Development and Validation of HLPC Method for the Estimation of Lamotrigine in Bulk and Pharmaceutical Formulations

Reddy, T. V.; Ramu, G.; Babu, A. Ramesh; Rambabu, C.

doi:10.1155/2013/846170

Cited by 4 publications

(1 citation statement)

References 22 publications

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“…The method lacks discussion on forced degradation studies and no specified impurities were discussed in specificity. Another UPLC method was reported on forced degradation study and quantification of mycophenolate in tablets [21]. This method was developed on BEH column having dimensions 2.1 mm × 100 mm, 1.7 µm.…”

Section: Lc-ms Compatible Stability Indicating Rp-uplc Methods For Thementioning

confidence: 99%

LC-MS Compatible Stability Indicating RP-UPLC Method for the Estimation of Ester Prodrug of Mycophenolic Acid in Injection Formulation

Rao¹,

Krishna²,

Bellam³

et al. 2018

Asian J. Chem.

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This study explores the ultra performance liquid chromatography for the quantification of mycophenolate mofetil (an immunosuppressant agent) in an injection formulation. Mycophenolate mofetil was separated from its seven specified impurities on HSS (high strength silica) C18 column (100 × 2.1 mm, 1.8 µm). The optimized mobile phase is compatible to mass spectrometry and hence the method can be equally run on LC-MS system for mass determinations. Efficient UV detection at 250 nm enabled determination of mycophenolate mofetil with no interference from placebo solution, diluent and other specified and unspecified impurities. The retention time of mycophenolate mofetil in the method was 1.79 min and all other impurities were eluted within in 8 min. The method optimization studies show that the selectivity of mycophenolate mofetil and its impurities was dependent on the type of organic solvent and its elution strength. The method was validated for specificity, linearity, precision, accuracy and robustness in range of 50 to 150 % of target analyte concentration (i.e. 62.50-195.31 µg mL -1 ). The linearity of peak area responses versus concentrations was demonstrated by linear least square regression analysis. An excellent linear relation exists between the concentration and detector response (R 2 > 0.9999). Precision of the test method was proved with a relative standard deviation less than 1.0 %.

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Section: Lc-ms Compatible Stability Indicating Rp-uplc Methods For Thementioning

confidence: 99%

LC-MS Compatible Stability Indicating RP-UPLC Method for the Estimation of Ester Prodrug of Mycophenolic Acid in Injection Formulation

Rao¹,

Krishna²,

Bellam³

et al. 2018

Asian J. Chem.

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Assessment of combined electro–nanoremediation of molinate contaminated soil

Gomes

Fan

Mateus

et al. 2014

Science of The Total Environment

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Molinate is a pesticide widely used, both in space and time, for weed control in rice paddies. Due to its water solubility and affinity to organic matter, it is a contaminant of concern in ground and surface waters, soils and sediments. Previous works have showed that molinate can be removed from soils through electrokinetic (EK) remediation. In this work, molinate degradation by zero valent iron nanoparticles (nZVI) was tested in soils for the first time. Soil is a highly complex matrix, and pollutant partitioning between soil and water and its degradation rates in different matrices is quite challenging. A system combining nZVI and EK was also set up in order to study the nanoparticles and molinate transport, as well as molinate degradation. Results showed that molinate could be degraded by nZVI in soils, even though the process is more time demanding and degradation percentages are lower than in an aqueous solution. This shows the importance of testing contaminant degradation, not only in aqueous solutions, but also in the soil-sorbed fraction. It was also found that soil type was the most significant factor influencing iron and molinate transport. The main advantage of the simultaneous use of both methods is the molinate degradation instead of its accumulation in the catholyte.

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Lamotrigine—A Review of Analytical Methods Developed for Pharmaceutical Formulations and Biological Matrices

Sajeeda

Kumar

Verma

2021

Critical Reviews in Analytical Chemistry

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Development and Validation of HLPC Method for the Estimation of Lamotrigine in Bulk and Pharmaceutical Formulations

Cited by 4 publications

References 22 publications

LC-MS Compatible Stability Indicating RP-UPLC Method for the Estimation of Ester Prodrug of Mycophenolic Acid in Injection Formulation

LC-MS Compatible Stability Indicating RP-UPLC Method for the Estimation of Ester Prodrug of Mycophenolic Acid in Injection Formulation

Assessment of combined electro–nanoremediation of molinate contaminated soil

Lamotrigine—A Review of Analytical Methods Developed for Pharmaceutical Formulations and Biological Matrices

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