Development, evaluation and application of an isocratic high-performance liquid chromatography (HPLC) assay for the simultaneous determination of aciclovir and its metabolite 9-carboxymethoxymethylguanine in human serum and cerebrospinal fluid

Darville, J. M.; Lovering, Andrew; MacGowan, Alasdair

doi:10.1016/j.ijantimicag.2007.02.005

Cited by 7 publications

(8 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…PBMCs have been used as sample materials for analysis of amprenavir, efavirenz, indinavir, lopinavir, nelfinavir (some also its metabolite M8), nevirapine, and ritonavir. Pelerin et al [ Other antiviral drugs HPLC in combination with UV detection is often used for TDM of other antiviral drugs, mostly in plasma [45][46][47][48][49][50][51][52][53][54][55] but also in cerebrospinal fluid [56] and-for the same reasons as antiretroviral drugs-intracellularly [57].…”

Section: Antiretroviral Drugsmentioning

confidence: 99%

Therapeutic drug monitoring by LC–MS–MS with special focus on anti-infective drugs

Müller

Rentsch

2010

Anal Bioanal Chem

View full text Add to dashboard Cite

Liquid chromatography coupled to mass spectrometry nowadays plays an important role in the field of therapeutic drug monitoring (TDM), especially of new compounds for which no immunoassays are available. This paper reviews LC-MS(-MS) methods published recently for anti-infective drugs: antiretroviral drugs, other antiviral drugs, antibacterial drugs, antihelmintic drugs, antimalarial drugs, and other antiprotozoal drugs. An overview of the different methods is given, with special focus on selection of the internal standard and validation procedures.

show abstract

Section: Antiretroviral Drugsmentioning

confidence: 99%

Therapeutic drug monitoring by LC–MS–MS with special focus on anti-infective drugs

Müller

Rentsch

2010

Anal Bioanal Chem

View full text Add to dashboard Cite

show abstract

“…Deneme -yanılma adı verilen bu yaklaşım, herhangi bir sistematik temele dayanmadığı için birçok deneysel kombinasyonu içermektedir ve özellikle polar bileşiklerin tayini olmak üzere, optimum ayırma koşulunu belirlemek oldukça zorlaşmaktadır [6][7][8][9][10]. Literatürde valasiklovir tayininde de deneme-yanılma yaklaşımının tercih edildiği görülmektedir [11][12][13][14][15][16][17][18][19][20][21]. Valasiklovir için literatürde bulunan çalışmaların çoğu RPLC yöntemle ve izokratik modda gerçekleştirilmesiyle beraber, bileşiğin tablet formülasyonunda [11][12][13][14][15][16][17] ve biyolojik sıvılardaki tayiniyle alakalıdır [18][19][20][21].…”

Section: Introductionunclassified

Development and Validation of the RPLC Method for the Determination of Valaciclovir in Human Urine

DALDAL

2023

Süleyman Demirel Üniversitesi Sağlık Bilimleri Dergisi

View full text Add to dashboard Cite

Sunulan çalışmada, herpes ve megalovirüslerin sebep olduğu enfeksiyonların tedavisinde tercihli olarak kullanılan ilaçlardan valasiklovirin katkılandırılmış idrarda ters faz sıvı kromatografik yöntemle tayini için yöntem geliştirilmiştir. Yöntem geliştirmede, valasiklovirin sistematik olarak pH-kapasite faktörü ilişkisini değerlendirilip, mobil faz optimizasyonu gerçekleştirilmiştir. İnceleme sonunda, optimum ayırma koşulu %4 asetonitril-su (h/h), pH 5,0 ve 37oC kolon sıcaklığı olarak belirlenmiştir. Sonrasında, belirlenen optimum koşulun Uluslararası Harmonizasyon Topluluğu (ICH) yönergelerine göre validasyonu gerçekleştirilmiştir. Geliştirilen yöntem 2-12 μg/mL derişim aralığında muhteşem doğrusallık göstermiştir ve idrar ortamında gerçekleştirilen geri kazanma sonuçları (%) valasiklovirin iki farklı derişimi için 100,147±0,800 ve 100,208±0,604 olarak bulunmuştur. Ayrıca, geliştirilen yönteme sağlamlık testleri de uygulanmıştır ve elde edilen sonuçlar t-testiyle değerlendirilmiştir. Elde edilen validasyon ve sağlamlık testleri sonucunda, geliştirilen yöntemin hassas, tekrar edilebilir, doğru, kesin ve sağlam olduğu bulunmuştur. Bundan dolayı, geliştirilen yöntemin rutin analizler için uygun olduğu belirlenmiştir.

show abstract

“…Previously published analytical methods for determination of ACV and its metabolite CMMG have used HPLC in reversed‐phase mode for the separation followed by UV, UV‐diode array, fluorescence detection, or MS (Dao et al, 2008 ; Darville et al, 2007 ; Svensson et al, 1997 ; Urinovska et al, 2021 ; Weller et al, 2009 ). In these publications, the most common reversed phases used for chromatographic separation have been various C8 and C18 phases with, for example, HSS T3 tri‐functionally bonded C18 ligands that promote polar compound retention and are compatible with aqueous mobile phases.…”

Section: Introductionmentioning

confidence: 99%

“…Combining the peak capacity of the UHPLC column with the selectivity of the mass spectrometer generally results in increased sensitivity and reduced analysis time, particularly for multicompound methods. Several methods exist for the determination of ACV, GCV, and PCV in human plasma or serum, with or without simultaneous determination of their corresponding prodrugs (Heinig et al, 2011 ; Kasiari et al, 2008 ; Schimek et al, 2018 ; Shi et al, 2018 ; Xu et al, 2007 ; Yadav et al, 2009 ) but only a few methods include the measurement of CMMG (Darville et al, 2007 ; Svensson et al, 1997 ; Urinovska et al, 2021 ; Yang et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

Rapid determination of acyclovir, its main metabolite 9‐carboxymethoxymethylguanine, ganciclovir, and penciclovir in human serum using LC–MS/MS

Ärlemalm

Helldén

Karlsson

et al. 2022

Biomedical Chromatography

View full text Add to dashboard Cite

A novel MS-based analytical method for simultaneous analysis of the antiviral drugs acyclovir, its metabolite 9-carboxymethoxymethylguanine, ganciclovir, and penciclovir in human serum is described. These antiviral drugs are active against herpes virus infections. Acyclovir and penciclovir are regarded as safe and effective medicines with mild side effects such as headache and gastrointestinal discomfort, and ganciclovir is regarded as more toxic and is known to cause, for example, bone marrow suppression. Acyclovir's main metabolite 9-carboxymethoxymethylguanine is a presumptive neurotoxin and should be monitored in patients with impaired renal function or in cases with neurotoxic symptoms. A sample was prepared using protein precipitation with 1% formic acid in methanol containing isotopically labeled internal standard. Chromatographic separation on a biphenyl column and mass spectrometric detection were performed in multiple reaction monitoring (MRM) mode on a Xevo TQ-S micro with ESI in positive ion mode, within 3 min. Inter-day assay accuracies for the quality controls varied between 95 and 104% and intra-day assay between 93 and 105%. Inter-day and intra-day assay imprecision for the quality controls ranged between 1.4 and 4.2% and 1.7 and 6.5% respectively. The lower limit of quantification for all four substances was 0.156 μmol/L. It is an accurate and reproducible method for therapeutic drug monitoring.

show abstract

Development, evaluation and application of an isocratic high-performance liquid chromatography (HPLC) assay for the simultaneous determination of aciclovir and its metabolite 9-carboxymethoxymethylguanine in human serum and cerebrospinal fluid

Cited by 7 publications

References 6 publications

Therapeutic drug monitoring by LC–MS–MS with special focus on anti-infective drugs

Therapeutic drug monitoring by LC–MS–MS with special focus on anti-infective drugs

Development and Validation of the RPLC Method for the Determination of Valaciclovir in Human Urine

Rapid determination of acyclovir, its main metabolite 9‐carboxymethoxymethylguanine, ganciclovir, and penciclovir in human serum using LC–MS/MS

Contact Info

Product

Resources

About