Development and Validation of UV-Visible Spectrophotometric Method for the Determination of 5-Hydroxymethyl Furfural Content in Canned Malt Drinks and Fruit Juices in Ghana

Abstract

A simple, rapid, accurate, and less expensive spectrophotometric method has been developed for the quantitation of 5-hydroxymethyl furfural (5-HMF) levels in canned malt drinks and fruit juice drinks sampled in the Kumasi Metropolis, Ghana. The quantitation is based on the selective maximum absorption of ultraviolet radiation by 5-HMF at the wavelength (λmax) of 284 nm using acetonitrile : water (50 : 50 v/v) as the solvent system. The method was established to be specific, precise, and accurate over a concentration range of 0.001 mg/ml–0.02 mg/ml. 5-HMF levels in fruit juice samples (A1–A10) were between 0.132 mg/ml and 0.438 mg/ml, and these levels were shown to be comparable (t = 2.200;p=0.0553) to the contents in the canned malt samples (M1–M10) which were between 0.3140 mg/ml and 0.7170 mg/ml. The study failed to show any dependence of 5-HMF levels on the composition of the product as well as the manufacturing process adopted. The length of storage did also not significantly affect the 5-HMF levels in the products.

Kinetic Evaluation of the Production of Mead from a Non-Saccharomyces Strain

“…The density was evaluated by the pycnometer method [ 44 ], and the total acidity, the total sulfur dioxide, and the free sulfur dioxide were evaluated by volumetric titration [ 45 , 46 ]. The content of 5-hydroxymethylfurfural (HMF) (Sigma Aldrich, Toluca, Mexico State, Mexico) was evaluated by the dilution of the corresponding sample, making an HMF curve from an acetonitrile: water (50:50 v / v ) solution with a concentration of 0 to 10 μg/mL, read on a spectrophotometer at 284 nm [ 47 ]. The aldehydes, esters, methanol, and higher alcohols were quantified by gas chromatography (Clarus 480, Perkin Elmer, Whatman, MA, USA) [ 48 ].…”

Detection of 2-Furaldehyde in Milk by MIP-Based POF Chips Combined with an SPR-POF Sensor

“…Consequently, developing sensitive and accurate methods to detect furfurals in milk is essential. Classical analyses are based on chromatographic or spectrophotometric techniques [ 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 ]. Chromatographic methods are undoubtedly the most common, but some of them have scarce specificity; moreover, the instruments are bulky and require toxic reagents and solvents.…”