Development and validation of sex-linked molecular markers for rapid and accurate identification of male and female Hippophae tibetana plants

Zeng, Zhefei; Wang, Ruoqiu; Wang, Junwei; Chen, Yonghao; Wang, Yuguo; Song, Zhiping; Zhang, Wenju; Qiong, La

doi:10.1038/s41598-024-69918-y

Sci Rep

2024

DOI: 10.1038/s41598-024-69918-y

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Development and validation of sex-linked molecular markers for rapid and accurate identification of male and female Hippophae tibetana plants

Zhefei Zeng,

Ruoqiu Wang,

Junwei Wang

et al.

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Development of sex-specific molecular markers for early sex identification in Hippophae gyantsensis based on whole-genome resequencing

Zeng,

Wang,

Tian

et al. 2024

BMC Plant Biol

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Hippophae gyantsensis is a dioecious plant endemic to the Qinghai-Tibet Plateau and is significant for ecological restoration and sand stabilization. Its fruit is rich in bioactive compounds that offer economic potential. However, the inability to distinguish sexes before flowering and prolonged maturation hinder breeding and cultivation. We performed whole-genome resequencing on male and female plants, identified large insertion/deletion (InDel) variants, and developed two sex-specific primers (Higy_04 and Higy_06). These primers enable rapid, accurate PCR-based sex identification. All sex-specific sites were located on chromosome 2, suggesting its potential role as the sex chromosome. Additionally, we found a 1:1 sex ratio among offspring from the same mother plant, consistent with Mendelian inheritance, indicating that sex segregation is mainly genetically controlled. This work lays the foundation for developing molecular markers applicable across the entire genus Hippophae and contributes to understanding sex chromosome formation and adaptive evolution within the genus. Supplementary Information The online version contains supplementary material available at 10.1186/s12870-024-05978-6.

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Development of sex-specific molecular markers for early sex identification in Hippophae gyantsensis based on whole-genome resequencing

Zeng,

Wang,

Tian

et al. 2024

BMC Plant Biol

View full text Add to dashboard Cite

show abstract

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Development and validation of sex-linked molecular markers for rapid and accurate identification of male and female Hippophae tibetana plants

Cited by 1 publication

References 37 publications

Development of sex-specific molecular markers for early sex identification in Hippophae gyantsensis based on whole-genome resequencing

Development of sex-specific molecular markers for early sex identification in Hippophae gyantsensis based on whole-genome resequencing

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