Development and Validation of Hilic–ESI/MS/MS Methods for Simultaneous Quantitation of Several Antipsychotics in Human Plasma and Blood

Meulder, Marc De; Waldron, Michael P; Li, Linge; Peay, Marlking; Tingler, Michael; Hidy, B.; Verhaeghe, Tom; Jenkins, Rand

doi:10.4155/bio.16.27

Cited by 19 publications

(24 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For patients taking paliperidone, quetiapine, or olanzapine, concentrations of the parent compound and for patients taking risperidone or aripiprazole, concentrations of the parent compound and the major metabolite (9‐hydroxyrisperidone [paliperidone] and dehydroaripiprazole, respectively) were determined in plasma using validated liquid chromatography–tandem mass spectrometry methods . In addition, for patients taking aripiprazole or risperidone, the sum of parent drug and measured metabolite was calculated (“aripiprazole active moiety” and “risperidone active moiety”).…”

Section: Methodsmentioning

confidence: 99%

Comparison of Capillary and Venous Plasma Drug Concentrations After Repeated Administration of Risperidone, Paliperidone, Quetiapine, Olanzapine, or Aripiprazole

Remmerie

Meulder

Ariyawansa

et al. 2016

Clinical Pharm in Drug Dev

View full text Add to dashboard Cite

Quantification of blood levels of antipsychotic drugs may be useful for managing medication therapy. This open‐label, parallel‐group study was performed to compare finger‐stick‐based capillary with corresponding venous plasma concentrations for risperidone, paliperidone, quetiapine, olanzapine, and aripiprazole and their major metabolites after repeated dosing in patients with schizophrenia or related illnesses. Finger‐stick‐based capillary and venous blood samples were collected at various times within a dosing interval. All drug concentration measurements in the derived plasma samples were performed with validated liquid chromatography–tandem mass spectrometry methods. Finger‐stick‐based capillary and venous plasma drug concentrations after repeated dosing were generally similar. Olanzapine capillary plasma concentrations, however, were on average approximately 20% higher than venous concentrations, with a trend for a relatively greater difference occurring shortly after dosing. In addition, smaller capillary–venous differences were observed for extended‐release and long‐acting intramuscular formulations and for aripiprazole, a drug with a long half‐life, compared with drugs administered as an immediate‐release formulation (risperidone, olanzapine). After repeated dosing, plasma derived from finger‐stick‐based blood was observed to be predictive of the venous concentrations. Capillary sampling may be an appropriate alternative to venous sampling to readily evaluate systemic drug concentrations.

show abstract

Section: Methodsmentioning

confidence: 99%

Comparison of Capillary and Venous Plasma Drug Concentrations After Repeated Administration of Risperidone, Paliperidone, Quetiapine, Olanzapine, or Aripiprazole

Remmerie

Meulder

Ariyawansa

et al. 2016

Clinical Pharm in Drug Dev

View full text Add to dashboard Cite

show abstract

“…For participants taking paliperidone, quetiapine, or olanzapine, concentrations of the parent compound, and for participants taking risperidone or aripiprazole, concentrations of the parent compound and the major metabolite (9‐hydroxyrisperidone and dehydroaripiprazole, respectively) were determined in whole blood and derived plasma, using validated liquid chromatography–tandem mass spectrometry methods . In addition, the sums of parent drug and major metabolite were calculated for participants taking risperidone (“risperidone active moiety”) or aripiprazole (“aripiprazole active moiety”).…”

Section: Methodsmentioning

confidence: 99%

“…In the venous plasma samples collected, quetiapine metabolites were determined using qualified research methods, the performance of which is described below per analyte: quetiapine sulfoxide, quetiapine sulfone, 7‐hydroxyquetiapine, norquetiapine, and O ‐desalkylquetiapine were quantified . For this qualified analytical method, the generally accepted criteria on accuracy of 15% (European Medicines Agency, 2011; Food and Drug Administration, 2001) could not be met for all metabolites.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Capillary and Venous Drug Concentrations After Administration of a Single Dose of Risperidone, Paliperidone, Quetiapine, Olanzapine, or Aripiprazole

Remmerie

Meulder

Weiner

et al. 2016

Clinical Pharm in Drug Dev

View full text Add to dashboard Cite

Risperidone, paliperidone, quetiapine, olanzapine, and aripiprazole are antipsychotic drugs approved for treating various psychiatric disorders, including schizophrenia. The objective of this randomized, parallel‐group, open‐label study was to compare finger‐stick‐based capillary with corresponding venous whole‐blood and plasma concentrations for these drugs after administration of a single dose to healthy volunteers. All whole‐blood and plasma drug concentrations were measured with validated liquid chromatography–tandem mass spectrometry methods. Capillary and venous concentrations (both in plasma and whole blood) were in close agreement, although a time‐dependent difference was observed, most obviously for olanzapine and paliperidone, with slightly higher capillary versus venous drug concentrations during the first hours after administering a single dose. The observed difference between capillary and venous plasma drug concentrations is expected not to be relevant in clinical practice, considering the wide window of therapeutic concentrations and the wide range of drug concentrations in the patient population for a given dose. Based on these results, finger‐stick‐based capillary drug concentrations have been shown to approximate venous drug concentrations.

show abstract

“…The HILIC-MS/MS method has been widely applied for the quantitative determinations of different drugs in human plasma [137][138][139][140][141][142] because it requires only a small volume of plasma, thanks to the improved sensitivity and rapid sample pretreatment, by-passing the evaporation of the organic solvent and reconstitution of the sample in a highly aqueous solvent, when the protein precipitation procedure or the Solid Phase Extraction step are performed, unlike in RPLC mode. These advantages permit routine application of the HILIC method to bioanalytical analyses [143].…”

Section: Chromatographic Conditionsmentioning

confidence: 99%

Compensate for or Minimize Matrix Effects? Strategies for Overcoming Matrix Effects in Liquid Chromatography-Mass Spectrometry Technique: A Tutorial Review

et al. 2020

View full text Add to dashboard Cite

In recent decades, mass spectrometry techniques, particularly when combined with separation methods such as high-performance liquid chromatography, have become increasingly important in pharmaceutical, bio-analytical, environmental, and food science applications because they afford high selectivity and sensitivity. However, mass spectrometry has limitations due to the matrix effects (ME), which can be particularly marked in complex mixes, when the analyte co-elutes together with other molecules, altering analysis results quantitatively. This may be detrimental during method validation, negatively affecting reproducibility, linearity, selectivity, accuracy, and sensitivity. Starting from literature and own experience, this review intends to provide a simple guideline for selecting the best operative conditions to overcome matrix effects in LC-MS techniques, to obtain the best result in the shortest time. The proposed methodology can be of benefit in different sectors, such as pharmaceutical, bio-analytical, environmental, and food sciences. Depending on the required sensitivity, analysts may minimize or compensate for ME. When sensitivity is crucial, analysis must try to minimize ME by adjusting MS parameters, chromatographic conditions, or optimizing clean-up. On the contrary, to compensate for ME analysts should have recourse to calibration approaches depending on the availability of blank matrix. When blank matrices are available, calibration can occur through isotope labeled internal standards and matrix matched calibration standards; conversely, when blank matrices are not available, calibration can be performed through isotope labeled internal standards, background subtraction, or surrogate matrices. In any case, an adjusting of MS parameters, chromatographic conditions, or a clean-up are necessary.

show abstract

Development and Validation of Hilic–ESI/MS/MS Methods for Simultaneous Quantitation of Several Antipsychotics in Human Plasma and Blood

Abstract: Robust and efficient LC-MS/MS assays were established and were effective in providing antipsychotic drug matrix comparator results in the intended clinical studies.

Cited by 19 publications

References 24 publications

Comparison of Capillary and Venous Plasma Drug Concentrations After Repeated Administration of Risperidone, Paliperidone, Quetiapine, Olanzapine, or Aripiprazole

Comparison of Capillary and Venous Plasma Drug Concentrations After Repeated Administration of Risperidone, Paliperidone, Quetiapine, Olanzapine, or Aripiprazole

Comparison of Capillary and Venous Drug Concentrations After Administration of a Single Dose of Risperidone, Paliperidone, Quetiapine, Olanzapine, or Aripiprazole

Compensate for or Minimize Matrix Effects? Strategies for Overcoming Matrix Effects in Liquid Chromatography-Mass Spectrometry Technique: A Tutorial Review

Contact Info

Product

Resources

About