Development and Validation of Microbiological Assay for Ceftriaxone and its Application in Photo-stability Study

Lourenço, Felipe Rebello; Traple, Marcus Augusto Lyrio; Okamoto, Rogerio Takao; Pinto, Terezinha de Jesus Andreoli

doi:10.2174/1573412911309010011

Cited by 1 publication

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“…Initial examination of the raw data would be mandatory to exclude any unusual record from the dataset for this initial step. This could be followed by the investigation of the sources of the variation in the testing using ANOVA [29]. Statistical analysis of the raw data should reveal a dataset pattern that would be trustworthy to proceed in further computation to study the suitability of the assay design for potency calculation of the subject material [30].…”

Section: Initial Statistical Investigation Of the Suitability Of The ...mentioning

confidence: 99%

Statistical Comparison of Parallel-Line Symmetrical Microbiological Models: Analysis of Agar Diffusion Assay in 8 x 8 Large Rectangular Plates

Eissa¹

2021

İstatistik Ve Uygulamalı Bilimler Dergisi

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(1) Background: Microbiological assay of active medicinal compounds is superior to conventional chemical means in several circumstances to date. However, ensuring the validity and suitability of the assay design proposed for the intended purpose is crucial before deriving any records or conclusions from the results of the potency determination; (2) The present work represented statistical comparison between three design models for determination of the potency of Neomycin Sulfate antibiotic using agar diffusion technique for the same test material subject under identical conditions through the application of a combination of statistical software programs, including validated programmed Microsoft Excel Workbook for the statistical testing of each assay layout; (3) Results: raw data of the three assay designs were found to be reasonably valid for further analysis of the assay suitability. Examination of the sources of variations for each design demonstrated the validity of the conducted experimentation. Variation between the computed potencies from the three designs was lower than 5 µg/mg. However, there was significant variation between the confidence windows of each type; (4) Conclusions: 2 x 4 design had the narrowest confidence range. However, improving confidence would require investigation of the assay parameters, including the modification of the number of replicates per treatment.

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Section: Initial Statistical Investigation Of the Suitability Of The ...mentioning

confidence: 99%

Statistical Comparison of Parallel-Line Symmetrical Microbiological Models: Analysis of Agar Diffusion Assay in 8 x 8 Large Rectangular Plates

Eissa¹

2021

İstatistik Ve Uygulamalı Bilimler Dergisi

View full text Add to dashboard Cite

show abstract

Development and Validation of Microbiological Assay for Ceftriaxone and its Application in Photo-stability Study

Cited by 1 publication

References 3 publications

Statistical Comparison of Parallel-Line Symmetrical Microbiological Models: Analysis of Agar Diffusion Assay in 8 x 8 Large Rectangular Plates

Statistical Comparison of Parallel-Line Symmetrical Microbiological Models: Analysis of Agar Diffusion Assay in 8 x 8 Large Rectangular Plates

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