Development and Validation of an Automated Chemiluminometric Immunoassay for Human Intrinsic Factor Antibodies in Serum

Abstract

served not only decreases in FA concentrations (assumingly attributable to FA peroxidation), but also increases. A second process may be ongoing during storage: the formation of erythrocyte microparticles. In vitro, glycolysis will lower the glucose concentration in erythrocytes, causing depletion of ATP. Consequently, the erythrocytes cannot maintain membrane integrity, and microparticles will be released (15 ). Over time, the concentration of plasma phospholipid-associated FAs may thus become enriched with erythrocyte membrane phospholipids containing relatively high amounts of polyunsaturated FAs.Our study has some limitations. One limitation is that we did not measure in duplicate to adjust for intraassay variation. In addition, serum samples were not analyzed within one run to minimize interassay variability. However, standardized procedures were used for analysis, with CVs Յ7.9% for serum analytes and Յ5.6% for plasma phospholipid-associated FAs. In addition, the reliability analyses showed high agreement between follow-up and baseline values. Another limitation is that samples were not randomized before analysis. Possible bias from order of draw, although unlikely, can therefore not be ruled out. A third limitation is that we were not able to test the reliability and validity of CRP over the 28-h storage period. However, changes in concentration were small (Յ5.5%), which, as mentioned before, is in line with results from other studies (3, 5 ).In conclusion, this study shows that in the context of epidemiologic studies investigating (nutritional) status during routine care, a pragmatic approach to blood collection may validly be applied to determine CRP, retinol, ferritin, folic acid, or FA status. Although storage will diminish the precision of estimates, standard (correlational) epidemiologic analyses will not be compromised in samples stored for a maximum of 28 h.

Do intrinsic factor antibodies assays provide univocal answers in Biermer's disease?

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Indicators and methods for folate, vitamin B-12, and vitamin B-6 status assessment in humans

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Vitamin B12 deficiency: New data on an old disease

“…IF-Antikörper werden immunologisch mit verschiedenen Methoden bestimmt [67]. Die Spezifität des Tests gilt als hoch, das heißt, dass ein positiver Test die Diagnose perniziöse Anämie beweist.…”