Development, characterization and pre-clinical trials of an innovative wound healing dressing based on propolis (EPP-AF®)-containing self-microemulsifying formulation incorporated in biocellulose membranes

Marquele-Oliveira, Franciane; Barud, Hernane da Silva; Torres, Elina Cassia; Machado, Rachel Temperani Amaral; Caetano, Guilherme Ferreira; Leite, Marcel Nani; Frade, Marco Andrey Cipriani; Ribeiro, Sidney J. L.; Berretta, Andresa Aparecida

doi:10.1016/j.ijbiomac.2019.05.135

Cited by 40 publications

(38 citation statements)

References 48 publications

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“…This network architecture confers to BC tremendous possibilities to be used in various fields but especially in the medical field as a skin wound and burn cure, artificial skin, artificial blood vessels, dental implants, scaffolds for tissue engineering, and drug delivery systems, with the porous structure ensuring an efficient cell attachment and high capacity of exudate adsorption [ 8 ]. It is characterized by a good permeability and resistance to degradation, good mechanical properties, high purity and high water absorption capacity, remarkable biocompatibility, and high porosity [ 1 , 9 , 10 , 11 , 12 , 13 ]. Not only BC but also BC composites are considered promising wound dressing materials [ 14 ].…”

Section: Introductionmentioning

confidence: 99%

“…The particular nano-fibrillar structure of BC is a suitable macromolecular support for the adsorption of drugs and therefore for the development of specific controlled release systems, including wound dressing and transdermal drug delivery systems [ 15 ]. The BC membrane’s ability to facilitate active substances’ release for percutaneous administration has been demonstrated [ 12 ]. It is also a neutral electrostatic material, which allows both negative and positive charges of bioactive compounds.…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial Activity of Bacterial Cellulose Loaded with Bacitracin and Amoxicillin: In Vitro Studies

et al. 2020

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The use of bacterial cellulose (BC) in skin wound treatment is very attractive due to its unique characteristics. These dressings’ wet environment is an important feature that ensures efficient healing. In order to enhance the antimicrobial performances, bacterial-cellulose dressings were loaded with amoxicillin and bacitracin as antibacterial agents. Infrared characterization and thermal analysis confirmed bacterial-cellulose binding to the drug. Hydration capacity showed good hydrophilicity, an efficient dressing’s property. The results confirmed the drugs’ presence in the bacterial-cellulose dressing’s structure as well as the antimicrobial efficiency against Staphylococcus aureus and Escherichia coli. The antimicrobial assessments were evaluated by contacting these dressings with the above-mentioned bacterial strains and evaluating the growth inhibition of these microorganisms.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Bacterial Cellulose Loaded with Bacitracin and Amoxicillin: In Vitro Studies

et al. 2020

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“…The antimicrobial activity and the wound healing properties of novel BC containing Brazilian propolis was also demonstrated by Wei et al [171] , Marquele-Oliveira et al [172] and Picolotto et al [173] . The first two authors proved the antimicrobial efficacy of in vitro BC/propolis membranes against Gram-negative and Gram-positive bacteria; their in vitro studies suggested that the biomaterial may promote fast re-epithelization and tissue organization, setting up a potential therapy for infected wounds.…”

Section: Brazilmentioning

confidence: 62%

Commercial and potential applications of bacterial cellulose in Brazil: ten years review

et al. 2020

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“…Chitosan-based coacervates prepared for propolis encapsulation have been reported to be non-cytotoxic [63]. Another study proved that propolis and essential oils incorporated in biocellulose membranes exhibited antibacterial and proinflammatory activity, and improved wound healing in rats [64]. Other materials used for propolis incorporation are corn starch dressings [65] and polyurethane-hyaluronic acid nanofibrous dressings [66].…”

Section: Discussionmentioning

confidence: 99%

Biofilm Formation Reducing Properties of Manuka Honey and Propolis in Proteus mirabilis Rods Isolated from Chronic Wounds

et al. 2020

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Chronic wound infections are difficult to manage because of the biofilm formation in the wound environment. New measures for eliminating infections are necessary to increase the chance of wound healing. Apitherapy may be the new solution. The aim of this study was to assess the prevalence of wound infection factors and to examine the impact of Manuka honey and ethanol extract of propolis on biofilm formation of Proteus mirabilis isolated from chronic wound infections. According to the findings, the most frequent factors of infection are Staphylococcus aureus (46.1%), Pseudomonas aeruginosa (35.0%), and Proteus mirabilis (10.6%). Minimal inhibitory concentration and minimal bactericidal concentration values were assigned using the microbroth dilution test according to the Clinical and Laboratory Standards Institute. Biofilm of Proteus mirabilis isolates was formed in 96-well polystyrene plates and treated with Manuka honey (concentrations from 1.88% to 30.0%) and ethanol extract of propolis (1.0% to 40.0%). After 24 h, the biofilm viability was expressed by formazan absorbance (λ = 470 nm). Manuka honey reduced the biofilm viability in all, and ethanol extract of propolis in most, of the concentrations tested. Ethanol extract of propolis at the concentrations of 20.0% and 40.0%, reduced biofilm viability stronger than ethanol itself. With these results comes the conclusion that these substances can reduce biofilm formation.

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Development, characterization and pre-clinical trials of an innovative wound healing dressing based on propolis (EPP-AF®)-containing self-microemulsifying formulation incorporated in biocellulose membranes

Cited by 40 publications

References 48 publications

Antibacterial Activity of Bacterial Cellulose Loaded with Bacitracin and Amoxicillin: In Vitro Studies

Antibacterial Activity of Bacterial Cellulose Loaded with Bacitracin and Amoxicillin: In Vitro Studies

Commercial and potential applications of bacterial cellulose in Brazil: ten years review

Biofilm Formation Reducing Properties of Manuka Honey and Propolis in Proteus mirabilis Rods Isolated from Chronic Wounds

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