Development and validation of HPTLC method for simultaneous estimation of curcumin and galangin in polyherbal capsule dosage form

Abstract

Simple and precise HPTLC methods were developed for the simultaneous estimation of two anti-inflammatory drugs (curcumin and galangin). The method was tailored to analyze both drugs in their commercial dosage form (capsules) with no interference from ingredients. Chromatographic separation was performed over precoated TLC plates (60 F 254 , 20 cm × 10 cm, 250 m thickness, Merck, Darmstadt, Germany) via a linear ascending technique using n-hexane, ethyl acetate, acetic acid, and methanol as the mobile phase. Detection and quantification was achieved at 404 nm through spectro-densitometric analysis. Analytical performance of the proposed HPTLC method was validated according to the ICH guidelines with respect to the linearity, accuracy, precision, detection and quantitation limits, robustness and specificity. The calibration curves were linear with the limits of 80-450 and 200-1200 ng/spot for CU and GA, respectively, with correlation coefficients (r 2 ) >0.9998. The limits of detection were 18.31 and 40.50 ng/spot for CU and GA, respectively. The validated HPTLC method was successfully applied to the simultaneous determination of CU and GA in the commercial polyherbal formulation.

Curcumin: Biological, Pharmaceutical, Nutraceutical, and Analytical Aspects

“…The techniques used for sample analysis are primarily dependent on the instrument availability and the research objective of the study. Table 3 [33,38,39,40,41,42,43,44,45,46,47,48,49,134,135] and Table 4 [14,25,26,27,28,29,30,31,35,36,37,93,96,133,136,137,138,139,140,141,142,143,144,145] summarize the various instrumentation and methods used to study and quantify curcumin in different matrices.…”

“…It is essential to understand how different sample preparation and analysis techniques impact the quantification, bioavailability, pharmacokinetic profiles, and clinical efficacy of CCMs [25,26,27,28,29,30,31,32,33], which in turn will assist in increasing understanding of curcuminoids as a potential therapeutic agent [7,26,31,34]. This review presents current research updates in the areas of CCMs formulations, and their clinical outcomes [6,14,19,20,21], sample preparation [25,26,27,28,29,31,32,33,35,36,37,38,39], and various methodologies used to analyze CCMs [25,26,33,38,39,40,41,42,43,44,45,46,47,48,49]. Sample preparation techniques developed for extracting CCMs from plants, dietary supplements, and other biological matrices, have been described.…”

Formulation and Evaluation of Cefixime Nanosuspension for the Enhancement of Oral Bioavailability by Solvent-Antisolvent Method and its Suitable Method Development

“…The validation for HPTLC method development for optimized cefixime Nano suspension was performed using parameters like identification, linearity, precision, specificity and recovery. 18,19…”

Eco-friendly stability-indicating RP-HPTLC method for sildenafil analysis, characterization and biological evaluation of its oxidized stress degradation product

“…Another advantage of HPTLC is the higher sensitivity and capability to detect more compounds than HPLC. The colourful pattern and quantification at the micron and nanogram levels helps to compare various samples on the same plate 11 , 12 .…”