Development and Validation of an Analytical Method for Nitrite and Nitrate Determination in Meat Products by Capillary Ion Chromatography (CIC)

D’Amore, Teresa; Taranto, Aurelia Di; Vita, Valeria; Berardi, Giovanna; Iammarino, Marco

doi:10.1007/s12161-019-01529-0

Cited by 30 publications

(21 citation statements)

References 24 publications

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“…This study was meant to explore GC/MS as a diagnostic tool for nitrite and nitrate analysis in meat samples, and therefore, the analyte extraction was simply adapted from consolidated methods (International Organization for Standardization, ; International Organization for Standardization, ; Della Betta et al , ; D’Amore et al , ). Since the headspace GC/MS procedure can efficiently handle complex matrices, for extraction we reduced the typical 1:10 sample:water ratio to 1:5.…”

Section: Resultsmentioning

confidence: 99%

“…A 50 g aliquot of sample was homogenised using a blade food processor. According to common practice, nitrite and nitrate are extracted from the meat matrix in hot water with (International Organization for Standardization, ; International Organization for Standardization, ; Della Betta et al , ) or without (D’Amore et al , ) borax. A 2 L volume of 0.5% aqueous borax was prepared and spiked with 10 mg L −1

^{15} {NO}_{2}^{-}

and

^{15} {NO}_{3}^{-}

isotopically enriched internal standards.…”

Section: Methodsmentioning

confidence: 99%

“…The performance of the proposed method was compared against an ion chromatography control method. Nitrite and nitrate extraction was obtained as reported by D’Amore et al , . Briefly, 4.0 g of homogenised sample was transferred in a plastic bottle with 80 mL of water.…”

Section: Methodsmentioning

confidence: 99%

“…To date, most popular methods for simultaneous determination of nitrite and nitrate are based on liquid separation. Della Betta et al , proposed an elegant capillary zone electrophoresis (CZE) method with UV detection, which could provide results within 30 s. Although CZE is a well‐suited technique for inorganic anions, CZE instruments are not widely diffused in testing laboratories and current methods for nitrite and nitrate mostly employ ion chromatography and C 18 reverse phase HPLC with conductivity (Iammarino & Di Taranto, ; Lopez‐Moreno et al , ; D’Amore et al , ), UV (Siu & Henshall, ; Hsu et al , ; Chetty et al , ) or mass spectrometry detection (Saccani et al , ; Siddiqui et al , ) although direct LC/MS methods for nitrite and nitrate are more commonly applied to simpler sample matrices (Khan et al , ; Khan et al , ). Despite such methodologies have reached a broad diffusion in food testing laboratories, liquid chromatography is typically lengthy (20–30 min per sample) and require a certain level of maintenance due to the complexity of the meat matrix.…”

Section: Introductionmentioning

confidence: 99%

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A reference isotope dilution headspace GC/MS method for the determination of nitrite and nitrate in meat samples

Luckovitch

2019

Int J of Food Sci Tech

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Summary A novel method for the determination of nitrite and nitrate in meat products is presented. The samples were ground and extracted in hot water with the presence of 15NO2- and 15NO3- internal standards. The solution was buffered with sodium bicarbonate and reacted with triethyloxonium tetrafluoroborate to convert nitrite and nitrate into EtNO2 and EtONO2. Such derivatives could be detected by headspace GC/MS in positive chemical ionisation mode with 0.05 µg g−1 NO2- and 1.0 µg g−1 NO3- LOD. The method was used for NO2- and NO3- quantitation in the 0.5‐300 and 2.5‐300 µg g−1 ranges. The method was applied for the analysis of fifteen meat products. Despite minimal sample preparation, the headspace sampling ensured a clean chromatography for over 135 analyses (throughput ten samples per hour). The proposed method offers selective GC/MS detection combined with high‐precision isotope dilution calibration, it is suitable for metrological applications and can support regulations on meat safety (European Commission, 2011).

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Section: Resultsmentioning

confidence: 99%

^{15} {NO}_{2}^{-}

and

^{15} {NO}_{3}^{-}

isotopically enriched internal standards.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A reference isotope dilution headspace GC/MS method for the determination of nitrite and nitrate in meat samples

Luckovitch

2019

Int J of Food Sci Tech

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“…The second was treated by simulating the baking in the oven (180 AE 4°C, 15 min) and then homogenised and analysed. Both portions were also analysed for the quantification of nitrite and nitrate, using a fully validated procedure by capillary ion chromatography with conductivity detection, as reported elsewhere (D'Amore et al, 2019). The N-NAs identified were quantified and related to the amounts registered of nitrite/nitrate.…”

Section: Commercial Samples and Heat Treatment Testsmentioning

confidence: 99%

Anion exchange polymeric sorbent coupled to high‐performance liquid chromatography with UV diode array detection for the determination of ten N‐nitrosamines in meat products: a validated approach

Iammarino

Mangiacotti

Chiaravalle

2019

Int J of Food Sci Tech

Self Cite

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Summary Among mechanisms and molecules presumably involved in the carcinogenicity induced by meat consumption, the N‐nitrosamines (N‐NAs) are a class of compounds characteristic of processed meats. In this work, an analytical method for the determination of ten N‐nitrosamines (N‐nitrosomorpholine, N‐nitrosomethylethylamine, N‐nitrosopyrrolidine, N‐nitrosodiethylamine, N‐nitrosopiperidine, N‐nitrosodipropylamine, N‐nitrosodimethylaniline, N‐nitrosodibutylamine, N‐nitrosodiphenylamine and N‐nitrosodibenzylamine) in fresh meats and meat products was developed, optimised and validated. The method is based on optimised sample purification by solid‐phase extraction (anion exchange polymeric sorbent) and separation/detection by high‐performance liquid chromatography coupled to UV diode array detection. The validation procedure allowed to ascertaining good analytical performances in terms of sensitivity, selectivity towards interfering compounds, accuracy and robustness. The values obtained for precision (range: 4.3–14.4%) and recovery percentages (range: 80.8–95.1%) were compared to reference values indicated in the Decision No. 657/2002/EC, resulting as compliant. The measurement uncertainty (lower than 14.6%) was satisfactory for each N‐NA as well.

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Redox-derivatization reaction-based rapid and sensitive determination of nitrite using resonance Rayleigh scattering method

Zhong

Tan

et al. 2020

Anal Bioanal Chem

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Development and Validation of an Analytical Method for Nitrite and Nitrate Determination in Meat Products by Capillary Ion Chromatography (CIC)

Cited by 30 publications

References 24 publications

A reference isotope dilution headspace GC/MS method for the determination of nitrite and nitrate in meat samples

A reference isotope dilution headspace GC/MS method for the determination of nitrite and nitrate in meat samples

Anion exchange polymeric sorbent coupled to high‐performance liquid chromatography with UV diode array detection for the determination of ten N‐nitrosamines in meat products: a validated approach

Redox-derivatization reaction-based rapid and sensitive determination of nitrite using resonance Rayleigh scattering method

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