Development and Validation of RP-HPLC Method for Simultaneous Estimation of Moexipril and Hydrochlorothiazide in Bulk and Tablet Dosage Form

Abstract

A simple, fast, precise reverse phase and isocratic HPLC method was developed for the separation and quantification of Moexipril (MXP) and Hydrochlorothiazide (HCT) in pharmaceutical dosage form. The quantification was carried out using Kromasil C18 (250 x 4.6 mm, 5 µm) enhanced polar selectivity column and mobile phase comprised of orthophosphoric acid buffer and acetonitrile in proportion of ratio 70:30v/v and degassed under ultra sonication. The flow rate was 1.0mL/min and the effluent was monitored at 230nm. The retention time of Moexipril and Hydrochlorothiazide were 2.4 and 3.2min respectively. The method was validated in terms of linearity, precision, accuracy, specificity, limit of detection and limit of quantitation. Linearity of Moexipril and Hydrochlorothiazide were in the range of 37.5 to 225µg/mL and 62.5 to 375µg/mL respectively. The percentage recoveries of both the drugs were 98.44% and 98.80% for Moexipril and Hydrochlorothiazide respectively from the tablet formulation. The proposed method is suitable for simultaneous determination of Moexipril and Hydrochlorothiazide in pharmaceutical dosage form.