Development and validation of the stability indicating RP-UHPLC method for the determination of the chemical purity and assay of bimatoprost

Spanlastic-laden nanogel as a plausible platform for dermal delivery of bimatoprost with superior cutaneous deposition and hair regrowth efficiency in androgenic alopecia

“…Finally, skin samples were collected, cleaned with phosphate buffer, and then homogenized with methanol to extract BIM that remained in the skin. Skin homogenate was then centrifuged at 10000 rpm for 15 min and BIM content in the supernatant was quantified by HPLC analysis as previously described ( Zezula et al, 2019 ). Briefly, HPLC system (Shimadzu, Kyoto, Japan) fitted with a Hypersil® C-18 column (1.5 cm × 4.6 mm, 5 μm) was employed.…”