Development and validation of UPLC method for quality control of Curcuma longa Linn.: Fast simultaneous quantitation of three curcuminoids

Jin, Cheng; Kong, Weijun; Luo, Yun; Wang, Jiabo; Wang, Haitao; Li, Qingmiao; Xiao, Xiaohe

doi:10.1016/j.jpba.2010.03.021

Cited by 70 publications

(33 citation statements)

References 30 publications

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“…Chromatographic methods are extensively used for quantitative analysis of curcuminoids and also for fingerprint analysis of Curcuma species. A number of qualitative and quantitative analysis of curcuminoids using thin layer chromatography (Sotanaphun et al 2009), HPLC with UV or fluorescence detector (Jadhav et al 2007;Wichitnithad et al 2009;Cheng et al 2010;Li et al 2011), liquid chromatography-mass spectrometry (LC-MS) (Verma et al 2013), micellar electrokinetic chromatography (Watanabe et al 2000), and capillary electrophoresis (Lechtenberg et al 2004;Anubala et al 2014) have been reported in various Curcuma samples. In the chromatographic fingerprint analysis of Curcuma species, the reported analytical instruments used are HPLC (Li et al 2011;Lee et al 2014) and GC (Lee et al 2014;Hu et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Curcuminoid’s Content and Fingerprint Analysis for Authentication and Discrimination of Curcuma xanthorrhiza from Curcuma longa by High-Performance Liquid Chromatography-Diode Array Detector

et al. 2015

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An accurate and reliable method for authentication and discrimination of Curcuma xanthorrhiza (CX) from Curcuma longa (CL) by determining the curcuminoid's content and analyzing the HPLC fingerprint combined with discriminant analysis (DA) was developed. By using the proposed method, it was found that CL had higher amount of all curcuminoid compounds compared to CX. Therefore, these two closely related species could be authenticated and discriminated by the amount of curcuminoids present in the samples. Authentication and discrimination of the two species were also achieved by comparing their HPLC fingerprint chromatograms using their typical marker peaks. To be more convincing, an aid from DA was also used. Combination of HPLC fingerprint analysis and DA gave excellent result that the two species were separated clearly, including CX samples adulterated with CL. The developed method was successfully used for quality control of the two plants.

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Section: Introductionmentioning

confidence: 99%

Curcuminoid’s Content and Fingerprint Analysis for Authentication and Discrimination of Curcuma xanthorrhiza from Curcuma longa by High-Performance Liquid Chromatography-Diode Array Detector

et al. 2015

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“…There have been numerous HPLC studies published detailing the separation of the three principle curcuminoids found in turmeric [12][13][14][15]. The application of these methods is limited due to poor separation resulting in overlapping peaks and peak tailing.…”

Section: Linearitymentioning

confidence: 99%

Purity Determination of Three Curcuminoids Found in Ten Commercially Available Turmeric Dietary Supplements Using a Reverse Phase HPLC Method

Shervington¹,

Ingham²,

Shervington³

2016

Nat Prod Chem Res

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The main active component present in turmeric is curcumin and is subsequently responsible for some of its therapeutic effects. Turmeric, however, contains two other active molecules, namely demethoxycurcumin (DMC) and bisdemethoxycurcumin (BDMC). Collectively, these three molecules are known as curcuminoids. Commercially available dietary supplements vary in these components. In this study the curcuminoids were analyzed using a reverse phase ion-pairing HPLC method. UV detection was used to detect the three curcuminoids at a single wavelength of 430 nm. The validation of the method was carried to ICH guidelines. Good repeatability of the method was achieved at concentrations of curcumin, DMC and BDMC equivalent to 136, 148 and 162 µM, respectively, with R.S.D. values of 2.25, 1.93 and 1.64%, respectively. Similarly, good reproducibility and linearity of the method was obtained for curcumin, DMC and BDMC. On completion of the validation, 10 commercially available turmeric dietary supplements were analyzed for their curcuminoid content. Results showed two of the turmeric dietary supplements to contain near pure curcumin. The other supplements tested contained significant amounts of DMC and BDMC. Similarly, there was a significant increase in curcuminoid content between supplements that contained turmeric extract and those that contained raw turmeric. Interestingly, supplements containing turmeric extract contained a significantly higher proportion of curcumin compared to DMC and BDMC, reflected in the ratio of curcumin: DMC: BDMC.

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“…Curcumin (phytochemical polyphenol) as main pigment of ginger herbal extracts (Zingiber officinale) and turmeric (Curcuma longa) known as functional food source in the nutritive industry [5][6][7]. Curcumin (principal curcuminoid of spice turmeric) with two other compounds like desmethoxycurcumin and bis-desmethoxycurcumin) [8][9][10] exhibit a keto-enol tautomerism [11,12] and therefore have diverce reactivity. Its daily application as condiment and spice has chemopreventive and chemotherapeutic which candidate it for treatment of ailments such as lung cancer, skin and liver diseases [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

A highly selective nanocomposite based on MIP for curcumin trace levels quantification in food samples and human plasma following optimization by central composite design

Bahrani

Mansoorkhani

Ostovan

2017

Journal of Chromatography B

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Development and validation of UPLC method for quality control of Curcuma longa Linn.: Fast simultaneous quantitation of three curcuminoids

Cited by 70 publications

References 30 publications

Curcuminoid’s Content and Fingerprint Analysis for Authentication and Discrimination of Curcuma xanthorrhiza from Curcuma longa by High-Performance Liquid Chromatography-Diode Array Detector

Curcuminoid’s Content and Fingerprint Analysis for Authentication and Discrimination of Curcuma xanthorrhiza from Curcuma longa by High-Performance Liquid Chromatography-Diode Array Detector

Purity Determination of Three Curcuminoids Found in Ten Commercially Available Turmeric Dietary Supplements Using a Reverse Phase HPLC Method

A highly selective nanocomposite based on MIP for curcumin trace levels quantification in food samples and human plasma following optimization by central composite design

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