Development, genotype and phenotype of a new colony of the Sabra hypertension prone (SBH/y) and resistant (SBN/y) rat model of salt sensitivity and resistance

Yagil, Chana; Katni, Gurion; Rubattu, Speranza; Stolpe, Christoph; Kreutz, Reinhold; Lindpaintner, Klaus; Ganten, Detlev; Ben-lshay, Drori; Yagil, Yoram

doi:10.1097/00004872-199610000-00004

Cited by 50 publications

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“…In the parental strains (SBH/y and SBN/y), there are no sex differences in basal BP or BP after salt loading within each strain. 3 In F 2 generations of crosses 1 and 2 in the current study, average BP after salt loading was lower in females than in males. Of note is that BP before salt loading did not differ between the sexes (data not shown).…”

Section: Discussionmentioning

confidence: 44%

“…SBH/y and SBN/y (Barzilai Medical Center colony, Ashkelon, Israel) 3 were crossbred. The initial cross (cross 1a) was between 7 female SBH/y and 2 male SBN/y.…”

Section: Animalsmentioning

confidence: 99%

“…3 At least 3 replicate BP measurements were made by the same operator on 3 consecutive days (ie, at least 9 measurements over 3 days), both at baseline and after salt loading. The average of all measurements at each experimental time point was taken as representative of systolic BP.…”

Section: Phenotypingmentioning

confidence: 99%

“…3 Purity and quantity of extracted DNA were assessed spectrophotometrically (GeneQuant II, Pharmacia Biotech).…”

Section: Dna Extractionmentioning

confidence: 99%

“…3 In brief, genomic DNA (50 ng) was amplified by PCR. The forward primer was labeled with [ 32 P]ATP (Dupont NEN) using T4 polynucleotide kinase (Promega).…”

Section: Genotypingmentioning

confidence: 99%

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Role of Chromosome X in the Sabra Rat Model of Salt-Sensitive Hypertension

et al. 1999

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Abstract-We carried out a total genome screen in the Sabra rat model of hypertension to detect salt-susceptibility genes.We previously reported in male animals the presence of 2 major quantitative trait loci (QTLs) on chromosome 1 that together accounted for most of the difference in the blood pressure (BP) response to salt loading between Sabra hypertension-prone rats (SBH/y) and Sabra hypertension-resistant rats (SBN/y). In females, we reported on 2 major QTLs on chromosomes 1 and 17 that together accounted for only two thirds of the difference in the BP response between the strains. On the basis of phenotypic patterns of inheritance in reciprocal F 2 crosses, we proposed a role of the X chromosome. We therefore continued the search for the missing QTL in females that would account for the remaining difference in the BP response between the 2 strains using newly developed microsatellite markers and focusing on chromosome X. We screened an F 2 cross, consisting of 371 females and 336 males, using 19 polymorphic chromosome X microsatellite markers. We analyzed the averages of BP by genotype using ANOVA and the individual data using MAPMAKER/QTL. In female F 2 progeny, we identified a segment on chromosome X that spans over 33.4 cM and shows significant cosegregation (PϽ0.001) of 14 microsatellite markers (demarcated by DXRat4 and DXMgh10) with systolic BP after salt loading. This segment has 2 apparent peaks at DXRat4 and DXRat13, with a BP effect of 14 mm Hg for each. Multipoint linkage analysis with a free model detected 3 peaks (logarithm of the odds ratio [LOD] score Ͼ4.3) within the same chromosomal segment: One between DXMgh9 and DXMit4 (LOD 4.9; 6.1% of variance), a second between DXMgh12 and DXRat8 (LOD 5.2; 7.2% of variance), and a third between DXRat2 and DXRat4 (LOD 5.8; 7.5% of variance). On the basis of these findings and until congenic strains become available, our working assumption is that within chromosome X, 1 to 3 genetic loci contribute importantly to the BP response of female Sabra rats to salt. In male F 2 progeny, we detected no significant cosegregation of any region on chromosome X with the BP response to salt loading. We conclude that in the female rat, salt susceptibility is mediated by 3 to 5 gene loci on chromosomes 1, 17, and X, whereas in the male rat, the X chromosome does not affect the BP response to salt.

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Section: Discussionmentioning

confidence: 44%

“…SBH/y and SBN/y (Barzilai Medical Center colony, Ashkelon, Israel) 3 were crossbred. The initial cross (cross 1a) was between 7 female SBH/y and 2 male SBN/y.…”

Section: Animalsmentioning

confidence: 99%

Section: Phenotypingmentioning

confidence: 99%

“…3 Purity and quantity of extracted DNA were assessed spectrophotometrically (GeneQuant II, Pharmacia Biotech).…”

Section: Dna Extractionmentioning

confidence: 99%

“…3 In brief, genomic DNA (50 ng) was amplified by PCR. The forward primer was labeled with [ 32 P]ATP (Dupont NEN) using T4 polynucleotide kinase (Promega).…”

Section: Genotypingmentioning

confidence: 99%

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Role of Chromosome X in the Sabra Rat Model of Salt-Sensitive Hypertension

et al. 1999

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Rat Models of Cardiovascular Diseases

Bäder

2009

Methods in Molecular Biology

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In cardiovascular research, the rat has been the main model of choice for decades. Experimental procedures were developed to generate cardiovascular disease states in this species, such as systemic and pulmonary hypertension, cardiac hypertrophy and failure, myocardial infarction, and stroke. Furthermore, rats have been bred, which spontaneously develop such diseases. They became extremely valuable models to understand the genetics of these diseases, since powerful genomic tools are now available for the rat. One of these tools is transgenic technology, which has allowed the creation of even more disease models in the rat. This review summarizes the experimental, genetic, and transgenic rat models for cardiovascular diseases.

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Models of Hypertension and Blood Pressure Recording

Campos

Baltatu

2012

Methods in Pharmacology and Toxicology

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Development, genotype and phenotype of a new colony of the Sabra hypertension prone (SBH/y) and resistant (SBN/y) rat model of salt sensitivity and resistance

Cited by 50 publications

References 0 publications

Role of Chromosome X in the Sabra Rat Model of Salt-Sensitive Hypertension

Role of Chromosome X in the Sabra Rat Model of Salt-Sensitive Hypertension

Rat Models of Cardiovascular Diseases

Models of Hypertension and Blood Pressure Recording

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