Development and validation of UV spectrophotometric method for orbifloxacin assay and dissolution studies

Cazedey, Edith Cristina Laignier; Salgado, Hérida Regina Nunes

doi:10.1590/s1984-82502014000300003

Cited by 11 publications

(4 citation statements)

References 45 publications

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“…[62][63][64][65] However, it is a non-selective method and is not suitable for the detection of degradation products and impurities. [66] Spectrophotometric methods are also found in literature for other fluoroquinolones, such as ciprofloxacin, [67] norfloxacin, [68,69] orbifloxacin, [70] lomefloxacin, [71] gatifloxacin, [72] and sparfloxacin. [73,74] Costa et al (2014) developed and validated a microbiological agar diffusion method for the potency analysis of besifloxacin in ophthalmic suspension.…”

Section: Uv (289 Nm)mentioning

confidence: 99%

Besifloxacin: A Critical Review of Its Characteristics, Properties, and Analytical Methods

Tótoli

Salgado

2018

Critical Reviews in Analytical Chemistry

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Bacterial conjunctivitis has high impact on the health of the population, since it represents more than a third of ocular pathologies reported by health services worldwide. There is a high incidence of bacterial resistance to the antimicrobials most commonly used for the treatment of conjunctivitis. In this context, besifloxacin stands out, since it is a fluoroquinolone developed exclusively for topical ophthalmic use, presenting a low risk of developing resistance due to its reduced systemic exposure. Bausch & Lomb markets it as ophthalmic suspension, under the trade name Besivance™. Literature review on besifloxacin is presented, covering its pharmaceutical and clinical characteristics, and the analytical methods used to measure the drug in pharmaceutical products and biological samples. High performance liquid chromatography is the most used method for this purpose. A discussion on Green Chemistry is also presented, focusing the importance of the development of green analytical methods for the analysis of drugs.

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Section: Uv (289 Nm)mentioning

confidence: 99%

Besifloxacin: A Critical Review of Its Characteristics, Properties, and Analytical Methods

Tótoli

Salgado

2018

Critical Reviews in Analytical Chemistry

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“…Detection and quantification limits (DL and QL) were calculated from Equations (2) and (3), achieving the following values: DL = 0.86 ± 0.91 μg·mL −1 (quercetin) and 9.02 ± 1.12 μg·mL −1 (rutin); QL = 2.88 ± 0.92 μg·mL −1 (quercetin) and 30.09 ± 1.01 μg·mL −1 (rutin). To determined selectivity of the method, the extracts’ chromatograms were compared to standard chromatograms [30,41]. The extracts and mixed sample did not exhibit impurities or other compounds capable to interfere in identification of quercetin and rutin peaks.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of In Vitro Solar Protection Factor (SPF), Antioxidant Activity, and Cell Viability of Mixed Vegetable Extracts from Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L.

Cefali

Ataide

Fernandes

et al. 2019

Plants

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The aim of this study was to validate a HPLC method for the assay of flavonoids in extracts obtained from natural sources, i.e., from Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L. The potential sun protecting effect, antioxidant activity, and cell viability of the extracts were also determined. Individual extracts (obtained from each individual species) and a mixed extract (containing the four extracts) were analyzed by the validated HPLC method for the identification of flavonoids and quantification of rutin and quercetin. An in vitro cell viability study was carried out using the neutral red method. The in vitro sun protection factor was determined by spectral transmittance and in vitro antioxidant efficacy was evaluated against DPPH, ABTS, and AAPH radicals. The HPLC method used for the identification and quantification of flavonoids in extracts exhibited linearity, precision, accuracy, and robustness. Detection and quantification limits were, respectively, 2.881 ± 0.9 μg·mL−1 and 0.864 ± 0.9 μg·mL−1 for quercetin, and 30.09 ± 1 μg·mL−1 and 9.027 ± 1.1 μg·mL−1 for rutin. All extracts did not affect cell viability at the evaluated concentration range and exhibited a sun protection effect and antioxidant activity. Among the evaluated extracts, Ginkgo biloba L. and the mixed extract depicted the most expressive antioxidant activity. The mixed extract exhibited sunscreen protection against ultraviolet A (UVA) and ultraviolet B (UVB) and a critical wavelength of 372.7 ± 0.1. Our results translate the enhanced flavonoids’ composition of the mixed extract, which may be a potential alternative over sunscreens and antioxidants in pharmaceutic/cosmetic formulations.

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“…Robustness was tested by varying detection wavelength (±2 nm) of optimized conditions from the standard detection wavelength (305.6 nm) 16 .…”

Section: Robustnessmentioning

confidence: 99%

Untitled

2018

IJPSR

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ABSTRACT:The objective of the present study is to develop and validate a novel stability indicating UV spectrophotometric method for estimation of Benzydamine Hydrochloride (BNZ) in pharmaceutical dosage form as not a single UV spectrophotometric method has been reported for the estimation of Benzydamine Hydrochloride, which makes the present work novel. In the present research work, UV spectrophotometric determination was carried out at an absorption maximum of 305.6 nm in pH 6.8 phosphate buffer. The purposed method was validated statistically for linearity, accuracy, precision, repeatability, ruggedness, sensitivity as per International Conference on Harmonization guidelines. The drug substance was exposed to acid hydrolysis, alkaline hydrolysis, oxidative degradation, photolytic degradation, and thermal degradation and the stressed samples were analyzed by the proposed method to demonstrate the specificity of the method. The application of developed method was established by performing the assay of pharmaceutical formulation. The proposed method obeyed beer lambert's law in the concentration range of 5 -50 µg ml -1 with correlation coefficient (r 2 ) of 0.999. Percentage relative standard deviation associated with all the validation parameters were ≤ 2%. There were no significant changes in absorbance after performing the forced degradation studies and the % age drug recovery was found to be 98.53% and 98.53% for film and gel respectively. The proposed method was found to be novel, simple, rapid, precise, selective, reproducible economical and stability indicating can be successfully applied to the determination of Benzydamine Hydrochloride in bulk and in pharmaceutical dosage form.

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Development and validation of UV spectrophotometric method for orbifloxacin assay and dissolution studies

Cited by 11 publications

References 45 publications

Besifloxacin: A Critical Review of Its Characteristics, Properties, and Analytical Methods

Besifloxacin: A Critical Review of Its Characteristics, Properties, and Analytical Methods

Evaluation of In Vitro Solar Protection Factor (SPF), Antioxidant Activity, and Cell Viability of Mixed Vegetable Extracts from Dirmophandra mollis Benth, Ginkgo biloba L., Ruta graveolens L., and Vitis vinífera L.

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