Development and Validation of an Isocratic HPLC Method for the Simultaneous Determination of Residual Monomers Released From Dental Polymeric Materials in Artificial Saliva

Samanidou, Victoria F.; Hadjicharalampous, M.; Palaghias, Georgios; Papadoyannis, Ioannis N.

doi:10.1080/10826076.2011.601501

Cited by 17 publications

(6 citation statements)

References 22 publications

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“…However, there are studies showing that residual monomer release is negatively affected in short polymerization times [27]. 5 International Journal of Polymer Science Identification of the released components is usually carried out by using HPLC, liquid chromatography-mass spectrometry (LC-MS), and gas chromatography (GC) [32,33]. Under the normal circumstances, it is recommended to use GC for compounds that can evaporate.…”

mentioning

confidence: 99%

Effect of Polymerization Time on Residual Monomer Release in Dental Composite: In Vitro Study

Sonkaya

Kürklü

Bakır

2021

International Journal of Polymer Science

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Light activated resin-based composites are the most accepted and used materials among clinicians. The aim of this study is to determine the amount of residual monomer released from nanofiller composite resins for different polymerization times and storage periods in vitro. To this purpose, Tetric Ceram (Ivoclar, Liechtenstein), Clearfil Majesty Posterior (Kuraray, Japan), Grandio (VOCO, Germany), and Filtek Ultimate Universal (3M, USA) were used as nanofiller resin composites samples. Four groups ( n = 40 , diameter: 5 mm, thickness: 2 mm) of each material were fabricated, and each group was exposed to three different polymerization time (10, 20 and 40 sec). High-performance liquid chromatography (HPLC) was used to measure the amount of monomers released over 1, 15, and 30 days. The highest amount of monomer release was seen in Tetric EvoCream composite, while the least monomer release was seen in Clearfil Majesty composite. Regardless of the polymerization time, material, or storage period, the highest amount of eluted monomer was Bis-GMA. It is observed that there is no statistically significant difference between various polymerization times. Monomer release reached its highest level on the 15th day and decreased on the 30th day for all composites. Polymerization time did not affect the monomer release from the composites, but the type of the monomers and concentration of the filler used in the composites affected the amount of released monomers. The use of TEGDMA (co)monomer reduced the monomer release.

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confidence: 99%

Effect of Polymerization Time on Residual Monomer Release in Dental Composite: In Vitro Study

Sonkaya

Kürklü

Bakır

2021

International Journal of Polymer Science

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“…For multi-point calibration, 5-10 concentrations of each standard in the range of 0-150% or 50-150% of the concentration likely to be encountered are analyzed in duplicates or triplicates [171][172][173]. Depending on the expected eluted concentrations, most studies analyzed a series of uniformly distributed standard solutions with concentrations between 0.005 ng/mL and 1000 µg/mL [64, 89,107,120,164,[171][172][173][174][175][176]. When linear regression is used, the linearity of the calibration curve is often assessed by the correlation coefficient r or the determination coefficient r 2 [177].…”

Section: Quantitative Analysismentioning

confidence: 99%

Monomer Release from Dental Resins: The Current Status on Study Setup, Detection and Quantification for In Vitro Testing

et al. 2022

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Improvements in mechanical properties and a shift of focus towards esthetic dentistry led to the application of dental resins in various areas of dentistry. However, dental resins are not inert in the oral environment and may release monomers and other substances such as Bisphenol-A (BPA) due to incomplete polymerization and intraoral degradation. Current research shows that various monomers present cytotoxic, genotoxic, proinflammatory, and even mutagenic effects. Of these eluting substances, the elution of BPA in the oral environment is of particular interest due to its role as an endocrine disruptor. For this reason, the release of residual monomers and especially BPA from dental resins has been a cause for public concern. The assessment of patient exposure and potential health risks of dental monomers require a reliable experimental and analytical setup. However, the heterogeneous study design applied in current research hinders biocompatibility testing by impeding comparative analysis of different studies and transfer to the clinical situation. Therefore, this review aims to provide information on each step of a robust experimental and analytical in vitro setup that allows the collection of clinically relevant data and future meta-analytical evaluations.

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“…In human breast milk, BPA was extracted using Matrix Solid Phase Dispersion (LiChrolut cartridges) and reversed phase HPLC, followed with isocratic elution of a C18 column with acetonitrile-water (70:30, v / v ) [ 51 ]. The determination of the residual monomers, including BPA, released from resin-based dental restorative materials employed HPLC analysis in a Kromasil 100-C18 column eluted with methanol: acetonitrile: water, 60:15:25%, v / v [ 52 ], while the same HPLC analysis in saliva samples was performed using a Perfect Sil Target ODS-3 column eluted with acetonitrile/water, 58/42% v / v [ 53 , 54 ]. Fewer studies are engaged in the determination of multiple endocrine disruptors in complex biological samples, since extensive clean-up due to matrix interferences is required and only trace levels of the compounds are present.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Quantification of Bisphenol-A and 4-Tert-Octylphenol in the Live Aquaculture Feed Artemia franciscana and in Its Culture Medium Using HPLC-DAD

Giamaki

Dindini

Samanidou

et al. 2022

MPs

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Aquaculture, a mass supplier of seafood, relies on plastic materials that may contain the endocrine disruptors bisphenol-A (BPA) and tert-octylphenol (t-OCT). These pollutants present toxicity to Artemia, the live aquaculture feed, and are transferred through it to the larval stages of the cultured organisms. The purpose of this work is the development and validation of an analytical method to determine BPA and t-OCT in Artemia and their culture medium, using n-octylphenol as the internal standard. Extraction of the samples was performed with Η2Ο/ TFA (0.08%)–methanol (3:1), followed by SPE. Analysis was performed in a Nucleosil column with mobile phases A (95:5, v/v, 0.1% TFA in H2O: CH3CN) and B (5:95, v/v, 0.08% TFA in H2O: CH3CN). Calibration curves were constructed in the range of concentrations expected following a 24 h administration of BPA (10 μg/mL) or t-OCT (0.5 μg/mL), below their respective LC50. At the end of exposure to the pollutants, their total levels appeared reduced by about 32% for BPA and 35% for t-OCT, and this reduction could not be accounted for by photodegradation (9–19%). The developed method was validated in terms of linearity, accuracy, and precision, demonstrating the uptake of BPA and t-OCT in Artemia.

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Development and Validation of an Isocratic HPLC Method for the Simultaneous Determination of Residual Monomers Released From Dental Polymeric Materials in Artificial Saliva

Cited by 17 publications

References 22 publications

Effect of Polymerization Time on Residual Monomer Release in Dental Composite: In Vitro Study

Effect of Polymerization Time on Residual Monomer Release in Dental Composite: In Vitro Study

Monomer Release from Dental Resins: The Current Status on Study Setup, Detection and Quantification for In Vitro Testing

Simultaneous Quantification of Bisphenol-A and 4-Tert-Octylphenol in the Live Aquaculture Feed Artemia franciscana and in Its Culture Medium Using HPLC-DAD

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