Development and validation of eDNA markers for the detection of Crepidula fornicata in environmental samples

Miralles, Laura; Parrondo, Marina; Rojas, Alma Hernández de; García‐Vázquez, Eva; Borrell, Yaisel J.

doi:10.1016/j.marpolbul.2019.07.050

Cited by 19 publications

(11 citation statements)

References 22 publications

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“…Detection and mapping of the spread of invasive or non-native species Invasive slipper shell on the European Atlantic coast [39] Identification and monitoring of rare/endangered species White sharks in the open ocean [34] Detection of cryptic species Cryptic seahorse species off western Australia [40] Biosecurity during import/export Ornamental fish imports [41] Investigating spawning activity Spawning ecology of the Japanese eel [42] Monitoring of hard to access environments Deep-sea octocorals using remote submersibles [43] Applications The catadromous Japanese eel Anguilla japonica is an important food fish in East Asia, where after spawning at sea and migrating to freshwater it is raised in aquaculture ponds. Intensive research including sampling with large plankton and trawl nets, genetic species identification of eggs and newly hatched larvae, and direct observations using deep-tow camera systems has led to the discovery of the eel's spawning area.…”

Section: Application Example Study Outline Examplementioning

confidence: 99%

Life in a drop: Sampling environmental DNA for marine fishery management and ecosystem monitoring

et al. 2021

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Section: Application Example Study Outline Examplementioning

confidence: 99%

Life in a drop: Sampling environmental DNA for marine fishery management and ecosystem monitoring

et al. 2021

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“…Early detection is a vital step for the effective management of invasive species. The species-specific molecular markers for C. fornicata presented by Miralles et al can be used to detect the early stages of invasions due to their sensitivity, low cost, and ease and speed of laboratory testing [142]. The results of that research demonstrate the presence of C. fornicata in close proximity to the M. gigas culture facilities operating in the Eo estuary.…”

Section: Facing Biological Invasions and Their Threat To Asturias Exp...mentioning

confidence: 85%

Sustainable Management Plans in Fisheries and Genetic Tools: An Overview of the Challenge in Invertebrates’ Fisheries at the Central Area of the Southern Bay of Biscay, Spain

Parrondo Lombardía,

García-Florez,

Dopico Rodríguez

et al. 2023

Pertinent and Traditional Approaches Towards Fishery

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The fishing and aquaculture sectors are an important source of development around the globe. In Asturias (Spain), the diversity and richness of the fishing grounds of the Cantabrian Sea favored the historical settlement of a large number of communities closely linked to the marine environment and fishing resources, forming an integral part of the region’s cultural and natural heritage. However, aquatic ecosystems are facing, nowadays, important threats from anthropogenic activities. To address these problems and avoid their impact on fishing activities, it is essential to know the ecological and genetic status of the species. Despite this, the application of genetic tools is still incipient in many species of commercial interest; however, its use can help to generate data that allow better regulation and fisheries planning. Here, the use of genetic markers and educational strategies in the management of some shellfish species of great commercial and cultural value in Asturias are reviewed. Moving toward sustainable fisheries management is a priority that can only be achieved through R + D + i, educational strategies, and the development and implementation of a regional strategy oriented toward the sustainable management and exploitation.

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“…Currently, in addition to physical surveys, environmental DNA (eDNA) assays could also be a useful alternative for determining the presence of species based on organism vestigial particles that remain in the environment, negating the need to catch or observe the organism (Darling and Mahon, 2011;Borrell et al, 2017). It is also possible to design species-specific primers for the rapid, early detection of certain species of interest within a seawater community using eDNA (e.g., Devloo-Delva et al, 2016;Miralles et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“If You Know the Enemy and Know Yourself”: Addressing the Problem of Biological Invasions in Ports Through a New NIS Invasion Threat Score, Routine Monitoring, and Preventive Action Plans

Miralles

Ibabe

González³

et al. 2021

Front. Mar. Sci.

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Invasive alien species (IAS) are currently considered one of the greatest threats to global marine ecosystems. Thus, ships and maritime activity have been identified as the main factors responsible for the vast majority of accidental species translocations around the world, implying that prevention should be the core of environmental port policies. Preventive port strategies should include analyzing risks based on traffic origins and volumes, revising port policies for inspections, estimating probabilities of non-indigenous species (NIS) appearance, monitoring routine species within ports, and finally implementing management plans and focused actions. Here, we conducted a comprehensive NIS prediction analysis for the port of Gijon (northern Spain), one of the largest ports in the south Bay of Biscay, as a case study that can be extrapolated to other international seaports. An extensive bibliographic search (1953–2020) was conducted and we identified 380 species that have been transported through hull fouling and ballast water around the world. We evaluated their likelihood of arriving (from 14 years of traffic data) and becoming established (from habitat suitability and demonstrated impacts and invasion ability) within the Gijon port, creating a new NIS Invasion Threat Score (NIS-ITS). This new index could help to identify target species that are likely invaders for early detection and prevention policies within the port. The results showed that 15 NIS had >90% likelihood of becoming a biological invasion problem in Gijon Port. At the same time, we reported morphological and genetic analysis of biota found in two successive annual monitoring surveys of Gijon port and ships (n = 612 individuals) revealing 18 NIS, including 6 of the NIS predicted from high NIS-ITS. Actually, 80% (12 NIS) of those potentially most dangerous species (NIS-ITS > 90%) have already been detected in the Bay of Biscay area. We propose the use of this new tool for a risk-reduction strategy in ports, based on accurate predictions that help in promoting specific early detection tests and specific monitoring for NIS that have a high chance of establishment. All international seaports can adopt this strategy to address the problem of biological invasions and become “blueports” in line with EU policy.

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Development and validation of eDNA markers for the detection of Crepidula fornicata in environmental samples

Cited by 19 publications

References 22 publications

Life in a drop: Sampling environmental DNA for marine fishery management and ecosystem monitoring

Life in a drop: Sampling environmental DNA for marine fishery management and ecosystem monitoring

Sustainable Management Plans in Fisheries and Genetic Tools: An Overview of the Challenge in Invertebrates’ Fisheries at the Central Area of the Southern Bay of Biscay, Spain

“If You Know the Enemy and Know Yourself”: Addressing the Problem of Biological Invasions in Ports Through a New NIS Invasion Threat Score, Routine Monitoring, and Preventive Action Plans

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