2023
DOI: 10.11002/kjfp.2023.30.3.383
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Development and validation of ultra-fast quantitative real-time PCR method to differentiate between Oncorhynchus keta and Oncorhynchus mykiss

Abstract: The ultra-fast quantitative real-time polymerase chain reaction (qPCR) assay was developed and validated to differentiate the morphologically similar ones, Oncorhynchus keta and Oncorhynchus mykiss. Species-specific primers were designed for the COI genes of mtDNA. The species-specific primers designed for O. keta and O. mykiss were selectively amplified by O. keta and O. mykiss D… Show more

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