We designed and developed a carbon nanotube (CNT)-based reflective digital cell irradiation system capable of irradiating cells. The chemical and physical properties of the CNT synthesized directly on the patterned substrate were confirmed, and the field emission characteristics with a maximum anode current of 10 mA were evaluated through the I-V curve. Also, electrostatic simulation was conducted to confirm the electric field distribution and the electron beam trajectory. According to the duty (27.3 mGy at anode on time 10 sec, duty 50 %), the anode voltage (25.2 mGy at anode on time 10 sec, duty 50 %, anode voltage 40 kV) and the distance between the window and the cell stage (anode on time 10 sec, duty 50%, anode voltage 40 kV, 19.4 mGy), the function of the system was verified by obtaining the dose emitted from the system. This study confirmed that it is suitable as the cell irradiation system for studying the radiobiological effects of low-dose radiation-irradiated cells.