Development and Validation of UV Spectrophotometric Method for the Determination of Cefuroxime Axetil in Bulk and Pharmaceutical Formulation

Abstract

The present study was undertaken to develop and validate a simple, sensitive, accurate, precise and reproducible UV spectrophotometric method for cefuroxime axetil using methanol as solvent. In this method the simple UV spectrum of cefuroxime axetil in methanol was obtained which exhibits absorption maxima (λ max ) at 278 nm. The quantitative determination of the drug was carried out at 278 nm and Beer's law was obeyed in the range of (0.80-3.60) µg/ml. The proposed method was applied to pharmaceutical formulation and percent amount of drug estimated (95.6% and 96%) was found in good agreement with the label claim. The developed method was successfully validated with respect to linearity, specificity, accuracy and precision. The method was shown linear in the mentioned concentrations having line equation y = 0.05x + 0.048 with correlation coefficient of 0.995. The recovery values for cefuroxime axetil ranged from 99.85-100.05. The relative standard deviation of six replicates of assay was less than 2%. The percent relative standard deviations of inter-day precision ranged between 1.45-1.92% and intra-day precision of cefuroxime axetil was 0.96-1.51%. Hence, proposed method was precise, accurate and cost effective.

Performance Characteristics of UV and Visible Spectrophotometry Methods for Quantitative Determination of Norfloxacin in Tablets

“…The methods were validated according to the International Conference on Harmonization (ICH) guidelines for validations of analytical methods [25,26].…”

Formulation and Evaluation of Cefuroxime Axetil Granules for Oral Suspension

“…Absorbance of each solution was measured at 278 nm. The standard curve was obtained by plotting concentration (μg/mL) values in X-axis and the absorbance values in Y-axis [14].…”

Determination of Cefuroxime Axetil in Tablet by HPLC, UV and First-Order Derivative Spectroscopy Methods & Plasma by UV Spectroscopy Method

“…In the literature reviews of cefuroxime axetil, which has a wide area of use as an antibiotic, scientific studies have been reached in the determination of plasma and pharmaceutical preparations (7,8). These methods in pharmaceutical preparations: spectrofluorometric method (9), HPLC (10-13), thin-layer chromatography and densitometry (14), capillary zone electrophoresis (15) and spectroscopy methods (16)(17)(18)(19).…”

“…In the studies based on spectroscopic analyses, cefuroxime axetil was derivatized by reacting with different reagents (1-nitroso-2 naphthol and sodium hydroxide; bromothymol blue) and analyzed by spectrophotometric method (16,17). Pritam et al analyzed cefuroxime axetil in eye drops in HCl medium with spectrophotometer (18). Game et al, (19) on the other hand, developed a UV spectrophotometric method in 0.1 M HCl medium and a derivative spectrophotometric method in 0.1 M NaOH medium for the determination of cefuroxime axetil in tablets.…”