Development and validation of isomer specific RP-HPLC method for quantification of cefpodoxime proxetil

Kakumanu, Vasu Kumar; Arora, Vinod; Bansal, Arvind K.

doi:10.1016/j.jchromb.2006.02.032

Cited by 24 publications

(6 citation statements)

References 12 publications

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“…The HPLC method used for detection of CP in formulations employed acetonitrile: ammonium acetate buŠer (pH 5.0) as mobile phase at 38 : 62, pumped at a ‰ow rate of 1 ml/m, and analysis was carried at a temperature of 30°C with detection at 235 nm. 10) Similarly, the HPLC method employed for detection of CA in the in vivo samples involved pumping of acetonitrile and phosphate buŠer (pH 3.0) in 10 : 90 ratio at a ‰ow rate of 1 ml/min, and the detection was carried out at 269nm. The method employed for quantifying CP had a calibration range of 5 150 mg/ ml with a LOQ of 900 ng/ml, an accuracy of 98.45 101.63％ and intra and inter day precision values of ％ RSD of 0.95 4.29.…”

Section: Methodsmentioning

confidence: 99%

Gastro-retentive Dosage Form for Improving Bioavailability of Cefpodoxime Proxetil in Rats

2008

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Cefpodoxime proxetil (CP) is a prodrug with poor oral bioavailability because of its metabolism to Cefpodoxime acid (CA) in luminal contents and intestinal epithelial cells. In the present investigation, regional variability in diŠerent segments of the gastrointestinal tract vis-àa-vis solubility and metabolism were investigated, and the results indicated potential for a gastro retentive (GR) dosage form. Suitability of a GR dosage from for CP andˆnally in vivo e‹cacy were investigated. Thereafter, an eŠervescent ‰oating GR dosage form was developed for CP and evaluated in rats. The GR dosage form improved the oral bioavailability of CP signiˆcantly by about 75％, hence providing a proof-of-concept. The T max value increased to 1.43±0.24 h from 0.91±0.23 h of pure drug, while C max values of 4735±802 ng/ml and 3094±567 ng/ml were obtained for the GR dosage form and pure drug respectively.

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Section: Methodsmentioning

confidence: 99%

Gastro-retentive Dosage Form for Improving Bioavailability of Cefpodoxime Proxetil in Rats

2008

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“…CP is supplied as a racemic mixture of two enantiomers R and S in a ratio of about 1 : 1 [7]. There are some reported methods for the quantification of cefpodoxime proxetil alone and in combination with potassium clavulanate [10][11][12][13][14], and there are a few stability indicating assay methods and methods for related substances [15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Cefpodoxime Proxetil: A New Stability Indicating RP-HPLC Method

Mathew

Ajitha

Babu

2013

ISRN Chromatography

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The present work describes the development of a sensitive and economic stability indicating high performance liquid chromatographic (HPLC) method for the determination of cefpodoxime proxetil (CP) as bulk drug and as pharmaceutical formulation. Both R and S isomers of the drug were separated using Phenomenex (250 × 4.6 mm, 5 m particle size) ODS column with a flow rate of 1 mL min −1 and an SPD 20 A UV detector to monitor the eluate at 252 nm. The isocratic method used a mobile phase consisting of methanol and phosphate buffer of pH 4.0 in the ratio 65 : 35. The linear regression analysis data for the calibration plots showed good linear relationship with 2 = 0.9999 in the working concentration range of 5-100 g mL −1 . The LOD and LOQ were 53 and 160 ng mL −1 , respectively. CP was subjected to stress degradation using acid, alkali, hydrogen peroxide, dry heat, wet heat, and UV light. The standard drug peaks were well resolved from the degradation products' peaks with significantly different retention time (Rt), and the resolution factor for the R and S isomers of CP was found to be greater than 2.

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“…It is a metabolic product of bromhexine. Ultraviolet-visible spectrophotometric method has been reported for the quantitative determination of CEFPO from pharmaceutical formulation spectrophotometric method…[1] using high performance liquid chromatography (HPLC),[2–4] and high performance thin layer chromatography (HPTLC). [5] A method for the simultaneous determination of AMBRO has not been reported with CEFPO such as UV spectrophotometry,[6] HPTLC[7] and HPLC[8] and in human plasma using LC-MS/MS.…”

Section: Introductionmentioning

confidence: 99%

Development of HPTLC method for determination of cefpodoxime proxetil and ambroxol hydrochloride in human plasma by liquid–liquid extraction

Rote¹,

Kande²

2011

Pharmaceutical Methods

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Aim:To develop a simple, sensitive, rapid, and economic high performance thin layer chromatographic method for determination of cefpodoxime proxetil (CEFPO) and ambroxol hydrochloride (AMBRO) in human plasma by liquid–liquid extraction using paracetamol as an internal standard.Materials and Methods:The plasma sample was extracted by a mixture of methanol and acetonitrile. A concentration range from 500 to 3500 ng/spot for CEFPO and 1000 to 7000 ng/spot for AMBRO were used for the calibration curve, respectively. This recovery was found to be 74.40 and 94.50 for CEFPO and AMBRO, respectively. The mobile phase used consists of chloroform: methanol (9:1v/v). Densitometric analysis was carried out at a wavelength of 240 nm.Results:The Rf values for CEFPO, AMBRO, and paracetamol were found to be 0.69 ± 0.005, 0.49 ± 0.0057, and 0.31 ± 0.0054, respectively. The stability of CEFPO and AMBRO in plasma was confirmed during three freeze–thaw cycles (-20°C), on bench during 24 h and post preparative during 48 h.Conclusion:The proposed method was validated statistically and by performing a recovery study for determination of CEFPO and AMBRO in human plasma.

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Development and validation of isomer specific RP-HPLC method for quantification of cefpodoxime proxetil

Cited by 24 publications

References 12 publications

Gastro-retentive Dosage Form for Improving Bioavailability of Cefpodoxime Proxetil in Rats

Gastro-retentive Dosage Form for Improving Bioavailability of Cefpodoxime Proxetil in Rats

Cefpodoxime Proxetil: A New Stability Indicating RP-HPLC Method

Development of HPTLC method for determination of cefpodoxime proxetil and ambroxol hydrochloride in human plasma by liquid–liquid extraction

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