Development and validation of an LC–MS/MS method for detection of glycyrol in rat plasma and its application to a pharmacokinetic study

Abstract

Licorice (Glycyrrhiza uralensis) is one of the most popular edible and medicinal plants and is widely used in Asia. Glycyrol (GC) is a major coumarin present in licorice that exhibits various biological activities. We aimed to develop a highly sensitive and rapid liquid chromatography coupled with mass spectrometry method for the quantitative determination analysis of GC in rat plasma. GC showed linear calibration ranges of 1–100 and 50–2,000 ng/ml with correlation coefficients >0.99. The average extraction recovery ranged from 113.26 to 114.84%, and the relative standard deviation of internal standard normalized matrix factors ranged from 6.36 to 9.46%. The intra‐day and inter‐day precisions of GC were <15%, and the accuracy ranged from 95.31 to 112.72%. Pharmacokinetic studies showed that GC was distributed in the body with a volume of distribution of 9.06 L/kg, and the initial plasma concentration was 3275.11 ng/ml. The area under the plasma concentration vs. time curve was 479.25 ng h/ml. It was rapidly eliminated with a terminal elimination half‐life of 1.47 h and a clearance rate of 4.24 L/h/kg. The pharmacokinetic results can help us to better understand the pharmacological effects of GC in the body.

Validation of a high‐performance liquid chromatography method for determining lysophosphatidylcholine content in bovine pulmonary surfactant medication

“…Subsequently, detection was carried out using a simple and cost‐effective HPLC setup that is widely available in most chromatography laboratories. Finally, owing to the importance of method validation (Beg et al, 2016; Brown, 2024; Chen et al, 2024; Chintala, 2024; Dar et al, 2022; Davis et al, 2024; Heudi & Winter, 2024; Hussien, 2014; Rajadhyaksha & Londhe, 2024; Sharma et al, 2012; Xue et al, 2012), the method underwent comprehensive validation for eight parameters. Importantly, the execution of this analytical method does not depend on any state‐of‐the‐art equipment.…”