Development of 15 nuclear microsatellite markers in <i>Deuterocohnia</i> (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing

Zenk, Fides; Firmer, Cynthia; Wöhrmann, Tina; Vicente-Silva, Luciana; Weising, Kurt; Huettel, Bruno; Paggi, Gecele Matos

doi:10.1002/aps3.1147

Cited by 3 publications

(3 citation statements)

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“…The successful cross-amplification observed in this study (86% amplification and 46% polymorphism) was also reported for other bromeliads (Barbará et al 2007b;Palma-Silva et al 2007, 2009Paggi et al 2008;Wörhmann and Weising 2011;Zanella et al 2012;Goetze et al 2013;Lavor et al 2014;Aoki-Gonçalves et al 2014;Neri et al 2015;Ferreira et al 2017;Chaves et al 2018;Godoy et al 2018;Zenk et al 2018). The success of heterologous nuclear microsatellite transfer is usually related to the phylogenetic distance between tested taxa (Barbará et al 2007a).…”

Section: Resultssupporting

confidence: 82%

“…In the case of Bromeliaceae species, wide adaptive radiation has caused low levels of genomic divergence. Therefore, various researchers have been successfully transferring markers among species within subfamilies and even among subfamilies (Barbará et al 2007b(Barbará et al , 2009Palma-Silva et al 2007;Paggi et al 2008;Wörhmann and Weising 2011;Wörhmann et al 2012;Zanella et al 2012;Goetze et al 2013;Ferreira et al 2017;Chaves et al 2018;Godoy et al 2018;Meireles and Manos 2018;Zenk et al 2018). Thus, the present study aimed to test the cross-amplification of nuclear microsatellite markers, originally designed for other bromeliad species, in Aechmea distichantha.…”

Section: Introductionmentioning

confidence: 97%

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Cross-amplification of nuclear microsatellite markers in Aechmea distichantha Lem. (Bromeliaceae)

2019

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Aechmea distichantha Lem. is a Bromeliaceae species with wide geographic distribution; it is found in Atlantic Forest, "Cerrado" and "Chaco" ecoregions from Tropical to Subtropical areas in Brazil, Bolivia, Paraguay and Argentina. The species has a great ornamental value and is currently threatened by its predatory exploration and by habitat loss. The lack of suitable molecular markers hampers research on its genetic diversity, which could contribute to the design of conservation plans and to demographic and phylogeographic studies of the species. Here, we tested the cross-amplification of 43 nuclear microsatellite markers, originally developed for other bromeliad species. We obtained high cross-amplification indices (37 SSR primers, 86%) and polymorphism (17 SSR primers, 46%). We then used ten SSR loci to genotype individuals from three populations of A. distichantha. The observed and expected heterozygosity per locus in the A. distichantha populations ranged from 0.182 to 0.735 and 0.297 to 0.830, respectively. These loci showed sufficient variability to be used in future the studies of genetic diversity, genetic structure and phylogeography of A. distichantha to understand its evolutionary history during its dispersal, colonization and adaptation to different ecoregions.

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Section: Resultssupporting

confidence: 82%

Section: Introductionmentioning

confidence: 97%

Cross-amplification of nuclear microsatellite markers in Aechmea distichantha Lem. (Bromeliaceae)

2019

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“…Accordingly, the level of transferability obtained for the species studied here can be considered above average, since 12 of the 24 pairs of primers tested (50%) yielded positive results. Similarly successful results in marker transfer have also been reported for other species of Bromeliaceae (Barbará et al 2007a;Paggi et al 2008;Palma-Silva et al 2009Wöhrmann and Weising 2011;Zanella et al 2012;Goetze et al 2013;Lavor et al 2014;Neri et al 2015;Ferreira et al 2017;Pereira et al 2017;Chaves et al 2018;Godoy et al 2018Godoy et al , 2019Zenk et al 2018). High transferability between species of the same Bromeliaceae subfamily is probably due to the family's large adaptive radiation, leading to low levels of divergence in their DNA sequences (see Palma-Silva et al 2006;Barbará et al 2007b).…”

Section: Resultsmentioning

confidence: 70%

Transferability of nuclear microsatellite markers to Stigmatodon species: a bromeliad genus endemic to vertical cliffs of inselbergs in Brazil

et al. 2020

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Stigmatodon Leme, G.K.Br. & Barfuss is one of the most representative Bromeliaceae genera that grow on the steepest slopes of the inselbergs of southeastern Brazil. Inselberg environments are characterized as islands due to their restricted, disjunct distribution and geographical isolation. These features make these sites interesting models for evolutionary and population genetic studies. In this study we report, the cross-species transferability of nuclear microsatellite markers, originally developed for other Bromeliaceae, to four Stigmatodon species: Stigmatodon brassicoides, S. costae, S. goniorachis and Stigmatodon sp. nov. Twenty-four microsatellite markers were tested, and 12 of these were successfully amplified in the four studied species. In S. brassicoides and S. costae, 10 markers showed amplification with polymorphism, and in Stigmatodon sp. nov and S. goniorachis, all 12 markers showed polymorphisms. The number of alleles ranged from two to four in S. brassicoides; from two to three in S. costae; from two to six in Stigmatodon sp. nov; and from two to seven in S. goniorachis. H O values ranged from 0.05 (P2P19 locus) in S. brassicoides to 0.689 (VgA04 locus) in S. goniorachis. The set of amplified microsatellite markers described in this study will be a useful tool for addressing various evolutionary, ecological and conservation aspects of Stigmatodon species.

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Patchily distributed but not necessarily isolated populations ofDeuterocohnia meziana: a threatened bromeliad from rock outcrops

Vicente-Silva

Godoy

Faggioni

et al. 2021

Botanical Journal of the Linnean Society

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The movement of organisms or genes among resource patches is fundamental to the persistence of natural populations over time. Species occurring in rock outcrops may have difficulty maintaining genetic connectivity due to the natural disjunct distribution of these formations, which may lead to genetic differentiation. In this study, we tested the hypothesis of genetic isolation among populations of Deuterocohnia meziana on rock outcrops in Brazil. We evaluated genetic diversity and structure, number of migrants, and the contribution of pollen and seed to dispersal. Additionally, we used niche modelling to estimate the potential distribution of D. meziana and combined this with genetic data to identify potential dispersal routes under different climatic scenarios from the Quaternary. Deuterocohnia meziana showed high diversity and moderate genetic structure, low number of migrants and more efficient dispersal by pollen. Only populations separated by > 70 km showed genetic differentiation. Dispersal routes varied mainly in the intensity of the environmental suitability and indicated the present scenario as the most favourable for gene flow in D. meziana. Our findings contribute to the understanding of processes related to the connectivity and isolation of naturally fragmented populations, highlighting the role of biotic and abiotic drivers for genetic differentiation.

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Development of 15 nuclear microsatellite markers in Deuterocohnia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing

Cited by 3 publications

References 16 publications

Cross-amplification of nuclear microsatellite markers in Aechmea distichantha Lem. (Bromeliaceae)

Cross-amplification of nuclear microsatellite markers in Aechmea distichantha Lem. (Bromeliaceae)

Transferability of nuclear microsatellite markers to Stigmatodon species: a bromeliad genus endemic to vertical cliffs of inselbergs in Brazil

Patchily distributed but not necessarily isolated populations ofDeuterocohnia meziana: a threatened bromeliad from rock outcrops

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