Development, characterization, and transferability of microsatellite markers for Kirengeshoma palmata (Hydrangeaceae)

Abstract

These microsatellite markers provide a useful tool to investigate the mating system, gene flow, parentage, and population dynamics of Kirengeshoma.

Understanding population structure and historical demography in a conservation context: Population genetics of the endangeredKirengeshoma palmata(Hydrangeaceae)

“…We used a total of 12 microsatellite markers (nSSRs: Kp05, Kp15, Kp27, Kp28, Kp36, Kp42, Kp48, Kp49, Kp51, Kp65, Kp69, Kp74) developed specifically for K. palmata by Sun et al (2010). Primers were labeled with a fluorescent dye, either HEX or 6‐FAM (Applied Biosystems, Foster City, California, USA), and PCR amplifications were performed on a GeneAmp 9700 DNA Thermal Cycler (Perkin‐Elmer, Waltham, Massachusetts, USA) using protocols described by Sun et al (2010). PCR products were separated on a MegaBACE 1000 (GE Healthcare Biosciences, Pittsburgh, PA, USA).…”

Development of 12 microsatellite markers for Platycrater arguta (Hydrangeaceae) endemic to East Asia

“…Both the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol (Zane et al, 2002) and the compound microsatellite marker technique based on a dual‐suppression‐PCR method (Lian et al, 2006) were used to develop microsatellite markers for P. arguta . For the FIASCO technique, a microsatellite dinucleotide‐enriched genomic library was constructed following the protocol of Zane et al (2002) with some modifications (Sun et al, 2010). One individual of P. arguta var.…”