Development and Validation of Dithiocarbamates Fungicide Analytical Method using CS₂ Trap Method in Livestock Product

Abstract

BACKGROUND: Dithiocarbamte fungicides have been used in crop cultivation for diseases protection and treatment. And cultivated agricultrual products were used as feedstuff, and residual pesticides are likely to be absorbed and transferred to livestock. But the maximum residue limits (MRLs) were not established for dithiocarbate fungicides in livestock products, and thus an analysis method was developed and validated for dithiocarbamate fungicides to establish MRLs. METHODS AND RESULTS: Samples were prepared using CS 2 trap method and detected with UV/VIS spectrophotometer. Calibration line (0.1 ∼ 10 ㎍/mL) was linear with r 2 ⟩ 0.99. For validation, the recovery tests were carried out at three fortification levels (MLOQ, 10 MLOQ and 50 MLOQ) from livestock samples (egg, milk, beef, pork, and chicken). The results for mancozeb, propineb, and thiram ranged between 76.8 to 109.6%, 79.4 to 108.8%, and 80.2 to 107.8%, respectively and % RSD (relative standard deviation) values were below 9.5%. Furthermore, inter-laboratory analysis was performed to validate the method. CONCLUSION: All values were corresponded with the criteria ranges requested by both the CODEX (CAC/GL 40-199340- , 200340- ) and MFDS guidelines (2016. This might be used as an official analytical method for determination of dithiocarbamate fungicides at established MRLs and monitoring.

Improvement of Analytical Method for Propineb Residues in Glycine max (L.) Merrill and Pisum sativum L. using Deproteinization Process

“…현재 propineb를 포함하는 dithiocarbamate계 살균제의 잔류분석법으로는 CS 2 gas 포집방법과 methyl iodide (CH 3 I)를 이용한 유도체화 방법이 보고되고 있다 [6][7][8]. 가장 기본적인 CS 2 분석법은 SnCl 2 /HCl시약을 가한 후 기류를 통과시키면 100℃ 이상에서 분해되어 발생하는 CS 2 가스를 발색시약에 포집하여 흡광도를 측정하는 정량법이다 [5].…”