Development and validation of an LC–MS/MS procedure for environmental monitoring of eight cytostatic drugs in pharmacies

Tuerk, Jochen; Kiffmeyer, Thekla K.; Hadtstein, Claudia; Heinemann, André W.; Hahn, Moritz; Stuetzer, Hartmut; Kuß, Heinz-Martin; Eickmann, Udo

doi:10.1080/03067319.2010.494769

Cited by 27 publications

(15 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To study the impact of monitoring, 130 pharmacies were randomized into two groups.Environmental monitoring in group A was regular throughout the study period, but occurred only at the beginning and at the end of the project in group B. Using a validatedmulticompound technique (Tuerk et al 2011),eight cytotoxics were quantified, a sample being considered positive if at least one of the drugs was detected. Contamination levels were constantly decreasing in group Apharmacies, where, after the first report of results, cleaning protocols, gloves, equipment modifications and more intensive training of cytotoxic drugs' handlerwere implemented.…”

Section: Applications and Discussionmentioning

confidence: 99%

Environmental monitoring by surface sampling for cytotoxics: a review

Marie

Curti

Roche

et al. 2017

Environ Monit Assess

View full text Add to dashboard Cite

Environmental monitoring is usually conducted by surface sampling to detect and quantify the presence of cytotoxic drugs after their reconstitution and administration. This technique reveals the origins of residual contamination and is an important component in order to protect healthcare workers from the potential risk of occupational exposure. The aim of this work is to compare various techniques and results of surface sampling for cytotoxics. For each technique, sample processing methods and their analysis were compared from literature data. Sampling is often performed by the wiping technique. After treatment, various single or multicompound technical analyses are used, in particular liquid or gas chromatography involving different detection methods: ultraviolet, mass spectrometry, plasma torch, and voltammetry. Some methods are validated to ensure reliability. Despite published guidelines and the use of isolator technology for the preparation of cytotoxic drugs, workplace contamination persists, leading to chemotherapeutic agents' exposure of healthcare workers. Efforts need to be maintained with particular emphasis on harmonization and on determining alert level for cytotoxic contamination.

show abstract

Section: Applications and Discussionmentioning

confidence: 99%

Environmental monitoring by surface sampling for cytotoxics: a review

Marie

Curti

Roche

et al. 2017

Environ Monit Assess

View full text Add to dashboard Cite

show abstract

“…sampling, storage, transport, sample pretreatment and HPLC-MS/MS analysis have been developed and optimized according to the needs of MEWIP. Details of the analytical methods and the thorough validation are presented elsewhere by Tuerk et al (2011).…”

Section: Methodsmentioning

confidence: 99%

“…Table 1. LOD (signal-to-noise ratio = 3:1) and LOQ (lowest calibration level) of the HPLC-MS/MS analysis (Tuerk et al, 2011 Sampling kit and procedure All steps of the monitoring procedure [sample collection, storage, transport, sample pretreatment, high-performance liquid chromatography using mass selective detection (HPLC-MS/MS) analysis] have been optimized in order to increase sensitivity and reliability as well as throughput. Due to the large number of participants and measurements, it has been necessary to develop a specially designed kit ( Fig.…”

Section: Selection Of Sampling Spots Time and Substancesmentioning

confidence: 99%

Application and Assessment of a Regular Environmental Monitoring of the Antineoplastic Drug Contamination Level in Pharmacies - The MEWIP Project

Kiffmeyer

Tuerk

Hahn

et al. 2012

The Annals of Occupational Hygiene

View full text Add to dashboard Cite

A large-scale study was carried out in order to determine the contamination level of antineoplastic drugs in pharmacies and to investigate the suitability and effects of wipe sample monitoring at regular intervals. A specific study design was developed. The 130 participating pharmacies were divided into a study and a control group, carrying out five and two wipe sampling cycles, respectively. The work practice was analyzed using questionnaires to identify factors that influence the contamination level. From 1269 wipe samples, 774 (61%) were contaminated with at least one of the analyzed cytotoxic drugs: cyclophosphamide, docetaxel, etoposide, 5-fluorouracil, gemcitabine, ifosfamide, methotrexate, and paclitaxel. A significant decrease of the contamination with cyclophosphamide and 5-fluorouracil was observed in the study group. The Monitoring-Effect Study of Wipe Sampling in Pharmacies method has proven to be a reliable and affordable tool for contamination control. Based on the 90th percentile of the contamination values, a substance-independent performance-based guidance value of 0.1ng cm(-2) has been derived.

show abstract

“…Occupational exposure of health care workers to cytotoxic drugs has been studied intensively and has resulted in guidelines for the safe handling of these substances in many countries [9]. However, despite high safety standards traces of cytotoxic agents have been found in urine and blood of healthcare professionals [10][11], and monitoring studies in pharmacies and hospitals have revealed that contamination of the workplace occurs frequently [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

On-line solid phase extraction–liquid chromatography–tandem mass spectrometry for the determination of 17 cytostatics and metabolites in waste, surface and ground water samples

Negreira

Alda

Barceló

2013

Journal of Chromatography A

115

View full text Add to dashboard Cite

A fully automated on-line solid-phase extraction-liquid chromatography-tandem mass spectrometry (SPE-LC-MS/MS) method has been developed for the determination of 13 cytostatics and 4 metabolites in aqueous matrices, including groundwater, surface water, and raw and treated wastewater. On-line SPE is performed by loading 5 mL of water sample at pH 2 through a PLRP-s cartridge. MS/MS is performed with an electrospray (ESI) interface operating in the positive ion mode and registering two selected reaction monitoring (SRM) transitions per compound. Quantification is carried out by the isotope dilution method using 15 different isotope-labelled compounds, specific for the target analytes, as internal standards (IS). The main advantages of the method are high sensitivity, with limits of determination in groundwater, surface water, and raw and treated wastewater below 5 ng L(-1) for all compounds except for gemcitabine (6.9-9.3 ng L(-1)), temozolomide (26-50 ng L(-1)), imatinib (80-180 ng L(-1)) and etoposide (38-65 ng L(-1)), repeatability, with relative standard deviations (RSDs) in most cases below 15%, and selectivity and reliability of results. The method is also fairly simple and fast, with an analysis time per sample (excluding the manual steps, i.e., sample filtration, pH adjustment, and addition of IS) of 40 min. Application of the method to influent wastewater samples collected daily during eight consecutive days from a wastewater treatment plant (WWTP) from Catalonia showed the presence of methotrexate, ifosfamide, capecitabine, tamoxifen and 6(α)-hydroxypaclitaxel but at fairly low concentrations (up to 43 ng L(-1)).

show abstract

Development and validation of an LC–MS/MS procedure for environmental monitoring of eight cytostatic drugs in pharmacies

Cited by 27 publications

References 35 publications

Environmental monitoring by surface sampling for cytotoxics: a review

Environmental monitoring by surface sampling for cytotoxics: a review

Application and Assessment of a Regular Environmental Monitoring of the Antineoplastic Drug Contamination Level in Pharmacies - The MEWIP Project

On-line solid phase extraction–liquid chromatography–tandem mass spectrometry for the determination of 17 cytostatics and metabolites in waste, surface and ground water samples

Contact Info

Product

Resources

About