Development and validation of microarray-based serological assay for Crimean-Congo hemorrhagic fever (CCF) and determination of the prevalence of CCFV in Guinea

Abstract

classified as ZIKV seropositive and the remaining 12 (16.7%) as flavivirus seropositive. Eleven of 14 provinces contributed ZIKV positive specimens, ranging from 4.5% of specimens from North Sumatra, Banten, and East Kalimantan to more than 18% of specimens from Central Java. Overall ZIKV seroprevalence in the 1-4 year-old cohort was 9.1%.Conclusion: We have conducted ZIKV serological screening on samples collected from children in representative areas in Indonesia and revealed the seroprevalence of ZIKV in most regions. The data provide evidence of widespread recent transmission and endemicity of ZIKV in the country. This knowledge provides clues for understanding future patterns of Zika virus transmission in nonendemic areas.

Differential Laboratory Diagnosis of Acute Fever in Guinea: Preparedness for the Threat of Hemorrhagic Fevers

“…In total, 15.9% of acute febrile illnesses had bacterial etiology in our study. Similarly, DNA from at least one pathogenic bacterium were identified in 80/440 (18.2%) of the samples from febrile patients in Senegal (35,30,23, 2, and 1 cases for Borellia crocidurae, Rickettsia felis, Bartonella spp., Coxiella. burnetii, and Tropheryma whipplei identification, respectively [46].…”

“…Some previous evaluation work has been performed with the microarray [ 22 , 23 , 28 , 29 , 30 , 31 , 32 ]. Negative control samples were taken from Russian healthy donors.…”

“…For ZEBOV and MARV, samples were considered positive for the presence of anti-virus IgM if antibodies were found against their corresponding NP antigens. Some previous evaluation work has been performed with the microarray [22,23,[28][29][30][31][32]. Negative control samples were taken from Russian healthy donors.…”

Recent Advances in Crimean-Congo Hemorrhagic Fever Virus Detection, Treatment, and Vaccination: Overview of Current Status and Challenges

“…Microarray detects IgM/IgG distinctly but in one well because of the use of Cy3/Cy5-labeled particular Ab. The researchers demonstrated that the recently advanced microarray is a very accurate and specific diagnostic instrument [ 161 ].…”