Development, characterization and cross-species amplification of mungbean (Vigna radiata) genic microsatellite markers

Abstract

In this paper, we report the development and characterization of genic microsatellite markers for mungbean by mining sequence database, and transferability of the markers to Asian Vigna species. A total of 157 markers were designed upon searching for SSR in 830 transcript sequences. Thirty-three loci revealed single copy polymorphism in a panel of 17 mungbean accessions with number of alleles ranging from 2 to 8, and observed heterozygosity from 0 to 0.3125. Seven loci deviated from Hardy-Weinberg Equilibrium. Two pairwise combinations of allele frequencies implied linkage disequilibrium. Cross-species amplification in 19 taxa of Asian Vigna using 85 primers showed that amplification rates varied between 80% (V. aconitifolia) to 95.3% (V. reflexo-pilosa). These mungbean genic microsatellite markers will be useful to study genetic resource and conservation of Asian Vigna species.

Development of new gene-specific markers associated with salt tolerance for mungbean (Vigna radiata L. Wilczek)

“…All microsatellite markers used in this study showed considerable amplif ication and highly signif icant polymorphism (86.84-100%) within the analyzed genotypes from different Vigna species. The obtained polymorphism is significantly higher as compared to that reported earlier (Chaitieng, 2006;Somta et al, 2009;Sudha et al, 2012). The SSR markers were developed from the candidate gene Glycine max, which is involved in the SOS pathway.…”

QTLs CONTROLLING SEED WEIGHT AND DAYS TO FLOWERING IN MUNGBEAN [Vigna radiata (L.) Wilczek], THEIR CONSERVATION IN AZUKI BEAN [V. angularis (Ohwi) Ohwi & Ohashi] AND RICE BEAN [V. umbellata (Thunb.) Ohwi & Ohashi]

“…KPS1 and V4718 were screened for DNA polymorphism using 753 primer pairs of simple sequence repeat (SSR) markers [433 from mungbean (Gwag et al, 2006;Tangphatsornruang et al, 2009;Somta et al, 2008;Seehalak et al, 2009;Somta et al, 2009) et al, 2001), and 86 from common bean (Phaseolus vulgaris L.) (Gaitán-Solís et al, 2002;Blair et al, 2003;Guerra-Sanz, 2004;Buso et al, 2006)]. Polymorphic markers were then used to analyze the F2 population.…”

Evaluation of Mungbean Mutant Lines to Drought Stress and Their Genetic Relationships Using SSR Markers

“…The concentrations of the DNA samples were determined with a spectrophotometer, and the DNA samples were diluted to 50 ng/μl for analysis using SSR markers. A total 23 SSR primers derived from mungbean [20] and soybean sequences [21] were used in this study. PCR analysis was conducted in a volume of 15 μl which contained 20 ng/µl of genomic DNA, 0.25 μM of each primer (forward and reverse), 0.125 mM of each dNTP, 0.15 units of TaqDNA polymerase, and 1× reaction buffer.…”