Development and validation of HPLC method for the determination of ferrocyanide ion in food grade salts

Lim, Ho Soo; Hwang, Ju Young; Choi, Euna; Lee, Gunyoung; Yoon, Sang Sun; Kim, MeeKyung

doi:10.1016/j.foodchem.2017.07.070

Cited by 22 publications

(5 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A method developed for an HPLC procedure must accurate, sensitive, and able to identify an analyte with high selectivity. For this purpose, validation of analytical methods should include assessments of linearity, selectivity, accuracy (recovery), precision, LOD, LOQ, and measurement uncertainty [15]. In our study, the UV spectrum of the HA in the mobile phase was scanned in the region between 190 and 800 nm.…”

Section: Resultsmentioning

confidence: 99%

Development and validation of an HPLC method for the determination of hyaluronic acid active substance in pharmaceutical formulations

Çağlar¹,

Okur²,

Karasulu³

2022

jrp

View full text Add to dashboard Cite

It is an analytical requirement to identify and determine high molecular weight APIs in pharmaceutical forms. For this reason, it is aimed to develop and validate an analytical method for the determination of hyaluronic acid in pharmaceutical form in this study. 0.1 M Na2SO4 was prepared as mobile phase. The separation of compound was performed with a OHpak Shodex SB-806M HQ (13 µm, 8,0 x 300mm, Japan) analytical column. Guard column which is OHpak SB-G, (8 µm, 100 Å, 6.0 x 50 mm, Japan) was integrated to the system before the analytical column. UV detection at 198 nm was used to monitor the eluent and flow rate of the mobile phase was set to 1 mL/min. The method has been validated in terms of system suitability, linearity, limits of detection (LOD) and quantity (LOQ), precision, accuracy, specificity, selectivity, and stability. The obtained findings showed that the analytical method has linearity higher than 0.99, accuracy, precision, selectivity and stability. The method was found to be precise, accurate and specific during the study.

show abstract

Section: Resultsmentioning

confidence: 99%

Development and validation of an HPLC method for the determination of hyaluronic acid active substance in pharmaceutical formulations

Çağlar¹,

Okur²,

Karasulu³

2022

jrp

View full text Add to dashboard Cite

show abstract

“…Furthermore, there are also studies that use the same solvent for both standard and sample preparation. For instance, Lim et al [25] analyzed ferrocyanide ions using HPLC-UV by employing the same solvent (0.02 M NaOH solution) for the preparation of the standard solution and pretreatment of the sample. In addition, Klimczak and Gliszczyńska-Świgło [27] quantified vitamin C (ascorbic acid and dehydroascorbic acid) using HPLC and ultraperformance liquid chromatography (UPLC) systems by using 10% meta-phosphoric acid solvent to both extract the samples and dilute the standard solutions.…”

Section: Resultsmentioning

confidence: 99%

Quantitative Characteristics of Toxic Compounds According to the Solvent Type

Choi

Kim

et al. 2019

Journal of Analytical Methods in Chemistry

View full text Add to dashboard Cite

The quantitative analysis of target substances is an important part of assessing the toxicity of diverse materials. Usually, the quantitation of target compounds is conducted by instrumental analysis such as chromatography and capillary electrophoresis. If solvents are used in the pretreatment step of the target analyte quantification, it would be crucial to examine the solvent effect on the quantitative analysis. Therefore, in this study, we assessed the solvent effects using four different solvents (methanol, hexane, phosphate buffered saline (PBS), and dimethyl sulfoxide (DMSO)) and three toxic compounds (benzene, toluene, and methylisothiazolinone (MIT)). Liquid working standards containing the toxic compounds were prepared by dilution with each solvent and analyzed by gas chromatography-mass spectrometry (GC-MS). As a result, we found that the response factor (RF) values of the target analytes were different, depending on the solvent types. In particular, benzene and toluene exhibited their highest RF values (33,674 ng−1 and 78,604 ng−1, respectively) in hexane, while the RF value of MIT was the highest (9,067 ng−1) in PBS. Considering the correlation (R2) and relative standard deviation (RSD) values, all target analytes showed fairly good values (R2 > 0.99 and RSD < 10%) in methanol and DMSO. In contrast, low R2 (0.0562) and high RSD (10.6%) values of MIT were detected in hexane, while benzene and toluene exhibited relatively low R2 and high RSD values in PBS (mean R2 = 0.9892 ± 0.0146 and mean RSD = 13.3 ± 4.1%). Based on these findings, we concluded that the results and reliability of the quantitative analysis change depending on the analyte and solvent types. Therefore, in order to accurately assess the toxicity of target compounds, reliable analytical data should be obtained, preferentially by considering the solvent types.

show abstract

“…The FI system was successfully applied to determine ferrocyanide at mg/kg level in real salt samples with a precision better than 3% and quantitative recovery. Lim et al (2018) developed and validated a rapid high-performance liquid chromatography (HPLC) method to determine the presence of ferrocyanide ions ([Fe(CN) 6 ] 4-) in food grade salts (sodium chloride). An analytical column coupled with a guard column and mobile phase comprised of sodium perchlorate and sodium hydroxide were employed with a photodiode array detector set at a wavelength of 221 nm.…”

Section: Methods Of Analysis In Foodmentioning

confidence: 99%

Re‐evaluation of sodium ferrocyanide (E 535), potassium ferrocyanide (E 536) and calcium ferrocyanide (E 538) as food additives

Younes¹,

Aggett²,

Aguilar³

et al. 2018

EFS2

View full text Add to dashboard Cite

The Panel on Food Additives and Nutrient Sources added to Food (ANS) provided a scientific opinion re‐evaluating the safety of sodium ferrocyanide (E 535), potassium ferrocyanide (E 536), and evaluating the safety of calcium ferrocyanide (E 538) as food additives. The Panel considered that adequate exposure and toxicity data were available. Ferrocyanides (E 535–538) are solely authorised in two food categories as salt substitutes. To assess the dietary exposure to ferrocyanides (E 535–538) from their use as food additives, the exposure was calculated based on regulatory maximum level exposure assessment scenario (maximum permitted level (MPL)) and the refined exposure assessment scenario. Dietary exposure to ferrocyanides was calculated based on mean and high levels consumption of salts in both the regulatory maximum level and the refined scenario. In the MPL scenario, the exposure to ferrocyanides (E 535–538) from their use as a food additive was up to 0.009 mg/kg body weight (bw) per day in children and adolescents. In the refined estimated exposure scenario, the exposure was up to 0.003 mg/kg bw per day in children and adolescents. Absorption of ferrocyanides is low and there is no accumulation in human. There is no concern with respect to genotoxicity and carcinogenicity. Reproductive studies were not available, but a no observed adverse effect level (NOAEL) of 1,000 mg sodium ferrocyanide/kg bw per day (highest dose tested) was identified from a prenatal developmental toxicity study. The kidney appeared to be the target organ for ferrocyanides toxicity and 4.4 mg sodium ferrocyanide/kg bw per day was identified as the NOAEL for the renal effects in a chronic (2‐year) study in rats. Assuming that the toxicity of this compound is due to the ferrocyanide ion only, the Panel established a group acceptable daily intake (ADI) for sodium, potassium and calcium ferrocyanide of 0.03 mg/kg bw per day expressed as ferrocyanide ion. The Panel concluded that ferrocyanides (E 535–538) are of no safety concern at the current authorised use and use levels.

show abstract

Development and validation of HPLC method for the determination of ferrocyanide ion in food grade salts

Cited by 22 publications

References 8 publications

Development and validation of an HPLC method for the determination of hyaluronic acid active substance in pharmaceutical formulations

Development and validation of an HPLC method for the determination of hyaluronic acid active substance in pharmaceutical formulations

Quantitative Characteristics of Toxic Compounds According to the Solvent Type

Re‐evaluation of sodium ferrocyanide (E 535), potassium ferrocyanide (E 536) and calcium ferrocyanide (E 538) as food additives

Contact Info

Product

Resources

About