays using isotopologue transitions were later adopted by several other researchers, for example in the quantification of methamphetamine in urine, 6 in the assessment of 80 forensically relevant compounds in 11 different postmortem sample matrices, 7 and in the quantification of tivozanib in human and mouse plasma and mouse tissue homogenates. 8 A summary of literature reported applications of multiple isotopologue transitions in expanding LC-MS/MS assay linear dynamic range is provided in Table 1.…”

Brain accumulation of tivozanib is restricted by ABCB1 (P-glycoprotein) and ABCG2 (breast cancer resistance protein) in mice

“…All other chemicals and reagents were obtained from Sigma-Aldrich (Steinheim, Germany). All chemicals used in the tivozanib assay were described before (Bruin et al 2019).…”

“…Tivozanib concentrations in cell culture medium, plasma and tissue homogenates were analyzed with a previously reported liquid-chromatography tandem mass spectrometric (LC-MS/MS) assay, using a deuterated internal standard (Bruin et al 2019).…”